beta-III Tubulin Antibody (SDL.3D10)

Images

 
Western Blot: beta-III Tubulin Antibody (SDL.3D10) [NB120-11314] - Cell line lysates were separated on SDS-PAGE and probed with 1:400 Monoclonal Anti-beta-Tubulin III Clone: DL.3D10. The antibody was developed using ...read more
Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (SDL.3D10) [NB120-11314] - SH-SY-5Y cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:400 ...read more
Western Blot: Tubulin Beta 3 Antibody (SDL.3D10) [NB120-11314] - Produced in mouse

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch(-)Species Glossary
Applications WB, ELISA, ICC/IF
Clone
SDL.3D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

beta-III Tubulin Antibody (SDL.3D10) Summary

Immunogen
Chemically synthesized peptide corresponding to the carboxyl-terminal sequence of human beta-tubulin isotype III conjugated to BSA.
Epitope
Ab11314 specifically recognizes an epitope located on human beta-tubulin (isotype III).
Localization
Tubulin is the major constituent of microtubules.
Marker
Neuronal Marker
Specificity
may be used for the localization of isotype III of beta-tubulin using various immunochemical assays.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TUBB3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:400
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
Application Notes
Indirect ELISA: Use at an assay dependent dilution. WB: Use at a dilution of 1/400. Predicted molecular weight: 55 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Positive Control
Brain Lysate (NB820-59705)
beta-III Tubulin Lysate (NBL1-17440)
Publications
Read Publication using NB120-11314.

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for beta-III Tubulin Antibody (SDL.3D10)

  • beta-4
  • betaIII Tubulin
  • beta-III Tubulin
  • beta-Tubulin III
  • TUBB4 CFEOM3A
  • Tubulin Beta 3
  • tubulin beta-3 chain
  • Tubulin beta-4 chain
  • Tubulin beta-4
  • Tubulin beta-III
  • tubulin, beta 3
  • TUJ1 antigen
  • TUJ-1 antigen

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For beta tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. The monoclonal antibody from hybridoma SDL.3D10 can stimulate microtubule assembly when reconstituted with tubulin, tau or MAP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB120-11314
Species: Hu, Mu, Rt, Bv, Ch(-)
Applications: WB, ELISA, ICC/IF

Publications for beta-III Tubulin Antibody (NB120-11314)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for beta-III Tubulin Antibody (NB120-11314)

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Blogs on beta-III Tubulin.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

Beta III tubulin
The Beta III tubulin protein is abundantly present in both the central and peripheral nervous systems (CNS and PNS), where it is predominantly expressed during fetal and postnatal development. In cerebellar and sympathoadrenal neurogenesis, Beta III d...  Read full blog post.

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Bioinformatics

Gene Symbol TUBB3
Entrez
Uniprot