beta-III Tubulin Antibody (SDL.3D10)

Western Blot: beta-III Tubulin Antibody (SDL.3D10) [NB120-11314] - Cell line lysates were separated on SDS-PAGE and probed with 1:400 Monoclonal Anti-beta-Tubulin III Clone: DL.3D10. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. U87 2. MDBK 3. RAT2

Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (SDL.3D10) [NB120-11314] - SH-SY-5Y cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:400 Monoclonal Anti-beta-Tubulin III Clone: SDL.3D10 The antibody was developed using Goat Anti-Mouse IgG, Cy3(r) conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Western Blot: Tubulin Beta 3 Antibody (SDL.3D10) [NB120-11314] - Produced in mouse

Product Discontinued

beta-III Tubulin Antibody (SDL.3D10) Summary

• Immunogen: Chemically synthesized peptide corresponding to the carboxyl-terminal sequence of human beta-tubulin isotype III conjugated to BSA.
• Epitope: Ab11314 specifically recognizes an epitope located on human beta-tubulin (isotype III).
• Localization: Tubulin is the major constituent of microtubules.
• Marker: Neuronal Marker
• Specificity: may be used for the localization of isotype III of beta-tubulin using various immunochemical assays.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBB3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Simple Western
  • Western Blot 1:400
• Application Notes: Indirect ELISA: Use at an assay dependent dilution. WB: Use at a dilution of 1/400. Predicted molecular weight: 55 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  See Simple Western Antibody Database for Simple Western validation: Separated by Size-All, antibody dilution of 1:100; matrix was 12-230 kDa.
• Control:

  beta-III Tubulin Overexpression Lysate

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for beta-III Tubulin Antibody (SDL.3D10)

• Beta3-tubulin
• beta-4
• betaIII Tubulin
• beta-III Tubulin
• beta-Tubulin III
• Class III beta-tubulin
• TUBB3
• TUBB4 CFEOM3A
• Tubulin Beta 3
• tubulin beta-3 chain
• Tubulin beta-4 chain
• Tubulin beta-4
• Tubulin beta-III
• tubulin, beta 3
• TUJ1 antigen
• TUJ-1 antigen

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For beta tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. The monoclonal antibody from hybridoma SDL.3D10 can stimulate microtubule assembly when reconstituted with tubulin, tau or MAP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-III Tubulin Antibody (NB120-11314)(4)

Publications using NB120-11314

• Bonanomi D, Valenza F, Chivatakarn O et al. p190RhoGAP Filters Competing Signals to Resolve Axon Guidance Conflicts Neuron 2019-03-15 [PMID: 30902550] (WB, Mouse)
• Krohn M, Wanek T, Menet MC et al. Humanization of the Blood-Brain Barrier Transporter ABCB1 in Mice Disrupts Genomic Locus- Lessons from Three Unsuccessful Approaches. Eur J Microbiol Immunol (Bp). 2018-09-28 [PMID: 30345087] (WB, Mouse)
• Parras A, Anta H, Santos-Galindo M et al. Autism-like phenotype and risk gene mRNA deadenylation by CPEB4 mis-splicing. Nature 2018-08-15 [PMID: 30111840] (WB, Human)
• Bonanomi D, Chivatakarn O, Bai G, Abdesselem H, Lettieri K, Marquardt T, Pierchala BA, Pfaff SL. Ret Is a Multifunctional Coreceptor that Integrates Diffusible- and Contact-Axon Guidance Signals. Cell;148(3):568-82. 2012-02-03 [PMID: 22304922]

Bioinformatics

• Gene Symbol: TUBB3
• Entrez:
  • 10381(Human)
  • 246118(Rat)
• Uniprot:
  • Q13509(Human)