B7-1/CD80 Antibody (16-10A1)

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the PE/Cy5 conjugate of NBP1-43385. Staining of 3-day LPS activated C57Bl/6 splenocytes with 0.03 ug of PE-Cy5 Armenian Hamster IgG Isotype Control (open histogram) or 0.03 ug of Anti-Mouse CD80 (B7-1) PE-Cy5 (filled histogram). Total cells

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Staining of 3-day LPS-stimulated BALB/c splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD80 (B7-1) Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC. Total viable cells were used for analysis.

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the Biotin conjugate of NBP1-43385. Staining of 3-day LPS-stimulated C57BL/6 splenocytes with 0.06 ug of Armenian Hamster IgG Isotype Control Biotin (open histogram) or 0.06 ug of Anti-Mouse CD80 (B7-1) Biotin (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the FITC conjugate of NBP1-43385. Staining of unstimulated (left) or 3-day LPS-stimulated (right) C57BL/6 splenocytes with 0.125 ug of Anti-Mouse CD80 (B7-1) FITC. Total viable cells were used for analysis. Markers were set based on the autofluorescence sample.

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [NBP1-43385] - Analysis using the PE conjugate of NBP1-43385. See Notes

• Reactivity: Hu, Mu
• Applications: Flow, IHC, IHC-Fr, IP
• Clone: 16-10A1
• Clonality: Monoclonal
• Host: Armenian Hamster
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

B7-1/CD80 Antibody (16-10A1) Summary

• Immunogen: The immunogen for this antibody was CD80.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: CD80
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for B7-1/CD80 Antibody (16-10A1)

• Activation B7-1 antigen
• B7
• B71
• B7-1
• BB1
• CD28LG1
• CD28LGB7-1 antigen)
• CD80 antigen
• CD80 molecule
• CD80
• costimulatory factor CD80
• costimulatory molecule variant IgV-CD80
• CTLA-4 counter-receptor B7.1
• T-lymphocyte activation antigen CD80

Background

The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for B7-1/CD80 Antibody (NBP1-43385). (Showing 1 - 1 of 1 FAQ).

1. Does this antibody stain the membrane or the cytoplasm of macrophages?
   • Please note that CD80 is a transmembrane protein with extracellular, helical and cytoplasmic domains. Accordingly, in immunostaining assays, you can expect staining at and around the cellular membranes.

Bioinformatics

• Gene Symbol: CD80