BAMBI/NMA Antibody [Unconjugated]

BAMBI/NMA was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human BAMBI/NMA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF921) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

BAMBI/NMA Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BAMBI/NMA
  Glu27-Ala152
  Accession # Q13145
• Specificity: Detects human BAMBI/NMA in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BAMBI
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BAMBI/NMA Antibody [Unconjugated]

• BAMBI
• BMP and activin membrane-bound inhibitor homolog (Xenopus laevis)
• BMP and activin membrane-bound inhibitor homolog
• NMA
• NMANon-metastatic gene A protein
• Putative transmembrane protein NMA

Background

BAMBI/NMA is a type I transmembrane protein that shares sequence homology with the TGF-beta type I receptor family protein but has a short cytoplasmic region that lacks the kinase domain. It functions as a membrane-bound decoy receptor by competing with type I receptors to form a heterodimer with type II receptors. Mouse BAMBI/NMA share approximately 93% amino acid sequence homology with human BAMBI/NMA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAMBI/NMA Antibody (AF921)(4)

Publications using AF921

• SW Sun, L Chen, M Zhou, JH Wu, ZJ Meng, HL Han, SY Miao, CC Zhu, XZ Xiong BAMBI regulates macrophages inducing the differentiation of Treg through the TGF-? pathway in chronic obstructive pulmonary disease Respir. Res., 2019-02-06;20(1):26. 2019-02-06 [PMID: 30728014] (Western Blot, Human)
• Seok Lee C-terminal-truncated HBV X promotes hepato-oncogenesis through inhibition of tumor-suppressive ?-catenin/BAMBI signaling Exp. Mol. Med, 2016-12-02;48(12):e275. 2016-12-02 [PMID: 27909336] (Western Blot, Human)
• TGF-?/BAMBI pathway dysfunction contributes to peripheral Th17/Treg imbalance in chronic obstructive pulmonary disease Sci Rep, 2016-08-23;6(0):31911. 2016-08-23 [PMID: 27549738] (ICC, Human)
• Luo X, Hutley LJ, Webster JA, Kim YH, Liu DF, Newell FS, Widberg CH, Bachmann A, Turner N, Schmitz-Peiffer C, Prins JB, Yang GS, Whitehead JP Identification of BMP and activin membrane-bound inhibitor (BAMBI) as a potent negative regulator of adipogenesis and modulator of autocrine/paracrine adipogenic factors. Diabetes, 2012-01-01;61(1):124-36. 2012-01-01 [PMID: 22187378] (Western Blot, Human)

Review for BAMBI/NMA Antibody (AF921) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 08/16/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: fibroblasts
• Species: Human
• Lot: FSV0315021

Comments

• Comments: Used at 10ug/mL in : 1% BSA/ 0.3% Triton X-100 in PBS overnight at 4C. Donkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor 555 (used at 1.5ul/mL) 2H, RT in same dilution buffer.

Bioinformatics

• Gene Symbol: BAMBI
• Uniprot:
  • Q13145()