BAMBI/NMA Antibody (3C1-1D1) - Azide and BSA Free

Western Blot: BAMBI/NMA Antibody (3C1-1D1) [H00025805-M01] - Analysis of BAMBI expression in transfected 293T cell line by BAMBI monoclonal antibody (M01), clone 3C1-1D1.Lane 1: BAMBI transfected lysate(29.1 KDa).Lane 2: Non-transfected lysate.

Western Blot: BAMBI/NMA Antibody (3C1-1D1) [H00025805-M01] - BAMBI monoclonal antibody (M01), clone 3C1-1D1 Western Blot analysis of BAMBI expression in K-562 ( Cat # L009V1 ).

ELISA: BAMBI/NMA Antibody (3C1-1D1) [H00025805-M01] - Detection limit for recombinant GST tagged BAMBI is 3 ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3C1-1D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

BAMBI/NMA Antibody (3C1-1D1) - Azide and BSA Free Summary

• Immunogen: BAMBI (AAH19252, 1 a.a. ~ 260 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDRHSSYIFIWLQLELCAMAVLLTKGEIRCYCDAAHCVATGYMCKSELSACFSRLLDPQNSNSPLTHGCLDSLASTTDICQAKQARNHSGTTIPTLECCHEDMCNYRGLHDVLSPPRGEASGQGNRYQHDGSRNLITKVQELTSSKELWFRAAVIAVPIAGGLTLVLLIMLALRMLRSENKRLQDQRQQMLSRLHYSFHGHHSKKGQVAKLDLECMVPVSGHENCCLTCDKMRQADLSNDKILSLVHWGMYSGHGKLEFV
• Localization: Cell Membrane
• Specificity: BAMBI - BMP and activin membrane-bound inhibitor homolog (Xenopus laevis)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BAMBI
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for BAMBI/NMA Antibody (3C1-1D1) - Azide and BSA Free

• BAMBI
• BMP and activin membrane-bound inhibitor homolog (Xenopus laevis)
• BMP and activin membrane-bound inhibitor homolog
• NMA
• NMANon-metastatic gene A protein
• Putative transmembrane protein NMA

Background

This gene encodes a transmembrane glycoprotein related to the type I receptors of the transforming growth factor-beta (TGF-beta) family, whose members play important roles in signal transduction in many developmental and pathological processes. The encoded protein however is a pseudoreceptor, lacking an intracellular serine/threonine kinase domain required for signaling. Similar proteins in frog, mouse and zebrafish function as negative regulators of TGF-beta, which has led to the suggestion that the encoded protein may function to limit the signaling range of the TGF-beta family during early embryogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAMBI/NMA Antibody (H00025805-M01)(6)

Publications using H00025805-M01

• Hernandez H, Millar JC, Curry SM et al. BMP and Activin Membrane Bound Inhibitor Regulates the Extracellular Matrix in the Trabecular Meshwork. Invest Ophthalmol Vis Sci 2018-04-01 [PMID: 29801150]
• Tovar-Vidales T, Fitzgerald AM, Clark AF. Human trabecular meshwork cells express BMP antagonist mRNAs and proteins. Exp Eye Res 2016-05-07 [PMID: 27167364]
• Wanninger J, Neumeier M, Bauer S et al. Adiponectin induces the transforming growth factor decoy receptor BAMBI in human hepatocytes. FEBS Lett. 2011-04-07 [PMID: 21496456]
• Xavier S, Gilbert V, Rastaldi MP et al. BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation. PLoS One 5(9). 2010-09-24 [PMID: 20886049]
• Pils D, Wittinger M, Petz M et al. BAMBI is overexpressed in ovarian cancer and co-translocates with Smads into the nucleus upon TGF-ss treatment. Gynecol Oncol. 2010-02-25 [PMID: 20189233]
• Tramullas M, Lantero A, Diaz A et al. BAMBI (bone morphogenetic protein and activin membrane-bound inhibitor) reveals the involvement of the transforming growth factor-beta family in pain modulation. J Neurosci 30(4):1502-11. 2010-01-27 [PMID: 20107078]

Bioinformatics

• Gene Symbol: BAMBI
• Entrez:
  • 25805(Human)
• OMIM:
  • 604444(Human)
• Uniprot:
  • AAH19252(Human)