BCL2L10 Antibody

Images

 
Western Blot: BCL2L10 Antibody [NBP1-77224] - Analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: BCL2L10 Antibody [NBP1-77224] - Jurkat cells with BCL2L10 antibody at 10 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, ICC, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

BCL2L10 Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human Bcl-B. The immunogen is located within amino acids 90 - 140 of Bcl-B.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL2L10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunocytochemistry
  • Immunofluorescence
Application Notes
This antibody can be used for the detection of Bcl-B by Western blot at 1 - 2 mg/ml.
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
BCL2L10 Peptide (NBP1-77224PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BCL2L10 Antibody

  • Apoptosis regulator Bcl-B
  • apoptotic protein NrH
  • bcl2-L-10
  • BCL2-like 10 (apoptosis facilitator)
  • bcl-2-like protein 10
  • BCLB
  • BCL-B
  • Boo
  • Diva
  • MGC129810
  • MGC129811

Background

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-B is a recently discovered anti-apoptotic member of the Bcl-2. Unlike the mouse homolog (also known as Diva/Boo) which is predominantly expressed in ovary and testis, the human Bcl-B protein is widely expressed. Also, the human Bcl-B functions by binding to and suppressing the apoptotic activity of Bax, whereas the mouse homolog binds Bak and also interacts with the apoptosis protein Apaf-1. Despite its predicted molecular weight, Bcl-B is often at higher molecular weights, presumably due to post-translational modifications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BCL2L10
Uniprot