Bcl-xL Antibody (C4) - BSA Free

Immunohistochemistry-Paraffin: Bcl-xL Antibody (C4) [NBP3-42083] - DAB staining on IHC-P; Samples: Rat Testis Tissue; Primary Ab: 30ug/ml NBP3-42083; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Western Blot: Bcl-xL Antibody (C4) [NBP3-42083] - Sample: Recombinant Bcl-xL, Rat.

Immunohistochemistry-Paraffin: Bcl-xL Antibody (C4) [NBP3-42083] - DAB staining on IHC-P; Samples: Rat Uterus Tissue; Primary Ab: 30ug/ml NBP3-42083; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

• Reactivity: Hu, Rt
• Applications: WB, IHC
• Clone: C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Bcl-xL Antibody (C4) - BSA Free Summary

• Description: Novus Biologicals Mouse Bcl-xL Antibody (C4) - BSA Free (NBP3-42083) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Immunized with recombinant Rat Bcl-xL (Uniprot#: P53563; Ser2-Arg149 (Uniprot#: P53563)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCL2L1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-20ug/mL
  • Immunohistochemistry-Paraffin 5-20ug/mL
  • Western Blot 0.5-2ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH7.4, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Bcl-xL Antibody (C4) - BSA Free

• Apoptosis regulator Bcl-X
• bcl2-L-1
• BCL2-like 1
• bcl-2-like protein 1
• BCLXBCL2LBcl-X
• BclxL
• Bcl-xL
• BCL-XL/S
• BCLXS
• bcl-xS
• DKFZp781P2092

Background

Bcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis (1). Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2 (2). It has been found that eterodimerization with Bax does not seem to be required for anti-apoptotic activity (3). Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival (4). The Bcl-X(S) isoform promotes apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL2L1