Betacellulin/BTC Antibody [Unconjugated]


Western blot shows lysates of MCF‑7 human breast cancer cell line and OVCAR‑3 human ovarian carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Betacellulin/BTC Antigen more
Betacellulin/BTC was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human Betacellulin/BTC Antigen Affinity-purified Polyclonal Antibody (Catalog #AF-261-NA) at 3 more
Betacellulin/BTC was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human Betacellulin/BTC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-261-NA) at 15 µg/mL more
Recombinant Human Betacellulin/BTC (Catalog #261‑CE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, B/N

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Betacellulin/BTC Antibody [Unconjugated] Summary

E. coli-derived recombinant human Betacellulin
Accession # P35070
Detects Betacellulin/BTC in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 3-15 ug/mL
  • Neutralization 0.04-0.08 ug/mL
  • Western Blot 2 ug/mL
Read Publications using
AF-261-NA in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Betacellulin/BTC Antibody [Unconjugated]

  • Betacellulin
  • BTC
  • probetacellulin


Betacellulin (BTC) is a member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGF-alpha , amphiregulin, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF-7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Betacellulin/BTC Antibody (AF-261-NA)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ELISA Capture, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.

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Showing Publications 1 - 6 of 6.
Publications using AF-261-NA Applications Species
LMD Canto, SS Cury, MC Barros-Fil, BEC Kupper, MDFS Begnami, C Scapulatem, RF Carvalho, FA Marchi, DA Olsen, JS Madsen, BM Havelund, S Aguiar, SR Rogatto Locally advanced rectal cancer transcriptomic-based secretome analysis reveals novel biomarkers useful to identify patients according to neoadjuvant chemoradiotherapy response Sci Rep, 2019-06-18;9(1):8702. 2019-06-18 [PMID: 31213644] (ELISA Capture, Human) ELISA Capture Human
Sun M, Behrens C, Feng L, Ozburn N, Tang X, Yin G, Komaki R, Varella-Garcia M, Hong WK, Aldape KD, Wistuba II HER family receptor abnormalities in lung cancer brain metastases and corresponding primary tumors. Clin. Cancer Res., 2009-07-21;15(15):4829-37. 2009-07-21 [PMID: 19622585] (IHC-P, Human) IHC-P Human
Schneider MR, Mayer-Roenne B, Dahlhoff M, Proell V, Weber K, Wolf E, Erben RG High cortical bone mass phenotype in betacellulin transgenic mice is EGFR dependent. J. Bone Miner. Res., 2009-03-01;24(3):455-67. 2009-03-01 [PMID: 19049329] (IHC, Western Blot, Human) IHC, Western Blot Human
Dahlhoff M, Horst D, Gerhard M, Kolligs FT, Wolf E, Schneider MR Betacellulin stimulates growth of the mouse intestinal epithelium and increases adenoma multiplicity in Apc+/Min mice. FEBS Lett., 2008-07-24;582(19):2911-5. 2008-07-24 [PMID: 18656477] (IHC-P, Human) IHC-P Human
Kansra S, Johnson JL Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK. Mol. Biol. Cell, 2004-07-14;15(9):4299-309. 2004-07-14 [PMID: 15254267] (Neutralization, Human) Neutralization Human
Tanimura K, Nakago S, Murakoshi H, Takekida S, Moriyama T, Matsuo H, Hashimoto K, Maruo T Changes in the expression and cytological localization of betacellulin and its receptors (ErbB-1 and ErbB-4) in the trophoblasts in human placenta over the course of pregnancy. Eur. J. Endocrinol., 2004-07-01;151(1):93-101. 2004-07-01 [PMID: 15248827] (IHC-Fr, Western Blot, Mouse) IHC-Fr, Western Blot Mouse

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Gene Symbol BTC