beta-Actin Antibody (1104805) [Alexa Fluor® 647]

• Reactivity: Mu
• Clone: 1104805
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 647

beta-Actin Antibody (1104805) [Alexa Fluor® 647] Summary

• Immunogen: Synthetic Peptide
  Accession # P60710
• Specificity: Detects a synthetic peptide specific for mouse beta-actin around amino acid 50 in Direct ELISA.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-Actin Antibody (1104805) [Alexa Fluor® 647]

• ACTB
• actin, beta
• actin, cytoplasmic 1
• B-Actin
• Beta Actin
• beta cytoskeletal actin
• betaActin
• beta-Actin
• BRWS1
• I(2)-Actin
• PS1TP5-binding protein 1
• PS1TP5BP1

Background

Beta actin (ACTB) is a canonical member of the actin family of proteins, with a molecular weight of approximately 42 kDa. Actins are highly conserved proteins that play pivotal roles in various cellular processes, including the maintenance of the cytoskeleton and cell motility, division, and signaling. ACTB is ubiquitously expressed in all eukaryotic cells and is crucial for structural integrity and motility. It is involved in essential biological processes such as cellular trafficking and the maintenance of cell shape and polarity. Dysregulation of ACTB expression or function can lead to notable pathologies, including cancer progression, where it is implicated in tumor cell migration, invasion, and metastasis. Mutations in the ACTB gene are also associated with developmental disorders and conditions like Baraitser-Winter syndrome. Additionally, ACTB plays a critical role in intracellular transport processes and the functioning of the actin cytoskeleton, highlighting its potential as a biomarker for various diseases and a target for therapeutic interventions.

1. Pollard, T. D., & Cooper, J. A. (2009). Actin, a central player in cell shape and movement. Science, 326(5957), 1208-1212. doi: 10.1126/science.1175862.
2. Perrin, B. J., & Ervasti, J. M. (2010). The actin gene family: Function follows isoform. Cytoskeleton, 67(10), 630-634. doi: 10.1002/cm.20475.
3. Jones, S. L., Korobova, F., Svitkina, T. (2019). Origin of the actin cytoskeleton. Nature Reviews Molecular Cell Biology, 20(11), 675-689. doi: 10.1038/s41580-019-0164-5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for beta-Actin Antibody (FAB11702R). (Showing 1 - 5 of 5 FAQ).

1. Why is beta actin used as a loading control in blotting ?
   • Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
2. Are the beta-Actin antibodies validated in Simple Western?
   • Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
3. Do Beta-Actin antibodies come in lyophilized format?
   • ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
4. What is the theoretical molecular weight for Beta-Actin antibodies?
   • The TMW for beta Actin antibodies is approximately 42 kDa.
5. I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
   • For homogenized tissue, beta-actin and GAPDH are both fine.

Bioinformatics

• Uniprot:
  • P60710(Mouse)