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MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B)." class="big_lightbox" target="_blank">
MAB100), Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) stimulates proliferation in PHA-activated human T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human B7-H2 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7-H2 Monoclonal Antibody (Catalog # MAB165). The ND50 is typically 1-4 µg/mL." class="big_lightbox" target="_blank">
HAF017). A specific band was detected for B7-H2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox" target="_blank">
MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B)." class="big_lightbox" target="_blank">
169-CS) is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human B7-H2 Fc Chimera Avi-tag (AVI165) that produces a 50% optimal binding response is found to be 1-6 ng/mL." class="big_lightbox" target="_blank">
AVI165) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-100 kDa and 160-200 kDa, respectively." class="big_lightbox" target="_blank">
B7-RP1 is a 40 kD protein, also known as ICOS ligand, B7-H2, and B7h. It has recently been clustered as CD275. It is a member of the immunoglobulin receptor superfamily expressed on splenic B cells, dendritic cells and macrophages. The expression of this antigen is increased by TNF- alpha stimulation and is highly upregulated by local inflammation. B7RP1 binds to ICOS and co-stimulates T cell and B cell responses. Two isoforms of this protein have been reported to occur as a result of alternative splicing. These isoforms show differential tissue distribution.