Betacellulin/BTC Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Betacellulin/BTC Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human Betacellulin (R&D Systems, Catalog # 261-CE)
  Asp32-Tyr111
  Accession # P35070
• Specificity: Detects human Betacellulin in ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human (rh) HB-EGF, rhEGF, rhTGF-alpha , rhHRG-alpha , and rhAmphiregulin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BTC
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human Betacellulin/BTC Antibody (Catalog # MAB261)
  ELISA Detection: Human Betacellulin/BTC Biotinylated Antibody (Catalog # BAF261)
  Standard: Recombinant Human Betacellulin/BTC (Catalog # 261-CE)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Betacellulin/BTC Antibody [Biotin]

• Betacellulin
• BTC
• probetacellulin

Background

Betacellulin (BTC) is a member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGF-alpha , amphiregulin, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF-7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Betacellulin/BTC Antibody (BAF261)(5)

Publications using BAF261

• LMD Canto, SS Cury, MC Barros-Fil, BEC Kupper, MDFS Begnami, C Scapulatem, RF Carvalho, FA Marchi, DA Olsen, JS Madsen, BM Havelund, S Aguiar, SR Rogatto Locally advanced rectal cancer transcriptomic-based secretome analysis reveals novel biomarkers useful to identify patients according to neoadjuvant chemoradiotherapy response Sci Rep, 2019-06-18;9(1):8702. 2019-06-18 [PMID: 31213644] (ELISA Detection, Human)
• Tom J Parry Effects of neuregulin GGF2 (cimaglermin alfa) dose and treatment frequency on left ventricular function in rats following myocardial infarction Eur. J. Pharmacol, 2016-12-16;796(0):76-89. 2016-12-16 [PMID: 27993643] (Competitive Binding Assay, Human)
• Iaci JF, Ganguly A, Finklestein SP, Parry TJ, Ren J, Saha S, Sietsma DK, Srinivas M, Vecchione AM, Caggiano AO Glial growth factor 2 promotes functional recovery with treatment initiated up to 7 days after permanent focal ischemic stroke. Neuropharmacology, 2010-08-04;59(7):640-9. 2010-08-04 [PMID: 20691195] (ELISA Development, Human)
• Shin S, Li N, Kobayashi N, Yoon JW, Jun HS Remission of diabetes by beta-cell regeneration in diabetic mice treated with a recombinant adenovirus expressing betacellulin. Mol. Ther., 2008-03-18;16(5):854-61. 2008-03-18 [PMID: 18388935] (ELISA Development, Human)
• Sanderson MP, Abbott CA, Tada H, Seno M, Dempsey PJ, Dunbar AJ Hydrogen peroxide and endothelin-1 are novel activators of betacellulin ectodomain shedding. J. Cell. Biochem., 2006-10-01;99(2):609-23. 2006-10-01 [PMID: 16676357] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: BTC
• Uniprot:
  • P35070()