beta-III Tubulin Antibody (AA10) - Azide Free

Western Blot: beta-III Tubulin Antibody (AA10) [NBP1-30048] - Rat cortex lysate showing specific immunolabeling of the ~55k beta III tubulin protein.

Immunohistochemistry: beta-III Tubulin Antibody (AA10) [NBP1-30048] - Immunostaining of mouse dentate gyrus showing beta III tubulin in red and nuclei in blue. Photo courtesy of Robert Wine.

• Reactivity: Mu, Rt, Hu, Bv, Ca, Eq, Fe, Gt, Gp, Ha, Pm, Rb, Sh, Vo
• Applications: WB, ICC/IF, IHC
• Clone: AA10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free

beta-III Tubulin Antibody (AA10) - Azide Free Summary

• Description: Novus Biologicals Mouse beta-III Tubulin Antibody (AA10) - Azide Free (NBP1-30048) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-beta-III Tubulin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to amino acid residues specific to beta III tubulin conjugated to KLH. Accession # Q4QRB4
• Marker: Neuronal Marker
• Specificity: Specific for the ~55kDa beta III tubulin protein. This clone is similar to the monoclonal antibody Tuj1.
• Predicted Species: Human (100%), Sheep (100%), Hamster (100%), Goat (100%), Canine (100%), Vole (100%), Equine (100%), Primate (100%), Feline (100%), Rabbit (100%), Guinea Pig (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBB3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:10000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody has been directly tested for reactivity in rat. It is expected that this antibody will have wide species cross reactivity.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for beta-III Tubulin Antibody (AA10) - Azide Free

• Beta3-tubulin
• beta-4
• betaIII Tubulin
• beta-III Tubulin
• beta-Tubulin III
• Class III beta-tubulin
• TUBB3
• TUBB4 CFEOM3A
• Tubulin Beta 3
• tubulin beta-3 chain
• Tubulin beta-4 chain
• Tubulin beta-4
• Tubulin beta-III
• tubulin, beta 3
• TUJ1 antigen
• TUJ-1 antigen

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For beta tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. The monoclonal antibody from hybridoma SDL.3D10 can stimulate microtubule assembly when reconstituted with tubulin, tau or MAP2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-III Tubulin Antibody (NBP1-30048)(1)

Publication using NBP1-30048

• Kawano T, Zhou J, Anwar S et al. T cell infiltration into the brain triggers pulmonary dysfunction in murine Cryptococcus-associated IRIS Nature communications 2023-06-28 [PMID: 37380639] (ICC/IF, Mouse)
  
  Details:
  1:200 ICC/IF dilution

Bioinformatics

• Gene Symbol: TUBB3
• Uniprot:
  • Q4QRB4(Rat)