beta II Tubulin B Antibody (5B2) - BSA Free

Western Blot: beta II Tubulin B Antibody (5B2) [NBP1-74037] - SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin (1:10000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry/Immunofluorescence: beta II Tubulin B Antibody (5B2) [NBP1-74037] - Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:1000) with Texas Red (Red).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 5B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

beta II Tubulin B Antibody (5B2) - BSA Free Summary

• Description: Novus Biologicals Mouse beta II Tubulin B Antibody (5B2) - BSA Free (NBP1-74037) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human beta II Tubulin B (1-445aa) purified from E. coli
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBB2B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Western Blot 1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for beta II Tubulin B Antibody (5B2) - BSA Free

• bA506K6.1
• beta II Tubulin B
• class II beta-tubulin isotype
• DKFZp566F223
• FLJ98847
• MGC8685
• TUBB2B
• tubulin beta-2B chain
• tubulin, beta 2B
• tubulin, beta polypeptide paralog

Background

Tubulin is a major cytoskeleton component that has five distinct forms. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBB2B
• Uniprot:
  • NP_821080(Human)