beta-III Tubulin Antibody (SDL.3D10)


Western Blot: beta-III Tubulin Antibody (SDL.3D10) [NB120-11314] - Cell line lysates were separated on SDS-PAGE and probed with 1:400 Monoclonal Anti-beta-Tubulin III Clone: DL.3D10. The antibody was developed using more
Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (SDL.3D10) [NB120-11314] - SH-SY-5Y cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:400 more
Western Blot: Tubulin Beta 3 Antibody (SDL.3D10) [NB120-11314] - Produced in mouse

Product Details

Reactivity Hu, Mu, Rt, Bv, Ch(-)Species Glossary
Applications WB, ELISA
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

beta-III Tubulin Antibody (SDL.3D10) Summary

Chemically synthesized peptide corresponding to the carboxyl-terminal sequence of human beta-tubulin isotype III conjugated to BSA.
Ab11314 specifically recognizes an epitope located on human beta-tubulin (isotype III).
Tubulin is the major constituent of microtubules.
Neuronal Marker
may be used for the localization of isotype III of beta-tubulin using various immunochemical assays.
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
15mM Sodium Azide


  • Western Blot 1:400
  • ELISA 1:100-1:2000
Application Notes
Indirect ELISA: Use at an assay dependent dilution. WB: Use at a dilution of 1/400. Predicted molecular weight: 55 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Read Publication using NB120-11314.

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for beta-III Tubulin Antibody (SDL.3D10)

  • beta-4
  • betaIII Tubulin
  • beta-Tubulin III
  • Tubulin Beta 3
  • tubulin beta-3 chain
  • Tubulin beta-4 chain
  • Tubulin beta-4
  • Tubulin beta-III
  • tubulin, beta 3
  • TUJ1 antigen
  • TUJ-1 antigen


Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For beta tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. The monoclonal antibody from hybridoma SDL.3D10 can stimulate microtubule assembly when reconstituted with tubulin, tau or MAP2.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-III Tubulin Antibody (NB120-11314)(1)

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Product General Protocols

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WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on beta-III Tubulin.

Beta III tubulin
The Beta III tubulin protein is abundantly present in both the central and peripheral nervous systems (CNS and PNS), where it is predominantly expressed during fetal and postnatal development. In cerebellar and sympathoadrenal neurogenesis, Beta III d...  Read full blog post.

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Product PDFs


Gene Symbol TUBB3

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