MYL4 Antibody

Images

 
Western Blot: MYL4 Antibody [NBP2-30954] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: HEL
Immunocytochemistry/ Immunofluorescence: MYL4 Antibody [NBP2-30954] - Staining of human cell line U-2 OS shows localization to plasma membrane.
Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry: MYL4 Antibody [NBP2-30954] - Staining of human heart muscle shows strong cytoplasmic positivity in subsets of myocytes.
Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining in human heart muscle and skeletal muscle tissues using anti-MYL4 antibody. Corresponding MYL4 RNA-seq data are presented for the same tissues.
Simple Western: MYL4 Antibody [NBP2-30954] - Simple Western lane view shows a specific band for MYL4 in 0.2 mg/ml of HEL (left) and h. Heart (right) lysate(s). This experiment was performed under reducing conditions ...read more
Simple Western: MYL4 Antibody [NBP2-30954] - Electropherogram image of the corresponding Simple Western lane view. MYL4 antibody was used at 1:50 dilution on HEL and h. Heart lysate(s) respectively.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MYL4 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PAPEAPKEPAFDPKSVKIDFTADQIE
Specificity
Specificity of human MYL4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYL4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MYL4 Protein (NBP2-30954PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MYL4 Antibody

  • ALC1
  • AMLC
  • GT1
  • MLC1
  • Myosin light chain 1, embryonic muscle/atrial isoform
  • myosin light chain 4
  • Myosin light chain alkali GT-1 isoform
  • myosin, atrial/fetal muscle, light chain
  • myosin, light chain 4, alkali; atrial, embryonic
  • myosin, light polypeptide 4, alkali; atrial, embryonic
  • PRO1957

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MYL4
Uniprot