B-Raf: Novus Biologicals

NBP1-89942

B-Raf Antibody - BSA Free

(1 Publication)

0.1 ml

NBP1-89942

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human kidney shows moderate cytoplasmic positivity in distal tubules.

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Western Blot: B-Raf Antibody [NBP1-89942] - Highly efficient wild-type down-regulation & concomitant mutant complementation for BRAF, HRAS & SHP2 proteins by TEMTAC.(a) Expression & phenotype analysis of BRAF V600E & WT in HEK293 cells is shown. Left panel: Western blot results using specific antibodies as indicated. Right panel: MEKp quantification after intensity analysis using ImageJ. (b) Expression & phenotype analysis of HRAS G12V & WT in HEK293 cells. Left panel: Western blot results using antibodies as indicated. Right panel: MEKp quantification after intensity analysis using ImageJ. (c) Expression & phenotype analysis of SHP2 C459G, D61G & WT in GH-HEK293 cells (HEK293 cells stably expressing the growth hormone receptor). Left panel: Western blot results using antibodies as indicated. Right panel: ERKp quantification after intensity analysis using ImageJ. The dashed line indicates that the blot has been cropped. The full blot is provided in Supplementary Figure S11. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26612112), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Simple Western: B-Raf Antibody [NBP1-89942] - Simple Western lane view shows a specific band for BRAF in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: B-Raf Antibody [NBP1-89942] - Electropherogram image(s) of corresponding Simple Western lane view. B-Raf antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-47668

B-Raf Antibody (OTI5A9)

(1 Publication)

0.1 ml

NBP1-47668

Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Human pancreas tissue using anti-B Raf mouse monoclonal antibody.

Flow Cytometry: B-Raf Antibody (OTI5A9) [NBP1-47668] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-B Raf antibody, and then analyzed by flow cytometry.

Western Blot: B-Raf Antibody (OTI5A9) [NBP1-47668] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antibody

Immunohistochemistry: B-Raf Antibody (OTI5A9) [NBP1-47668] - The images depict immunostaining of representative PTC cases harboring BRAF wild-type (A, B) and BRAF V600E mutation (C, D) proven by NGS; varying degrees of immunoreactivity for BRAF is shown both in the intranuclear inclusions (arrows) and in the cytoplasm. Original magnifications: 1,000 X.

Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-B Raf mouse monoclonal antibody.

Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-B Raf mouse monoclonal antibody.

Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Human endometrium tissue using anti-B Raf mouse monoclonal antibody.

Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Human lung tissue using anti-B Raf mouse monoclonal antibody.

Immunoprecipitation: B-Raf Antibody (OTI5A9) [NBP1-47668] - (Negative control: IP without adding anti-BRAF antibody.). For each experiment, 500ul of DDK tagged BRAF overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-BRAF antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70260AF350
Alexa Fluor 405	NBP2-70260AF405
Alexa Fluor 488	NBP2-70260AF488
Alexa Fluor 532	NBP2-70260AF532
Alexa Fluor 594	NBP2-70260AF594
Alexa Fluor 647	NBP2-70260AF647
Alexa Fluor 700	NBP2-70260AF700
Alexa Fluor 750	NBP2-70260AF750
Alexa Fluor Plus 405	NBP2-70260AFP405
Alexa Fluor Plus 488	NBP2-70260AFP488
Alexa Fluor Plus 555	NBP2-70260AFP555
Alexa Fluor Plus 594	NBP2-70260AFP594
Alexa Fluor Plus 647	NBP2-70260AFP647
Alexa Fluor Plus 680	NBP2-70260AFP680
Allophycocyanin	NBP2-70260APC
Biotin	NBP2-70260B
CoraFluor 1	NBP2-70260CL1
DyLight 350	NBP2-70260UV
DyLight 405	NBP2-70260V
DyLight 488	NBP2-70260G
DyLight 550	NBP2-70260R
DyLight 594	NBP2-70260DL594
DyLight 650	NBP2-70260C
DyLight 680	NBP2-70260FR
DyLight 755	NBP2-70260IR
FITC	NBP2-70260F
HRP	NBP2-70260H
Janelia Fluor 525	NBP2-70260JF525
Janelia Fluor 549	NBP2-70260JF549
Janelia Fluor 646	NBP2-70260JF646
Janelia Fluor 669	NBP2-70260JF669
PE	NBP2-70260PE
PerCP	NBP2-70260PCP
mFluor Violet 450 SE	NBP2-70260MFV450
mFluor Violet 500 SE	NBP2-70260MFV500
mFluor Violet 610 SE	NBP2-70260MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70260

