B7-H2/ICOSLG Antibodies

MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_lightbox"> MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." alt="U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human B7‑H2 Monoclonal Antibody (Catalog # MAB165, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

MAB100), Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) stimulates proliferation in PHA-activated human T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human B7-H2 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7-H2 Monoclonal Antibody (Catalog # MAB165). The ND₅₀ is typically 1-4 µg/mL." class="big_lightbox"> MAB100), Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) stimulates proliferation in PHA-activated human T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human B7-H2 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7-H2 Monoclonal Antibody (Catalog # MAB165). The ND₅₀ is typically 1-4 µg/mL." class="big_thumb" />

HAF017). A specific band was detected for B7-H2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for B7-H2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human B7-H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF165) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for B7-H2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox"> AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />

MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B)." class="big_lightbox"> MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B)." alt="A20 mouse B cell lymphoma cell line was stained with Mouse B7-H2 Monoclonal Antibody (Catalog # MAB158, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B)." class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

168-CS, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant mouse B7-H2 (black dotted line). Binding is completely blocked (orange histogram) by 0.5 µg/mL of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083). Rat IgG2A Isotype Control (Catalog # MAB006) at 0.5 µg/mL was used as a control (blue line). Ligand binding was detected by staining cells with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A)." class="big_lightbox"> 168-CS, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant mouse B7-H2 (black dotted line). Binding is completely blocked (orange histogram) by 0.5 µg/mL of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083). Rat IgG2A Isotype Control (Catalog # MAB006) at 0.5 µg/mL was used as a control (blue line). Ligand binding was detected by staining cells with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A)." alt="In a functional flow cytometry test, Recombinant Mouse ICOS Fc Chimera (Catalog # 168-CS, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant mouse B7-H2 (black dotted line). Binding is completely blocked (orange histogram) by 0.5 µg/mL of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083). Rat IgG2A Isotype Control (Catalog # MAB006) at 0.5 µg/mL was used as a control (blue line). Ligand binding was detected by staining cells with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A)." class="big_thumb" />

F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

8127-B7) induces IL-4 secretion in the D10.G4.1 mouse helper T cell line in the presence of Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) in a dose-dependent manner (orange line), as measured by the Mouse IL-4 Quantikine ELISA Kit (Catalog # M4000B). Under these conditions, IL-4 secretion elicited by B7-H2 (2.5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083). The ND₅₀ is typically 0.3-1.8 µg/mL." class="big_lightbox"> 8127-B7) induces IL-4 secretion in the D10.G4.1 mouse helper T cell line in the presence of Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) in a dose-dependent manner (orange line), as measured by the Mouse IL-4 Quantikine ELISA Kit (Catalog # M4000B). Under these conditions, IL-4 secretion elicited by B7-H2 (2.5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083). The ND₅₀ is typically 0.3-1.8 µg/mL." class="big_thumb" />

F0113). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0113). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with either (A) mouse B7-H2 or (B) irrelevant transfectants and eGFP were stained with Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB1582) followed by Goat Anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

168-CS; 50 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with mouse B7-H2. Binding is blocked by (A) 2.5 µg/mL of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB1582) but not by (B) Rat IgG1 Isotype Control (MAB005) at 2.5 µg/mL. Protein binding was detected with APC-conjugated anti-Human IgG Fc Monoclonal Antibody (FAB110A). " class="big_lightbox"> 168-CS; 50 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with mouse B7-H2. Binding is blocked by (A) 2.5 µg/mL of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB1582) but not by (B) Rat IgG1 Isotype Control (MAB005) at 2.5 µg/mL. Protein binding was detected with APC-conjugated anti-Human IgG Fc Monoclonal Antibody (FAB110A). " class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="B7‑H2 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse B7‑H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF158) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

MAB100), Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) stimulates proliferation in PHA-activated human T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Rec-ombinant Human B7-H2 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7-H2 Monoclonal Antibody (Catalog # MAB1651). The ND₅₀ is typically 1-4 µg/mL." class="big_lightbox"> MAB100), Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) stimulates proliferation in PHA-activated human T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Rec-ombinant Human B7-H2 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7-H2 Monoclonal Antibody (Catalog # MAB1651). The ND₅₀ is typically 1-4 µg/mL." alt="In the presence of Human CD3e Monoclonal Antibody (20 ng/mL, Catalog # MAB100), Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) stimulates proliferation in PHA-activated human T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Rec-ombinant Human B7-H2 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7-H2 Monoclonal Antibody (Catalog # MAB1651). The ND₅₀ is typically 1-4 µg/mL." class="big_thumb" />