beta II Tubulin B Antibody (5B2)

Images

 
Western Blot: beta II Tubulin B Antibody (AT5B2) [NBP1-74037] - SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin (1:10000). Proteins were visualized using a goat anti-mouse secondary ...read more
Immunocytochemistry/ Immunofluorescence: beta II Tubulin B Antibody (AT5B2) [NBP1-74037] - Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
5B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

beta II Tubulin B Antibody (5B2) Summary

Immunogen
Recombinant human beta-Tubulin (1-445aa) purified from E. coli
Specificity
Anti-human beta-Tubulin mAb, clone 5B2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human beta-Tubulin protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TUBB2B
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:1000
Application Notes
The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:1000 ~ 5000. Recommended starting dilution is 1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for beta II Tubulin B Antibody (5B2)

  • bA506K6.1
  • class II beta-tubulin isotype
  • DKFZp566F223
  • FLJ98847
  • MGC8685
  • tubulin beta-2B chain
  • tubulin, beta 2B
  • tubulin, beta polypeptide paralog

Background

Tubulin is a major cytoskeleton component that has five distinct forms. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB1195
Species: Multi
Applications: WB, Simple Western, ICC
NB100-690
Species: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Fu, GP, Ha, Mk, Pa, Pm, Rb, Xp, Ye
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, CyTOF-ready, IF
NB600-936
Species: Hu, Mu, Rt, Po, Bv, Ch, ChHa, In, Pm, Xp, Ze
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-00812
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-02474
Species: Hu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
NBP2-15581
Species: Hu, Mu, Rt, Dr
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89483
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB300-213
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-Fr
AF3587
Species: Hu
Applications: WB, IHC, IP, CyTOF-ready, ICC, ICFlow
NBP2-03891
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NB500-317
Species: Hu, Bv(-), Ca(-), Ch(-), Mu(-), Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA
PPS010
Species: Hu, Mu, Rt, Bv, Ch, Xp, Ze
Applications: WB
AF3795
Species: Hu
Applications: WB, IP
NBP1-31733
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-06529
Species: Hu
Applications: WB, IHC-P, IP, PLA
NBP1-83976
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-30954
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for beta II Tubulin B Antibody (NBP1-74037) (0)

There are no publications for beta II Tubulin B Antibody (NBP1-74037).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for beta II Tubulin B Antibody (NBP1-74037) (0)

There are no reviews for beta II Tubulin B Antibody (NBP1-74037). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta II Tubulin B Antibody (NBP1-74037) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional beta II Tubulin B Products

Bioinformatics Tool for beta II Tubulin B Antibody (NBP1-74037)

Discover related pathways, diseases and genes to beta II Tubulin B Antibody (NBP1-74037). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for beta II Tubulin B Antibody (NBP1-74037)

Discover more about diseases related to beta II Tubulin B Antibody (NBP1-74037).
 

Pathways for beta II Tubulin B Antibody (NBP1-74037)

View related products by pathway.

Blogs on beta II Tubulin B

There are no specific blogs for beta II Tubulin B, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our beta II Tubulin B Antibody (5B2) and receive a gift card or discount.

Bioinformatics

Gene Symbol TUBB2B
Uniprot