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AF3424

HAF109). A specific band was detected for B-Raf at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of T47D human breast cancer cell line, MDA-MB-453 human breast cancer cell line, Daudi human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line and U266 human myeloma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B-Raf Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3424) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). A specific band was detected for B-Raf at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of T47D human breast cancer cell line, MDA-MB-453 human breast cancer cell line, Daudi human Burkitt's lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line and U266 human myeloma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B-Raf Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3424) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for B-Raf at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for B-Raf at approximately 95 kDa (as indicated) using 10 µg/mL of Goat Anti-Human B-Raf Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3424) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for B‑Raf at approximately 95 kDa (as indicated) using 10 µg/mL of Goat Anti-Human B‑Raf Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3424) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

$Changes in ERK1/2 and MEK in response to PLX4032. (A) Melanoma cell strains were treated with DMSO for 4 h (0), or with PLX4032 (1 μM) for 4 and 8 h. The panels show Western blots probed with antibodies to phosph-ERK1/2 Thr202/Tyr204 mAb (pERK), ERK1/2 (ERK), phospho-MEK1/2 (pMEK), MEK1/2 (MEK), and actin as a loading control. The mutation status of BRAF, NRAS, PTEN and beta -catenin ( beta CAT) are indicated at the top. (B) Western blot analyses of ERK1/2 inactivation/activation after short-term incubation with PLX4032 (1 μM). (C) pERK1/2 and ERK1/2 in supernatant (Sup) and particulate (Part) fractions. (D) Changes in pERK1/2 activation after treatments with increasing concentration of PLX4032, or DMSO for 1 h. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/20149136), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3424U-100UG
Alexa Fluor 405	AF3424V-100UG
Alexa Fluor 488	AF3424G-100UG
Alexa Fluor 532	AF3424X-100UG
Alexa Fluor 594	AF3424T-100UG
Alexa Fluor 647	AF3424R-100UG
Alexa Fluor 700	AF3424N-100UG
Alexa Fluor 750	AF3424S-100UG
Alexa Fluor Plus 405	AF3424AFP405-100UG
Alexa Fluor Plus 488	AF3424AFP488-100UG
Alexa Fluor Plus 555	AF3424AFP555-100UG
Alexa Fluor Plus 594	AF3424AFP594-100UG
Alexa Fluor Plus 647	AF3424AFP647-100UG
Alexa Fluor Plus 680	AF3424AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3424-SP

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NBP2-61503

B-Raf Antibody (RM8) - V600-E Mutation

100 μg

NBP2-61503

Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Staining of formalin fixed and paraffin embedded human cell line sections (SW40 cells at 400X, negative)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-15531

B-Raf Antibody

0.1 ml

NBP2-15531

Western Blot: B-Raf Antibody [NBP2-15531] - Non-transfected (-) and B-Raf-transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal diluted at 1:5000.

Immunoprecipitation: B-Raf Antibody [NBP2-15531] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of B-Raf protein expressing HepG2 cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of B-Raf by 3 ug of B-Raf antibody [N2C1], Internal 10% SDS-PAGE The immunoprecipitated B-Raf protein was detected by B-Raf antibody [N2C1], Internal diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP2-15531] - B-Raf antibody [N2C1], Internal detects B-Raf protein at cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded human breast carcinoma.</br>B-Raf stained by B-Raf antibody [N2C1], Internal (NBP2-15531) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: B-Raf Antibody [NBP2-15531] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (NBP2-15531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-00517

B-Raf Antibody (IHC600) - V600-E Mutation

0.1 ml

NBP3-00517

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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MAB3424

B-Raf Antibody (336016) [Unconjugated]

100 μg

MAB3424

HAF007). For additional reference, Recombinant Human Active Raf-1 (Y340E Y341E), aa 306-648 (Catalog # 4540-KS) and recombinant human B-Raf (5 ng/lane) were included. A specific band for B-Raf was detected at approximately 95 kDa (as indicated). An unidentified band at 55 kDa was also detected. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox">

Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat B-Raf Monoclonal Antibody (Catalog # MAB3424) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). For additional reference, Recombinant Human Active Raf-1 (Y340E Y341E), aa 306-648 (Catalog # 4540-KS) and recombinant human B-Raf (5 ng/lane) were included. A specific band for B-Raf was detected at approximately 95 kDa (as indicated). An unidentified band at 55 kDa was also detected. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat B-Raf Monoclonal Antibody (Catalog # MAB3424) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Active Raf-1 (Y340E Y341E), aa 306-648 (Catalog # 4540-KS) and recombinant human B-Raf (5 ng/lane) were included. A specific band for B-Raf was detected at approximately 95 kDa (as indicated). An unidentified band at 55 kDa was also detected. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3424U-100UG
Alexa Fluor 405	FAB3424V-100UG
Alexa Fluor 488	FAB3424G-100UG
Alexa Fluor 532	FAB3424X-100UG
Alexa Fluor 594	FAB3424T-100UG
Alexa Fluor 647	FAB3424R-100UG
Alexa Fluor 700	FAB3424N-100UG
Alexa Fluor 750	FAB3424S-100UG
Alexa Fluor Plus 405	FAB3424AFP405-100UG
Alexa Fluor Plus 488	FAB3424AFP488-100UG
Alexa Fluor Plus 555	FAB3424AFP555-100UG
Alexa Fluor Plus 594	FAB3424AFP594-100UG
Alexa Fluor Plus 647	FAB3424AFP647-100UG
Alexa Fluor Plus 680	FAB3424AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3424-SP

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NBP2-59035

B-Raf Antibody (CL4004) - BSA Free

100 ul

NBP2-59035

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035] - Staining of human prostate cancer shows moderate cytoplasmic immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035] - Staining of human breast cancer shows moderate cytoplasmic immunoreactivity in tumor cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44118AF350
Alexa Fluor 405	NBP3-44118AF405
Alexa Fluor 488	NBP3-44118AF488
Alexa Fluor 532	NBP3-44118AF532
Alexa Fluor 594	NBP3-44118AF594
Alexa Fluor 647	NBP3-44118AF647
Alexa Fluor 700	NBP3-44118AF700
Alexa Fluor 750	NBP3-44118AF750
Alexa Fluor Plus 405	NBP3-44118AFP405
Alexa Fluor Plus 488	NBP3-44118AFP488
Alexa Fluor Plus 555	NBP3-44118AFP555
Alexa Fluor Plus 594	NBP3-44118AFP594
Alexa Fluor Plus 647	NBP3-44118AFP647
Alexa Fluor Plus 680	NBP3-44118AFP680
Allophycocyanin	NBP3-44118APC
Biotin	NBP3-44118B
CoraFluor 1	NBP3-44118CL1
DyLight 350	NBP3-44118UV
DyLight 405	NBP3-44118V
DyLight 488	NBP3-44118G
DyLight 550	NBP3-44118R
DyLight 594	NBP3-44118DL594
DyLight 650	NBP3-44118C
DyLight 680	NBP3-44118FR
DyLight 755	NBP3-44118IR
FITC	NBP3-44118F
HRP	NBP3-44118H
Janelia Fluor 525	NBP3-44118JF525
Janelia Fluor 549	NBP3-44118JF549
Janelia Fluor 646	NBP3-44118JF646
Janelia Fluor 669	NBP3-44118JF669
PE	NBP3-44118PE
PerCP	NBP3-44118PCP
mFluor Violet 450 SE	NBP3-44118MFV450
mFluor Violet 500 SE	NBP3-44118MFV500
mFluor Violet 610 SE	NBP3-44118MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44118
BSA Free	NBP2-59035-25ul

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NBP2-59034

B-Raf Antibody (CL4003) - BSA Free

100 ul

NBP2-59034

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4003) [NBP2-59034] - Staining of human testis shows strong cytoplasmic immunoreactivity in seminiferous tubules.

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4003) [NBP2-59034] - Staining of human cerebral cortex shows moderate positivity in neuropil and cytoplasmic staining in some neurons.

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4003) [NBP2-59034] - Staining of human fallopian tube shows strong cytoplasmic immunoreactivity in glandular cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44117AF350
Alexa Fluor 405	NBP3-44117AF405
Alexa Fluor 488	NBP3-44117AF488
Alexa Fluor 532	NBP3-44117AF532
Alexa Fluor 594	NBP3-44117AF594
Alexa Fluor 647	NBP3-44117AF647
Alexa Fluor 700	NBP3-44117AF700
Alexa Fluor 750	NBP3-44117AF750
Alexa Fluor Plus 405	NBP3-44117AFP405
Alexa Fluor Plus 488	NBP3-44117AFP488
Alexa Fluor Plus 555	NBP3-44117AFP555
Alexa Fluor Plus 594	NBP3-44117AFP594
Alexa Fluor Plus 647	NBP3-44117AFP647
Alexa Fluor Plus 680	NBP3-44117AFP680
Allophycocyanin	NBP3-44117APC
Biotin	NBP3-44117B
CoraFluor 1	NBP3-44117CL1
DyLight 350	NBP3-44117UV
DyLight 405	NBP3-44117V
DyLight 488	NBP3-44117G
DyLight 550	NBP3-44117R
DyLight 594	NBP3-44117DL594
DyLight 650	NBP3-44117C
DyLight 680	NBP3-44117FR
DyLight 755	NBP3-44117IR
FITC	NBP3-44117F
HRP	NBP3-44117H
Janelia Fluor 525	NBP3-44117JF525
Janelia Fluor 549	NBP3-44117JF549
Janelia Fluor 646	NBP3-44117JF646
Janelia Fluor 669	NBP3-44117JF669
PE	NBP3-44117PE
PerCP	NBP3-44117PCP
mFluor Violet 450 SE	NBP3-44117MFV450
mFluor Violet 500 SE	NBP3-44117MFV500
mFluor Violet 610 SE	NBP3-44117MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44117
BSA Free	NBP2-59034-25ul

