B7-H3/CD276 Antibodies
We offer B7-H3/CD276 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Neutralization, Simple Western, Western Blot. Each B7-H3/CD276 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our B7-H3/CD276 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the B7-H3/CD276 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our B7-H3/CD276 polyclonal antibodies and browse our B7-H3/CD276 monoclonal antibody catalog.
Alternate Names for B7-H3/CD276 Antibodies
anti-B7-H3/CD276 antibody, anti-CD276 antibody, anti-B7-H3B7 homolog 3 antibody, anti-B7H34Ig-B7-H3 antibody, anti-CD276 antigen antibody, anti-CD276 molecule antibody, anti-Costimulatory molecule antibody
29 Results for "b7-h3-cd276" in Primary Antibodies
![Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88966) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Knockout-Validated-NBP1-88966-img0016.jpg "<b>Genetic Strategies Validation. </b>Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88966) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88966-img0006.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Analysis in control (vector only transfected HEK293T lysate) and CD276 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP1-88966-img0017.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-Paraffin-NBP1-88966-img0014.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human endometrium shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-88966_rabbit-polyclonal-b7-h3-cd276-antibody-immunohistochemistry-paraffin-122202411124..jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] -Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-88966_rabbit-polyclonal-b7-h3-cd276-antibody-immunohistochemistry-paraffin-122202411220..jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] -Staining of human cerebellum shows negative cytoplasmic positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-88966_rabbit-polyclonal-b7-h3-cd276-antibody-immunohistochemistry-paraffin-122202411310..jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP3-14669] - Samples: Whole cell lysate (50 ug) from TCMK-1, NIH 3T3,AlphaTC1 Clone 9, 3T3-L1, and CT26 cells prepared usingNETN lysis buffer. Antibody: Rabbit anti-B7-H3/CD276recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goatanti-rabbit IgG. Chemiluminescence with anexposure time of 3 minutes.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP3-14669-img0002.jpg "Western Blot: B7-H3/CD276 Antibody [NBP3-14669] - Samples: Whole cell lysate (50 ug) from TCMK-1, NIH 3T3,AlphaTC1 Clone 9, 3T3-L1, and CT26 cells prepared usingNETN lysis buffer. Antibody: Rabbit anti-B7-H3/CD276recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goatanti-rabbit IgG. Chemiluminescence with anexposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: B7-H3/CD276 Antibody (BLR025F) [NBP3-14669] - Section of A-172 cells. Antibody: Rabbit anti-B7-H3/CD276 recombinant monoclonal used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-NBP3-14669-img0005.jpg)
![Immunohistochemistry: B7-H3/CD276 Antibody [NBP3-14669] - Sample: FFPE section of humanlung carcinoma. Antibody: Rabbit anti-B7-H3/CD276recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: Opal™. Counterstain:DAPI (blue).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-NBP3-14669-img0006.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP3-14669] - Rabbit anti CD276 recombinant monoclonal antibody (NBP3-14669 lot 1) used at 1:1000. HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH (NB300-322).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP3-14669-img0001.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody (BLR025F) [NBP3-14669] - Section of ovarian carcinoma. Antibody: Rabbit anti-B7-H3/CD276 recombinant monoclonal antibody used at 1:200. Secondary: HRP-conjugated goat antirabbit IgG (A120-501P). Substrate: DAB.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-NBP3-14669-img0004.jpg)
![Immunoprecipitation: B7-H3/CD276 Antibody [NBP3-14669] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-B7-H3/CD276 recombinant monoclonal antibodyused for IP at 3 ul per reaction. B7-H3/CD276 was also immunoprecipitated by rabbit anti-B7-H3/CD276 recombinant monoclonal antibody. For blotting immunoprecipitated B7-H3/CD27 was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunoprecipitation-NBP3-14669-img0003.jpg)
