B7-H3/CD276 Antibody

Images

 
Genetic Strategies: Knockout Validated: B7-H3/CD276 Antibody [NBP1-88966] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was ...read more
Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Staining in human placenta and pancreas tissues using anti-CD276 antibody. Corresponding CD276 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: B7-H3/CD276 Antibody [NBP1-88966] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human placenta shows high expression.
Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human pancreas shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

B7-H3/CD276 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EVQVPEDPVVALVGTDATLCCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFG
Specificity
Specificity of human B7-H3/CD276 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD276
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockout Validated 0.5 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Positive Control
B7-H3/CD276 Lysate (NBP2-64854)
B7-H3/CD276 Lysate (NBP2-65934)
Control Peptide
B7-H3/CD276 Protein (NBP1-88966PEP)
Publications
Read Publication using NBP1-88966.

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (89%), Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for B7-H3/CD276 Antibody

  • B7H3
  • B7-H3
  • B7H34Ig-B7-H3
  • B7-H3B7 homolog 3
  • CD276 antigen
  • CD276 molecule
  • CD276
  • Costimulatory molecule

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B7-H3/CD276 Antibody (NBP1-88966)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for B7-H3/CD276 Antibody (NBP1-88966) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for B7-H3/CD276 Antibody (NBP1-88966)

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Diseases for B7-H3/CD276 Antibody (NBP1-88966)

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Bioinformatics

Gene Symbol CD276
Entrez