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NBP2-41219

B-Raf Antibody - BSA Free

0.1 mg

NBP2-41219

Western Blot: B-Raf Antibody [NBP2-41219] - Analysis of B-raf in human brain tissue lysate with B-raf antibody at (A) 1 and (B) 2 ug/mL.

Immunocytochemistry/ Immunofluorescence: B-Raf Antibody - BSA Free [NBP2-41219] - Validation of B-Raf in Human Small Intestine TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed Human Small Intestine Tissue labeling B-Raf with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Reactivity:

Hu, Mu, Rt, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Ch

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-41219AF350
Alexa Fluor 405	NBP2-41219AF405
Alexa Fluor 488	NBP2-41219AF488
Alexa Fluor 532	NBP2-41219AF532
Alexa Fluor 594	NBP2-41219AF594
Alexa Fluor 647	NBP2-41219AF647
Alexa Fluor 700	NBP2-41219AF700
Alexa Fluor 750	NBP2-41219AF750
Alexa Fluor Plus 405	NBP2-41219AFP405
Alexa Fluor Plus 488	NBP2-41219AFP488
Alexa Fluor Plus 555	NBP2-41219AFP555
Alexa Fluor Plus 594	NBP2-41219AFP594
Alexa Fluor Plus 647	NBP2-41219AFP647
Alexa Fluor Plus 680	NBP2-41219AFP680
Allophycocyanin	NBP2-41219APC
Biotin	NBP2-41219B
CoraFluor 1	NBP2-41219CL1
DyLight 350	NBP2-41219UV
DyLight 405	NBP2-41219V
DyLight 488	NBP2-41219G
DyLight 550	NBP2-41219R
DyLight 594	NBP2-41219DL594
DyLight 650	NBP2-41219C
DyLight 680	NBP2-41219FR
DyLight 755	NBP2-41219IR
FITC	NBP2-41219F
HRP	NBP2-41219H
Janelia Fluor 525	NBP2-41219JF525
Janelia Fluor 549	NBP2-41219JF549
Janelia Fluor 646	NBP2-41219JF646
Janelia Fluor 669	NBP2-41219JF669
PE	NBP2-41219PE
PerCP	NBP2-41219PCP
mFluor Violet 450 SE	NBP2-41219MFV450
mFluor Violet 500 SE	NBP2-41219MFV500
mFluor Violet 610 SE	NBP2-41219MFV610

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NBP1-47433

B-Raf Antibody (1H12)

0.1 ml

NBP1-47433

Immunohistochemistry-Paraffin: B-Raf Antibody (1H12) [NBP1-47433] - Analysis of MCF-7 (left) and HepG2 (right) cells using BRAF mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-47555

B-Raf Antibody (1H12F1)

0.1 ml

NBP1-47555

Western Blot: B-Raf Antibody (1H12F1) [NBP1-47555] - Analysis using BRAF mouse mAb against truncated recombinant Braf (1) and A431 cell lysate (2).

Immunohistochemistry-Paraffin: B-Raf Antibody (1H12F1) [NBP1-47555] - Analysis of human testis tissues using BRAF mouse mAb.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

BRAF Products By Gene Name

B-Raf Antibody - BSA Free

B-Raf Antibody (OTI5A9)

B-Raf Antibody [Unconjugated]

B-Raf Antibody (RM8) - V600-E Mutation

B-Raf Antibody

B-Raf Antibody (IHC600) - V600-E Mutation

B-Raf Antibody (336016) [Unconjugated]

B-Raf Antibody (CL4004) - BSA Free

B-Raf Antibody (CL4003) - BSA Free

B-Raf Antibody - BSA Free

B-Raf Antibody (1H12)

B-Raf Antibody (1H12F1)