beta-Catenin Antibody

Images

 
Western Blot: beta-Catenin Antibody [NBP1-32239] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term diluted at 1:3000.
Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - Paraformaldehyde-fixed A431, using beta- Catenin antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with an alpha ...read more
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - WNT10A/beta-catenin signaling is required for region-specific differentiation. Epithelial deletion of beta-catenin (g-h",k,l) induced from P25, P110 or ...read more
Flow Cytometry: beta-Catenin Antibody [NBP1-32239] - HeLa cell.Black: Unlabelled sample was used as a control. Red: beta Catenin antibody [N1N2-2], N-term dilution: 1:50.Acquisition of 20,000 events were collected using ...read more
Chromatin Immunoprecipitation (ChIP): beta-Catenin Antibody [NBP1-32239] - Cross-linked ChIP was performed with HCT116 chromatin extract and 5 ug of either control rabbit IgG or anti-beta Catenin antibody. The ...read more
Western Blot: beta-Catenin Antibody [NBP1-32239] - Western blot for beta-catenin in HEK 293 cells treated with LiCl (10mM) for 1 hr. Image from verified customer review.
Western Blot: beta-Catenin Antibody [NBP1-32239] - Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000
Western Blot: beta-Catenin Antibody [NBP1-32239] - A. 30 ug PC-12 whole cell lysate/extract 7.5 % SDS-PAGEbeta Catenin antibody [N1N2-2], N-term dilution: 1:1000
Genetic Strategies: Western Blot: beta-Catenin Antibody [NBP1-32239] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ...read more
Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term ...read more
Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], ...read more
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse colon. Green: beta Catenin antibody [diluted at 1:500. Red: alpha Tubulin antibody diluted at 1:500. Blue: Hoechst 33342 ...read more
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse urinary bladder diluted at 1:500.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse skin dilution: 1:500.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse colon dilution: 1:500.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded human esophagus. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded human cervix. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded rat colon. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded rat duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse intestine. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.
Immunoblotting analysis of IR-induced AKT (Ser473) activation. (A) The levels of AKT activation were measured by AKT Ser473 phosphorylation in 293T, 293T/eIF3f and 293T/eIF3f-hMSH4 cells treated with 10 Gy IR in ...read more
AMPK‐activating AICAR treatment activates FANCD2, a pivotal molecule of Fanconi anemia DNA damage signaling pathway. (A) AICAR treatment induces FANCD2 monoubiquitination in transformed normal fibroblasts (GM00637I). ...read more
Roles of P38 mitogen-activated protein kinase (MAPK) in the DFO-mediated up-regulate of HIF-1 alpha protein in APPsw cells. (A) APPsw were exposed to 100 μM Fe, 100 μM DFO, or both combined for 24 h. Whole cell ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Fe, Rb, Ze, Po, Bv, Ch, RM, Sh, XpSpecies Glossary
Applications WB, Flow, Func, ICC/IF, IHC, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

beta-Catenin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary.
Localization
Cytoplasm , Nucleus , cytoskeleton
Marker
Epithelial Cell Marker, Adherens Junction Marker
Predicted Species
Porcine (99%), Bovine (99%), Rhesus Macaque (100%), Chicken (97%), Sheep (99%), Xenopus (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CTNNB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:50-1:100
  • Proximity Ligation Assay
  • Western Blot 1:500-1:20000
Application Notes
Use in proximity ligation assay reported in scientific literature (PMID 28589954). ChIP assay- Assay dependent.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-32239 in the following applications:

Publications
Read Publications using
NBP1-32239 in the following applications:

Reactivity Notes

Xenopus laevis (97%), Cat (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for beta-Catenin Antibody

  • bCatenin
  • beta 1 (88kD)
  • beta-Catenin
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • catenin beta-1
  • CTNNB
  • CTNNB1
  • DKFZp686D02253
  • FLJ25606
  • FLJ37923

Background

The distinct peripheral cytosolic proteins, alpha, beta and gamma catenin (102, 94 and 86 kDa) are found in many tissues bind to the conserved cytoplasmic tail domain of the cell adhesion cadherins. Catenins link E cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt1 proto oncogene. The central core region of beta catenin is involved in mediation of cadherin catenin complex interaction with EGFR. Beta-Catenin-mediated signalling is involved at several stages of vertebrate neural development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-Catenin Antibody (NBP1-32239)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Chemotaxis, ICC/IF, IF/IHC, IP, PLA, WB.


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Chemotaxis
(1)
ICC/IF
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IF/IHC
(1)
IP
(2)
PLA
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(4)
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Human
(2)
Mouse
(4)
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Showing Publications 1 - 8 of 8.
Publications using NBP1-32239 Applications Species
Buechel D, Sugiyama N, Rubinstein N Et al. Parsing beta-catenin's cell adhesion and Wnt signaling functions in malignant mammary tumor progression Proceedings of the National Academy of Sciences of the United States of America 2021-08-24 [PMID: 34408016] (IP, Mouse) IP Mouse
Yang X, Chung JY, Rai U, Esumi N. Cadherins in the retinal pigment epithelium (RPE) revisited: P-cadherin is the highly dominant cadherin expressed in human and mouse RPE in vivo PLoS ONE 2018-01-16 [PMID: 29338041] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Xu M, Horrell J, Snitow M et al. WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation. Nat Commun. 2017-06-07 [PMID: 28589954] (PLA, Mouse) PLA Mouse
Jin X, Li T, Zhang L et al. Environmental Enrichment Improves Spatial Learning and Memory in Vascular Dementia Rats with Activation of Wnt/b-Catenin Signal Pathway. Med. Sci. Monit. 2017-01-13 [PMID: 28082734] (WB, Rat) WB Rat
Buchel DM Wnt/beta-catenin signaling in malignant mammary tumor progression and metastasis formation & Mechanisms of evasive resistance to sorafenib in hepatocellular carcinoma Thesis (Chemotaxis, IP, Human) Chemotaxis, IP Human
Borrelli C, Valenta T, Handler K et al. Differential regulation of beta-catenin-mediated transcription via N- and C-terminal co-factors governs identity of murine intestinal epithelial stem cells Nature communications 2021-03-01 [PMID: 33649334] (WB) WB
Cao J, Liu D, Zhao S et al. Estrogen attenuates TGF-beta 1-induced EMT in intrauterine adhesion by activating Wnt/beta-catenin signaling pathway Braz. J. Med. Biol. Res. 2020-07-06 [PMID: 32638833] (WB, Human) WB Human
Saxena M, Kalathur RKR, Rubinstein N et al. A Pygopus 2-histone interaction is critical for cancer cell de-differentiation and progression in malignant breast cancer Cancer Res. 2020-06-25 [PMID: 32586983] (IF/IHC, Mouse) IF/IHC Mouse

Review for beta-Catenin Antibody (NBP1-32239) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-32239:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot beta-Catenin NBP1-32239
Enlarge
5
reviewed by:
Verified Customer
WB Human 06/25/2014
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Summary

ApplicationWestern Blot
Sample TestedLysate from HEK293 cells
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Catenin Antibody (NBP1-32239) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Research Areas for beta-Catenin Antibody (NBP1-32239)

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Blogs on beta-Catenin.


  Read full blog post.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

The role of Wnts in neuroinflammation
By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s...  Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Notch1 - A multifunctional transmembrane receptor
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Verified Customer
06/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol CTNNB1