beta Tubulin Antibody [Biotin]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, ChHa, In, Pm, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

beta Tubulin Antibody [Biotin] Summary

Immunogen
A synthetic peptide made to the N-terminal region of human beta Tubulin (within residues 1-100). [Swiss-Prot P07437]
Localization
Major constituent of microtubules.
Marker
Microtubule Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TUBB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This beta Tubulin antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band at ~50 kDa is seen. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System

Reactivity Notes

Invertebrate reactivity reported in scientific literature (PMID: 28114363)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for beta Tubulin Antibody [Biotin]

  • beta polypeptide
  • MGC117247
  • Tubb5
  • tubulin beta chain
  • tubulin beta polypeptide
  • tubulin beta-1 chain
  • Tubulin beta-5 chain
  • tubulin, beta

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Antibodies against beta Tubulin are useful as loading controls for Western Blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta Tubulin Antibody (NB600-936B). (Showing 1 - 1 of 1 FAQ).

  1. Our customer want to know which tubulin (alpha or beta) is suitable for WB as the loading control in brain tissue. Could you recommend the tubulin alpha or beta as a loading control for WB in brain tissue? In case of adipose tissue, tubulin alpha is not suitable as a loading control. How about in brain tissue? Could you let me know?
    • Both beta and alpha tubulin are expressed in the brain, and either one would make a suitable loading control. Beta tubulin may give a cleaner signal than alpha, but this varies greatly. You may wish to consider NB600-936.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 488 Labeled NB600-936G
DyLight 550 Labeled NB600-936R
DyLight 650 Labeled NB600-936C
HRP Labeled NB600-936H

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Bioinformatics Tool for beta Tubulin Antibody (NB600-936B)

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Blogs on beta Tubulin

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Calculators

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Bioinformatics

Gene Symbol TUBB