BCL2L10 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: BCL2L10 Antibody [NBP1-31681] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: BCL2L10 Antibody [NBP1-31681] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.

Western Blot: BCL2L10 Antibody [NBP1-31681] -Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL2L10 antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BCL2L10 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BCL2L10 Antibody - BSA Free (NBP1-31681) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-BCL2L10 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human BCL2L10. The exact sequence is proprietary.
• Localization: Mitochondrion, Nucleus membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCL2L10
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for BCL2L10 Antibody - BSA Free

• Apoptosis regulator Bcl-B
• apoptotic protein NrH
• BCL2L10
• Bcl2-L-10
• BCL2-like 10 (apoptosis facilitator)
• bcl-2-like protein 10
• BCLB
• BCL-B
• BOO
• DIVA
• MGC129810
• MGC129811

Background

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains conserved BH4, BH1 and BH2 domains. This protein can interact with other members of BCL-2 protein family including BCL2, BCL2L1/BCL-X(L), and BAX. Overexpression of this gene has been shown to suppress cell apoptosis possibly through the prevention of cytochrome C release from the mitochondria, and thus activating caspase-3 activation. The mouse counterpart of this protein is found to interact with Apaf1 and forms a protein complex with Caspase 9, which suggests the involvement of this protein in APAF1 and CASPASE 9 related apoptotic pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCL2L10 Antibody (NBP1-31681)(1)

Publication using NBP1-31681

• Ji X, Tao R, Sun LY et al. Down-regulation of long non-coding RNA DUXAP8 suppresses proliferation, metastasis and EMT by modulating miR-498 through TRIM44-mediated AKT/mTOR pathway in non-small-cell lung cancer Eur Rev Med Pharmacol Sci 2020-03-01 [PMID: 32271433] (Human)

Bioinformatics

• Gene Symbol: BCL2L10
• Entrez:
  • 10017(Human)
• Uniprot:
  • Q9HD36(Human)