Rat Bcl-xL ELISA Kit (Colorimetric)

Rat Bcl-xL ELISA Kit (Colorimetric) [NBP2-77043] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, tissue homogenates and cell lysates
• Standard Curve Range: 46.88 pg/mL - 3000 pg/mL
• Sensitivity: 11.72 pg/mL

Rat Bcl-xL ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat Bcl-2. No significant cross-reactivity or interference between Rat Bcl-2 and analogues was observed.
• Standard Curve Range: 46.88 pg/mL - 3000 pg/mL
• Sensitivity: 11.72 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Spike Recovery: 88 - 102%
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: BCL2L1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Bcl-xL ELISA Kit (Colorimetric)

• Apoptosis regulator Bcl-X
• bcl2-L-1
• BCL2-like 1
• bcl-2-like protein 1
• BCLXBCL2LBcl-X
• BclxL
• Bcl-xL
• BCL-XL/S
• BCLXS
• bcl-xS
• DKFZp781P2092

Background

Bcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis (1). Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2 (2). It has been found that eterodimerization with Bax does not seem to be required for anti-apoptotic activity (3). Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival (4). The Bcl-X(S) isoform promotes apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: BCL2L1