B7-H2/ICOSLG Antibody (HK5.3) [Biotin]

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Flow Cytometry: B7-H2/ICOSLG Antibody (HK5.3) [Biotin] [NBP1-43505] - Staining of mouse splenocytes with Rat IgG2a Isotype Control Biotin (open histogram) or Anti-Mouse CD275 (B7-H2) Biotin (filled histogram) followed ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
HK5.3
Clonality
Monoclonal
Host
Rat
Conjugate
Biotin
Concentration
0.5 mg/ml

Order Details

B7-H2/ICOSLG Antibody (HK5.3) [Biotin] Summary

Immunogen
The immunogen for this antibody was B7RP1.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
ICOSLG
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The HK5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Readout System

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for B7-H2/ICOSLG Antibody (HK5.3) [Biotin]

  • B7H2
  • B7-H2
  • B7H2B7 homolog 2
  • B7-H2B7-related protein 1
  • B7RP-1
  • B7RP1B7-like protein Gl50
  • B7RP-1LICOS
  • CD275 antigen
  • CD275
  • GL50
  • GL50transmembrane protein B7-H2 ICOS ligand
  • ICOSL
  • ICOS-L
  • ICOSLG
  • inducible T-cell co-stimulator ligand
  • KIAA0653ICOS ligand

Background

The HK5.3 monoclonal antibody reacts with mouse B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of ~40 kDa and belongs to the Ig superfamily. Mouse B7RP-1 is expressed by antigen presenting cells including B cells, monocytes/macrophages and dendritic cells. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ICOSLG
Entrez