SNRPE Antibody

Western Blot: SNRPE Antibody [NBP2-43792] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SNRPE antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

SNRPE antibody detects SNRPE protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.SNRPE stained by SNRPE antibody (NBP2-43792) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SNRPE antibody detects SNRPE protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.SNRPE stained by SNRPE antibody (NBP2-43792) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SNRPE Antibody Summary

• Description: Novus Biologicals Rabbit SNRPE Antibody (NBP2-43792) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-SNRPE Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SNRPE. The exact sequence is proprietary.
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%), Rhesus Macaque (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SNRPE
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID:34096600)
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SNRPE Antibody

• B-raf
• Sm protein E
• small nuclear ribonucleoprotein E
• small nuclear ribonucleoprotein polypeptide E
• SmE
• Sm-ESME
• snRNP-E

Background

Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SNRPE Antibody (NBP2-43792)(2)

Publications using NBP2-43792

• Esser LM, Schmitz K, Hillebrand F et al. Phosphorylation of pICln by the autophagy activating kinase ULK1 regulates snRNP biogenesis and splice activity of the cell Computational and structural biotechnology journal 2023-03-16 [PMID: 36968021] (WB, ICC/IF, Human)
• Schmitz K, Cox J, Esser LM et al. An essential role of the autophagy activating kinase ULK1 in snRNP biogenesis Nucleic acids research 2021-06-21 [PMID: 34096600] (ICC/IF, WB, Human)

Bioinformatics

• Gene Symbol: SNRPE