![B7-H3/CD276 Antibody (BLR025F) [NBP3-14669] - Detection of human B7-H3/CD276 (shaded) in 293T cells by flow cytometry. Antibody: Rabbit antiB7-H3/CD276 recombinant monoclonal [BLR025F]or isotype control (unshaded).Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-BLR025F-NBP3-14669-img0010.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88965] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88965) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Knockout-Validated-NBP1-88965-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: B7-H3/CD276 Antibody [NBP1-88965] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88965) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88965] - Staining in human prostate and pancreas tissues using anti-CD276 antibody. Corresponding CD276 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-Paraffin-NBP1-88965-img0013.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88965] - Analysis in control (vector only transfected HEK293T lysate) and CD276 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP1-88965-img0015.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88965] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP1-88965-img0005.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88965] - Staining of human prostate shows high expression.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-Paraffin-NBP1-88965-img0001.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88965] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-Paraffin-NBP1-88965-img0012.jpg)
![Western Blot: B7-H3/CD276 Antibody (6A1) [NBP2-37657] - Western blot analysis using CD276 mAb against HEK293 (1) and CD276(AA: 30-130)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-6A1-Western-Blot-NBP2-37657-img0001.jpg "Western Blot: B7-H3/CD276 Antibody (6A1) [NBP2-37657] - Western blot analysis using CD276 mAb against HEK293 (1) and CD276(AA: 30-130)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody (6A1) [NBP2-37657] - Analysis of cervical cancer tissues (left) and ovarian cancer tissues (right) using CD276 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-6A1-Immunohistochemistry-NBP2-37657-img0003.jpg)
![Flow Cytometry: B7-H3/CD276 Antibody (6A1) [NBP2-37657] - Analysis of PC-3 cells using CD276 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-6A1-Flow-Cytometry-NBP2-37657-img0002.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-B7-H3/CD276 (NBP2-32251). Detection: DAB Counterstain: IHC hematoxylin](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-NBP2-32251-img0002.jpg "Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-B7-H3/CD276 (NBP2-32251). Detection: DAB Counterstain: IHC hematoxylin")
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251] - Samples: FFPE sections of MCF-7 cells (endogenous expression) and CD276 transfected Hek293 cells (over-expression). Antibody: Affinity purified rabbit anti-B7-H3/CD276 (NBP2-32251). Detection: DAB](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunocytochemistry-NBP2-32251-img0003.jpg)
![Immunoprecipitation: B7-H3/CD276 Antibody [NBP2-32251] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-CD276 antibody NBP2-32251 used for IP at 6 ug per reaction. CD276 was also immunoprecipitated by rabbit anti-CD276 antibody BL16383. For blotting immunoprecipitated CD276 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunoprecipitation-NBP2-32251-img0001.jpg)
![Western Blot: B7-H3/CD276 Antibody (PSH02-46) [NBP3-32139] - Western blot analysis of B7-H3/CD276 on different lysates with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: LoVo cell lysate Lane 5: U-2 OS cell lysate Lane 6: LNCaP cell lysate Lane 7: SH-SY5Y cell lysate Lane 8: THP-1 cell lysate Lane 9: HCT 116 cell lysate Lane 10: Raji cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 100 kDa Exposure time: 42 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32139) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32139_rabbit-b7-h3-cd276-mab-psh02-46-278202415212492.jpg "Western Blot: B7-H3/CD276 Antibody (PSH02-46) [NBP3-32139] - Western blot analysis of B7-H3/CD276 on different lysates with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: LoVo cell lysate Lane 5: U-2 OS cell lysate Lane 6: LNCaP cell lysate Lane 7: SH-SY5Y cell lysate Lane 8: THP-1 cell lysate Lane 9: HCT 116 cell lysate Lane 10: Raji cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 100 kDa Exposure time: 42 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32139) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: B7-H3/CD276 Antibody (PSH02-46) [NBP3-32139] - Immunocytochemistry analysis of THP-1 cells labeling B7-H3/CD276 with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32139_rabbit-b7-h3-cd276-mab-psh02-46-2920241048482.jpg)
![Flow Cytometry: B7-H3/CD276 Antibody (PSH02-46) [NBP3-32139] - Flow cytometric analysis of THP-1 cells labeling B7-H3/CD276. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32139, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32139_rabbit-b7-h3-cd276-mab-psh02-46-392024884623.jpg)