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HAF017). A specific band was detected for Betacellulin/BTC at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
261-CE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Betacellulin/BTC (1 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human Betacellulin/ BTC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-261-NA). The ND50 is typically 0.04-0.08 µg/mL." class="big_lightbox" target="_blank">
261-CE) was serially diluted and captured by Mouse Anti-Human Betacellulin/BTC Monoclonal Antibody (Catalog # MAB261) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Betacellulin/BTC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-261-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
1025-CE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Betacellulin/BTC (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Betacellulin/ BTC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1025). The ND50 is typically 0.1-0.4 µg/mL." class="big_lightbox" target="_blank">
1025-CE) was serially diluted and captured by Rat Anti-Mouse Betacellulin/BTC Monoclonal Antibody (Catalog # MAB10251) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Betacellulin/BTC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1025) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
BT-BTC-GMP), Animal-Free (BT-BTC-AFL) and RUO (Catalog # BT-BTC) grades of Recombinant Human Betacellulin/BTC as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells (orange, green, red, respectively)." class="big_lightbox" target="_blank">
Betacellulin (BTC) is a member of the EGF (epidermal growth factor) family. The transmembrane BTC precursor undergoes proteolytic cleavage, releasing the BTC ligand. It binds to both EGFR/ErbB-1 and ErbB-4 receptor tyrosine kinases. Betacellulin has been found to affect influence various cell types, such as neural stem cells (NSCs), mesenchymal stem cells (MSCs), pancreatic beta cells, retinal pigment epithelial cells and smooth muscle cells.
![Western Blot Betacellulin/BTC Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Betacellulin_AF261NA_Western_Blot_20234.jpg)
![Neutralization Betacellulin/BTC Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Betacellulin_AF1025_Block_Neutralize_8745.jpg)
![Immunohistochemistry Betacellulin/BTC Antibody (34301) [Unconjugated]](http://images.novusbio.com/images/antibody/Betacellulin_MAB2611_Immunohistochemistry_15349.jpg)
![N/A Betacellulin/BTC [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Betacellulin_DY261_ELISA_1856.jpg)
![N/A Betacellulin/BTC [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Betacellulin_DY1025_ELISA_1551.jpg)
![SEC-MALS Betacellulin/BTC [Unconjugated]](http://images.novusbio.com/fullsize/261-ce_recombinant-human-betacellulin-protein-sec-mals-1532024122412..jpg)
![SEC-MALS Betacellulin/BTC [Unconjugated]](http://images.novusbio.com/fullsize/bt-btc_recombinant-human-betacellulin-protein-cf-sec-mals-732024192327..jpg)
![Immunohistochemistry Betacellulin/BTC Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Betacellulin_AF261NA_Immunohistochemistry_17368.jpg)
![Immunohistochemistry Betacellulin/BTC Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Betacellulin_AF261NA_Immunohistochemistry_17238.jpg)
![Neutralization Betacellulin/BTC Antibody [Unconjugated]](http://images.novusbio.com/images/af-261-na_human-betacellulin-affinity-purified-polyclonal-ab-neutralization-639190392498854452.jpg)
![ELISA Betacellulin/BTC Antibody [Unconjugated]](http://images.novusbio.com/images/af-261-na_human-betacellulin-affinity-purified-polyclonal-ab-elisa-639201850082696437.jpg)
![ELISA Betacellulin/BTC Antibody [Unconjugated]](http://images.novusbio.com/images/af1025_mouse-betacellulin-affinity-purified-polyclonal-ab-elisa-639201850082624632.jpg)
![Bioactivity Betacellulin/BTC [Unconjugated]](http://images.novusbio.com/fullsize/bt-btc_recombinant-human-betacellulin-protein-cf-bioactivity-322022153117.jpg)
![SDS-Page Betacellulin/BTC [Unconjugated]](http://images.novusbio.com/fullsize/bt-btc_recombinant-human-betacellulin-protein-cf-sds-page-322022154131.jpg)
![Bioactivity Betacellulin/BTC [Unconjugated]](http://images.novusbio.com/images/bt-btc_recombinant-human-betacellulin-protein-cf-bioactivity-2652022145822.jpg)
