B7-1/CD80 Antibody (16-10A1) [PE/Cy5]

Flow Cytometry: B7-1/CD80 Antibody (16-10A1) [PE/Cy5] [NBP1-43086] - Staining of 3-day LPS activated C57Bl/6 splenocytes with 0.03 ug of PE-Cy5 Armenian Hamster IgG Isotype Control (open histogram) or 0.03 ug of Anti-Mouse CD80 (B7-1) PE-Cy5 (filled histogram). Total cells were used for analysis.

• Reactivity: Hu, Mu, Fe
• Applications: Flow
• Clone: 16-10A1
• Clonality: Monoclonal
• Host: Armenian Hamster
• Conjugate: PE/Cy5
• Concentration: 0.2 mg/ml

B7-1/CD80 Antibody (16-10A1) [PE/Cy5] Summary

• Immunogen: The immunogen for this antibody was CD80.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: CD80
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

Feline reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2) with 0.1% gelatin
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for B7-1/CD80 Antibody (16-10A1) [PE/Cy5]

• Activation B7-1 antigen
• B7
• B71
• B7-1
• BB1
• CD28LG1
• CD28LGB7-1 antigen)
• CD80 antigen
• CD80 molecule
• CD80
• costimulatory factor CD80
• costimulatory molecule variant IgV-CD80
• CTLA-4 counter-receptor B7.1
• T-lymphocyte activation antigen CD80

Background

The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD80