| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1027-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB1027A | |||
| Carrier Free | FAB1027F | |||
| Carrier Free | FAB1027P | |||
| Carrier Free | MAB1027-SP | |||
| Carrier Free | MAB1027-500 | |||
| Carrier Free | FAB1027V-025 | |||
| Carrier Free | FAB1027U-100UG | |||
| Carrier Free | FAB1027S-100UG | |||
| Carrier Free | FAB1027R-100UG | |||
| Carrier Free | FAB1027G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88966-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 1397-B3-050/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1397-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | PDB7H30 | |||
| Carrier Free | SB7H30 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88965-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 1949-B3-050/CF | |||
![Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88966) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Knockout-Validated-NBP1-88966-img0016.jpg "<b>Genetic Strategies Validation. </b>Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88966) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88966-img0006.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88966] - Analysis in control (vector only transfected HEK293T lysate) and CD276 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP1-88966-img0017.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-Paraffin-NBP1-88966-img0014.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] - Staining of human endometrium shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-88966_rabbit-polyclonal-b7-h3-cd276-antibody-immunohistochemistry-paraffin-122202411124..jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] -Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-88966_rabbit-polyclonal-b7-h3-cd276-antibody-immunohistochemistry-paraffin-122202411220..jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88966] -Staining of human cerebellum shows negative cytoplasmic positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-88966_rabbit-polyclonal-b7-h3-cd276-antibody-immunohistochemistry-paraffin-122202411310..jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP3-14669] - Samples: Whole cell lysate (50 ug) from TCMK-1, NIH 3T3,AlphaTC1 Clone 9, 3T3-L1, and CT26 cells prepared usingNETN lysis buffer. Antibody: Rabbit anti-B7-H3/CD276recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goatanti-rabbit IgG. Chemiluminescence with anexposure time of 3 minutes.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP3-14669-img0002.jpg "Western Blot: B7-H3/CD276 Antibody [NBP3-14669] - Samples: Whole cell lysate (50 ug) from TCMK-1, NIH 3T3,AlphaTC1 Clone 9, 3T3-L1, and CT26 cells prepared usingNETN lysis buffer. Antibody: Rabbit anti-B7-H3/CD276recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goatanti-rabbit IgG. Chemiluminescence with anexposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: B7-H3/CD276 Antibody (BLR025F) [NBP3-14669] - Section of A-172 cells. Antibody: Rabbit anti-B7-H3/CD276 recombinant monoclonal used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-NBP3-14669-img0005.jpg)
![Immunohistochemistry: B7-H3/CD276 Antibody [NBP3-14669] - Sample: FFPE section of humanlung carcinoma. Antibody: Rabbit anti-B7-H3/CD276recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: Opal™. Counterstain:DAPI (blue).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-NBP3-14669-img0006.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP3-14669] - Rabbit anti CD276 recombinant monoclonal antibody (NBP3-14669 lot 1) used at 1:1000. HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH (NB300-322).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP3-14669-img0001.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody (BLR025F) [NBP3-14669] - Section of ovarian carcinoma. Antibody: Rabbit anti-B7-H3/CD276 recombinant monoclonal antibody used at 1:200. Secondary: HRP-conjugated goat antirabbit IgG (A120-501P). Substrate: DAB.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-NBP3-14669-img0004.jpg)
![Immunoprecipitation: B7-H3/CD276 Antibody [NBP3-14669] - Samples: Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-B7-H3/CD276 recombinant monoclonal antibodyused for IP at 3 ul per reaction. B7-H3/CD276 was also immunoprecipitated by rabbit anti-B7-H3/CD276 recombinant monoclonal antibody. For blotting immunoprecipitated B7-H3/CD27 was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunoprecipitation-NBP3-14669-img0003.jpg)
![B7-H3/CD276 Antibody (BLR025F) [NBP3-14669] - Detection of human B7-H3/CD276 (shaded) in 293T cells by flow cytometry. Antibody: Rabbit antiB7-H3/CD276 recombinant monoclonal [BLR025F]or isotype control (unshaded).Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-BLR025F-NBP3-14669-img0010.jpg)
![N/A B7-H3/CD276 [HRP]](http://images.novusbio.com/fullsize2/DATA_B7H3_DB7H30_ELISA_583.jpg "N/A B7-H3/CD276 [HRP]")
![N/A B7-H3/CD276 [HRP]](http://images.novusbio.com/fullsize2/B7-H3_DB7H30_ELISA_34.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88965] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88965) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Knockout-Validated-NBP1-88965-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: B7-H3/CD276 Antibody [NBP1-88965] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88965) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88965] - Staining in human prostate and pancreas tissues using anti-CD276 antibody. Corresponding CD276 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-Paraffin-NBP1-88965-img0013.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88965] - Analysis in control (vector only transfected HEK293T lysate) and CD276 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP1-88965-img0015.jpg)
![Western Blot: B7-H3/CD276 Antibody [NBP1-88965] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Western-Blot-NBP1-88965-img0005.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88965] - Staining of human prostate shows high expression.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-Paraffin-NBP1-88965-img0001.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88965] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-Paraffin-NBP1-88965-img0012.jpg)