BCR Antibody (1E11G12) - BSA Free

Western Blot: BCR Antibody (1E11G12) [NBP2-61707] - Analysis using BCR mAb against HEK293 (1) and BCR (AA: 139-280)-hIgGFc transfected HEK293 (2) cell lysate.

Flow Cytometry: BCR Antibody (1E11G12) [NBP2-61707] - Analysis of K562 cells using BCR mouse mAb (green) and negative control (red).

Western Blot: BCR Antibody (1E11G12) [NBP2-61707] - Analysis using BCR mAb against human BCR (AA: 139-280) recombinant protein. (Expected MW is 41.6 kDa)

Western Blot: BCR Antibody (1E11G12) [NBP2-61707] - Analysis using BCR mouse mAb against Jurkat (1), Hela (2), and Ramos (3) cell lysate.

ELISA: BCR Antibody (1E11G12) [NBP2-61707] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clone: 1E11G12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BCR Antibody (1E11G12) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human BCR (AA: 139-280) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCR
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Western Blot 1:500-1:2000
• Theoretical MW: 142.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for BCR Antibody (1E11G12) - BSA Free

• ALL
• ALLD22S11
• BCR
• BCR1
• breakpoint cluster region protein
• breakpoint cluster region
• CMLEC 2.7.11.1
• D22S662
• PHL
• PHLFLJ16453
• Renal carcinoma antigen NY-REN-26

Background

A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCR