B7-1/CD80 Antibody (37711) [Fluorescein]

Human PBMC were treated with 1μg/mL LPS overnight then stained with (A) Mouse Anti-Human B7-1/CD80 CFS-conjugated Monoclonal Antibody (Catalog # FAB140F) or (B) Mouse IgG1 Isotype Control (IC002F) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7‑1/CD80 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB140F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 37711
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

B7-1/CD80 Antibody (37711) [Fluorescein] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑1/CD80
  Val35-Asn242 (predicted)
  Accession # P33681
• Specificity: Detects human B7‑1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD80
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-1/CD80 Antibody (37711) [Fluorescein]

• Activation B7-1 antigen
• B7
• B71
• B7-1
• BB1
• CD28LG1
• CD28LGB7-1 antigen)
• CD80 antigen
• CD80 molecule
• CD80
• costimulatory factor CD80
• costimulatory molecule variant IgV-CD80
• CTLA-4 counter-receptor B7.1
• T-lymphocyte activation antigen CD80

Background

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through Interferon gamma . B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% aa sequence identity. Human and mouse B7-1 share 44% aa sequence identity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

1. Azuma, M. et al. (1993) Nature 366:76.
2. Freeman, G.J. et al. (1993) Science 262:909.
3. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
4. Selvakumar, A. et al. (1993) Immunogenetics 38:292.
5. Chen, C. et al. (1994) J. Immunol. 152:4929.
6. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B7-1/CD80 Antibody (FAB140F)(6)

Publications using FAB140F

• Beliakova-Bethell N, Manousopoulou A, Deshmukh S et al. Integrated proteomics and transcriptomics analyses identify novel cell surface markers of HIV latency Virology 6/4/2022 [PMID: 35714458]
• Subramanya N. Pandruvada, Jeffrey L. Ebersole, Sarandeep S. Huja Inhibition of osteoclastogenesis by opsonized Porphyromonas gingivalis FASEB BioAdvances 7/30/2019 [PMID: 31355360]
• KP Bene, DW Kavanaugh, C Leclaire, AP Gunning, DA MacKenzie, A Wittmann, ID Young, N Kawasaki, E Rajnavolgy, N Juge Lactobacillus reuteri Surface Mucus Adhesins Upregulate Inflammatory Responses Through Interactions With Innate C-Type Lectin Receptors Front Microbiol, 2017-03-07;8(0):321. 2017-03-07 [PMID: 28326063] (Flow Cytometry, Human)
• Petterson T, Jendholm J, Mansson A, Bjartell A, Riesbeck K, Cardell LO Effects of NOD-like receptors in human B lymphocytes and crosstalk between NOD1/NOD2 and Toll-like receptors. J. Leukoc. Biol., 2010-09-15;89(2):177-87. 2010-09-15 [PMID: 20844241] (Flow Cytometry, Human)
• Kraetzel K, Stoelcker B, Eissner G, Multhoff G, Pfeifer M, Holler E, Schulz C NKG2D-dependent effector function of bronchial epithelium-activated alloreactive T-cells. Eur. Respir. J., 2008-04-16;32(3):563-70. 2008-04-16 [PMID: 18417514] (Flow Cytometry, Human)
• Kaur K, Chowdhury S, Greenspan NS, Schreiber JR Decreased expression of tumor necrosis factor family receptors involved in humoral immune responses in preterm neonates. Blood, 2007-07-18;110(8):2948-54. 2007-07-18 [PMID: 17634409] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CD80
• Uniprot:
  • P33681()