Bcl-2 Antibody (8C8) Summary
A synthetic peptide corresponding to amino acids 41-60 of human Bcl-2 was used as immunogen to generate the antibody; GenBank no. NP_000648.2. This sequence is 100% conserved in the human alpha (239 aa) and beta (205 aa) isoforms.
Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.
Apoptosis & Follicular Lymphoma Marker
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Protein A or G purified
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Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS with 0.05% BSA.
0.05% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Protein A or G purified
Use in Flow Cytometry and Immunocytochemistry/Immunofluorescence reported in literature. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Alternate Names for Bcl-2 Antibody (8C8)
- apoptosis regulator Bcl-2
- B-cell CLL/lymphoma 2
Bcl-2 (Bcl2) is the founding member of the Bcl-2 family. Family members have pivotal roles in regulating apoptosis or death signaling pathways through their control of mitochondrial permeability and cytochrome release (reviewed in Anvekar, 2011; Martinou and Youle, 2011). Bcl-2 derives its name from B cell lymphoma 2 where was first found to be highly expressed in follicular lymphomas with 14;18 reciprocal translocations. There are two isoforms, alpha and beta, generated by alternative splicing and differing in their carboxy termini. Human Bcl-2 alpha is a 239 amino acid (aa) protein and human Bcl-2 beta is a 205 aa protein. Bcl-2 is over expressed in neoplastic germinal centers of a majority of follicular lymphomas, whereas the normal or hyperplastic germinal centers are primarily negative for Bcl-2 expression. Upregulation has also been described in a number of other types of tumors. Bcl-2 expression is often considered to be a marker of cell death status, and over or high expression has often been tied to anti-apoptotic states, or resistance to death. However, the actual status of vulnerability to death can depend on the balance of other Bcl-2 family members present, their interaction with one another, as well as other factors. This antibody has been extensively used to monitor the status of Bcl-2 expression in a myriad of normal and cancerous tissue types, developmental stages, as well as in cultured cells. Researchers are encouraged to review the antibody specific published literature relevant to their studies through a key word search on scholar.google.com.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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