B7-1/CD80 Antibody (1G10) [Biotin]

Flow Cytometry: B7-1/CD80 Antibody (1G10) [Biotin] [NBP1-28028] - DBA/2 mouse mastocytoma cell line P815 and mB7.1-Ig transfected P815 cells were stained with Rat Anti-Mouse CD80-BIOT followed by Streptavidin-FITC.

Product Discontinued

B7-1/CD80 Antibody (1G10) [Biotin] Summary

• Immunogen: Activated 5C2 cells
• Specificity: Mouse CD80 (B7-1), Mr 45-47 kDa
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: CD80
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for B7-1/CD80 Antibody (1G10) [Biotin]

• Activation B7-1 antigen
• B7
• B71
• B7-1
• BB1
• CD28LG1
• CD28LGB7-1 antigen)
• CD80 antigen
• CD80 molecule
• CD80
• costimulatory factor CD80
• costimulatory molecule variant IgV-CD80
• CTLA-4 counter-receptor B7.1
• T-lymphocyte activation antigen CD80

Background

CD80 (B7-1) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed by dendritic cells, monocytes, and peritoneal macrophages. Expression of CD80 on B cells is upregulated after activation by various means including stimulation by LPS, IL-4, and surface Ig receptor cross linking with peak expression occurring between 48 and 72 hours postactivation. (1-4) The interaction of CD80/B7-1 with its ligands, CD28 and CD152/CTLA-4, provides a potent costimulatory signal for T cells activated through the CD3/T cell receptor complex. (5, 6) The monoclonal antibody 1G10 blocks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigen presenting cells. (2, 7)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD80