Tubulin Beta 4 Antibody (OTI3F1)

Western Blot: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] Analysis of extracts (35ug) from 9 different cell lines by using anti-Tubulin Beta 4 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Tubulin Beta 4.

Immunohistochemistry-Paraffin: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Staining of paraffin-embedded Human tonsil using anti-Tubulin Beta 4 mouse monoclonal antibody.

Flow Cytometry: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Analysis of Hela cells, using anti-Tubulin Beta 4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tubulin Beta 4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Tubulin Beta 4.

Immunohistochemistry-Paraffin: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Tubulin Beta 4 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Tubulin Beta 4 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Tubulin Beta 4 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Tubulin Beta 4 Antibody (3F1) [NBP2-00812] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Tubulin Beta 4 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI3F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Tubulin Beta 4 Antibody (OTI3F1) Summary

• Immunogen: Full length human recombinant protein of human TUBB4 (NP_006078) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBB4A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 49.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Tubulin Beta 4 Antibody (OTI3F1)

• TUBB4
• TUBB4A
• TUBB5beta-5
• Tubulin 5 beta
• Tubulin Beta 4
• tubulin beta-4 chain
• tubulin, beta 4
• tubulin, beta, 5

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tubulin Beta 4 Antibody (NBP2-00812)(4)

Publications using NBP2-00812

• Powell H INFLUENZA A VIRUS FITNESS IS LINKED WITH ANTIGENIC CHANGES IN THE HEMAGGLUTININ AND NEURAMINIDASE PROTEINS Thesis 2020-01-01
• Resnick JD, Wilson JL, Anaya E et al. Growth media affects susceptibility of air-lifted human nasal epithelial cell cultures to SARS-CoV2, but not Influenza A, virus infection bioRxiv 2023-08-06 [PMID: 37577692] (Western Blot)
• Powell H, Liu H, Pekosz A Changes in sialic acid binding associated with egg adaptation decrease live attenuated influenza virus replication in human nasal epithelial cell cultures Vaccine 2021-05-10 [PMID: 33985852]
• Tan LX, Toops KA, Lakkaraju A. Protective responses to sublytic complement in the retinal pigment epithelium Proc. Natl. Acad. Sci. U.S.A. 2016-08-02 [PMID: 27432952] (WB)

Bioinformatics

• Gene Symbol: TUBB4A
• Entrez:
  • 10382(Human)