Beta Ig-h3/TGFBI Antibody

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Beta Ig-h3/TGFBI Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human beta IG-H3
  Gly24-His683
  Accession # Q15582
• Specificity: Detects human beta IG-H3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse beta IG-H3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TGFBI
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Beta Ig-h3/TGFBI Antibody

• beta IGH3
• beta IG-H3
• BIGH3EBMD
• CDB1
• CDG2
• CDGG1transforming growth factor, beta-induced, 68kD
• CSD
• CSD1
• CSD2
• CSD3
• Kerato-epithelin
• LCD1
• RGD-CAP
• RGD-containing collagen-associated protein
• TGFBI
• TGFBIP
• transforming growth factor, beta-induced, 68kDa
• transforming growth factor-beta-induced protein ig-h3

Background

Beta IG-H3, also known as TGFBI and RGD-CAP, is a matricellular adaptor protein that is induced in most cell types in response to TGF-beta stimulation (1-4). The human beta IG-H3 cDNA encodes a 683 amino acid (aa) precursor that includes a 23 aa signal sequence, one EMI domain, four FAS1 domains, and one RGD motif (1). Human beta IG-H3 shares 91% and 93% aa sequence identity with mouse and porcine beta IG-H3, respectively. beta IG-H3 is expressed as a 75 kDa protein with no post-translational additions (5). Following secretion, cleavages at multiple positions near the C-terminal end liberate peptides with pro-apoptotic activity (5,6). Peptides that encompass the RGD motif contribute to the pro-apoptotic effects of TGF-beta (6). FAS1 domains contain YH motifs that are characterized by conserved Tyr and His residues (7). The YH motifs in each of the FAS1 domains enable beta IG-H3 binding to matrix Fibronectin, Collagen I, and Collagen VI (3, 8-10) in addition to cell expressed Integrins alpha V beta 3 alpha V beta 5 and alpha 3 beta 1 (7, 8, 11, 12). The expression of beta IG-H3 is modulated at particular developmental stages in some cell types. It is upregulated in keratinocytes and immature dendritic cells but downregulated in osteoblasts (8, 11, 13). It promotes keratinocyte differentiation but blocks osteoblast differentiation (8,11). beta IG-H3 stimulates macrophage endocytosis and vascular endothelial cell proliferation and migration (12, 13). High glucose levels induce beta IG-H3 in renal proximal tubule cells which is predictive of diabetic nephropathy (3). Several point mutations (clustered in the fourth FAS1 domain) of beta IG-H3 are linked to different corneal dystrophies (14). beta IG-H3 is downregulated in many cancers (4, 15) and functions as a suppressor of tumorigenicity when overexpressed (2, 4, 15).

1. Skonier, J. et al. (1992) DNA Cell Biol. 11:511.
2. Skonier, J. et al. (1994) DNA Cell Biol. 13:571.
3. Lee, S-H. et al. (2003) Kidney Int. 64:1012.
4. Zhao, Y.L. et al. (2002) Oncogene 21:7471.
5. Andersen, R.B. et al. (2004) Biochemistry 43:16374.
6. Kim, J-E. et al. (2003) Oncogene 22:2045.
7. Kim, J-E. et al. (2002) J. Biol. Chem. 277:46159.
8. Thapa, N. et al. (2005) Bone 36:232.
9. Hanssen, E. et al. (2003) J. Biol. Chem. 278:24334.
10. Billings, P.C. et al. (2002) J. Biol. Chem. 277:28003.
11. Oh, J-E. et al. (2005) J. Biol. Chem. 280:21629.
12. Nam, J-O. et al. (2003) J. Biol. Chem. 278:25902.
13. Cao, W, et al. (2006) Blood 107:2777. 
14. Stewart, H.S. et al. (1999) Hum. Mutat. 14:126.
15. Zhao, Y. et al. (2006) Mol. Carcinog. 45:84.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Beta Ig-h3/TGFBI Antibody (AF2935)(6)

Publications using AF2935

• Dean E. Hammond, J. Dinesh Kumar, Lorna Raymond, Deborah M. Simpson, Robert J. Beynon, Graham J. Dockray et al. Stable Isotope Dynamic Labeling of Secretomes (SIDLS) Identifies Authentic Secretory Proteins Released by Cancer and Stromal Cells* Molecular & Cellular Proteomics 9/1/2018 [PMID: 29915148]
• C Hoffmann, S Höckele, L Kappler, M Hrab? de A, HU Häring, C Weigert The effect of differentiation and TGF? on mitochondrial respiration and mitochondrial enzyme abundance in cultured primary human skeletal muscle cells Sci Rep, 2018-01-15;8(1):737. 1/15/2018 [PMID: 29335583]
• Yong-Sun Maeng, Ga-Hyun Lee, Seung-Il Choi, Kyu Seo Kim, Eung Kweon Kim Histone methylation levels correlate with TGFBIp and extracellular matrix gene expression in normal and granular corneal dystrophy type 2 corneal fibroblasts BMC Medical Genomics 12/1/2015 [PMID: 26553048]
• Choi S, Kim B, Dadakhujaev S, Oh J, Kim T, Kim J, Kim E Impaired autophagy and delayed autophagic clearance of transforming growth factor beta-induced protein (TGFBI) in granular corneal dystrophy type 2. Autophagy, 2012-09-20;8(12):1782-97. 2012-09-20 [PMID: 22995918] (Western Blot, Human)
• Kyung Eun Han, Seung-il Choi, Woo Suk Chung, Se Hwan Jung, Nicholas Katsanis, Tae-im Kim et al. Extremely varied phenotypes in granular corneal dystrophy type 2 heterozygotes Mol. Vis 6/27/2012 [PMID: 22815629]
• Choi SI, Kim TI, Kim KS, Kim BY, Ahn SY, Cho HJ, Lee HK, Cho HS, Kim EK Decreased catalase expression and increased susceptibility to oxidative stress in primary cultured corneal fibroblasts from patients with granular corneal dystrophy type II. Am. J. Pathol., 2009-06-04;175(1):248-61. 2009-06-04 [PMID: 19497990] (Western Blot, Human)

Bioinformatics

• Gene Symbol: TGFBI
• Uniprot:
  • Q15582()