BDNF Antibody (35928) [Unconjugated]

BDNF was detected in immersion fixed paraffin-embedded sections of human spinal cord using Human BDNF Monoclonal Antibody (Catalog # MAB248) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 35928
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

BDNF Antibody (35928) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human BDNF
  Arg128-Arg247
  Accession # P23560
• Specificity: Detects human BDNF in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 5% cross-reactivity with recombinant human (rh) beta -NGF and rr beta -NGF.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BDNF

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BDNF Antibody (35928) [Unconjugated]

• Abrineurin
• ANON2
• BDNF
• brain-derived neurotrophic factor
• BULN2
• MGC34632
• Neurotrophin

Background

Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.

BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye, and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung, and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
2. Snider, W.D. (1994) Cell 77:627.
3. Barbacid, M. (1994) J. Neurobiol. 25:1386.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BDNF Antibody (MAB248)(8)

Publications using MAB248

• Todoroki K, Ogasawara S, Akiba J, Nakayama M, Naito Y, Seki N, Kusukawa J, Yano H CD44v3+/CD24- cells possess cancer stem cell-like properties in human oral squamous cell carcinoma. Int J Oncol, 2015;48(1):99-109. 2015 [PMID: 26647656] (IHC-P, Human)
• Vanhecke E, Adriaenssens E, Verbeke S, Meignan S, Germain E, Berteaux N, Nurcombe V, Le Bourhis X, Hondermarck H Brain-derived neurotrophic factor and neurotrophin-4/5 are expressed in breast cancer and can be targeted to inhibit tumor cell survival. Clin. Cancer Res., 2011;17(7):1741-52. 2011 [PMID: 21350004] (Neutralization, Human)
• Diekmann H, Anichtchik O, Fleming A, Futter M, Goldsmith P, Roach A, Rubinsztein DC Decreased BDNF levels are a major contributor to the embryonic phenotype of huntingtin knockdown zebrafish. J. Neurosci., 2009;29(5):1343-9. 2009 [PMID: 19193881] (Western Blot, Zebrafish)
• Sung SY, Hsieh CL, Law A, Zhau HE, Pathak S, Multani AS, Lim S, Coleman IM, Wu LC, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PA, Marshall FF, Chung LW Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis. Cancer Res., 2008;68(23):9996-10003. 2008 [PMID: 19047182] (IHC-P, Human)
• Tang S, Machaalani R, Waters KA Brain-derived neurotrophic factor (BDNF) and TrkB in the piglet brainstem after post-natal nicotine and intermittent hypercapnic hypoxia. Brain Res., 2008;1232(0):195-205. 2008 [PMID: 18674523] (IHC-P, Porcine)
• Berzi A, Ayata CK, Cavalcante P, Falcone C, Candiago E, Motta T, Bernasconi P, Hohlfeld R, Mantegazza R, Meinl E, Farina C BDNF and its receptors in human myasthenic thymus: implications for cell fate in thymic pathology. J. Neuroimmunol., 2008;197(2):128-39. 2008 [PMID: 18555538] (ICC, Human)
• Samah B, Porcheray F, Gras G Neurotrophins modulate monocyte chemotaxis without affecting macrophage function. Clin. Exp. Immunol., 2008;151(3):476-86. 2008 [PMID: 18190610] (ICC, Human)
• Peiris TS, Machaalani R, Waters KA Brain-derived neurotrophic factor mRNA and protein in the piglet brainstem and effects of Intermittent Hypercapnic Hypoxia. Brain Res., 2004;1029(1):11-23. 2004 [PMID: 15533311] (Western Blot, Porcine)

Bioinformatics

• Gene Symbol: BDNF
• Uniprot:
  • P23560()