Axl Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1.0 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Axl as an approximately 140 kDa glycoprotein (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

A431 human epithelial carcinoma cell line was stained with Goat Anti-Human Axl Affinity-Purified Polyclonal Antibody (Catalog # AF154, filled histogram) or normal goat IgG control antibody (AB-108-C, open histogram) followed by APC-conjugated anti-Goat IgG Secondary Antibody (F0108). View our protocol for Staining Membrane-associated Proteins.

Axl was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Axl knockout A431 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Axl at approximately 150 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Axl knockout A431 human epithelial carcinoma cell line was stained with Goat Anti-Human Axl Affinity-Purified Polyclonal Antibody (Catalog # AF154, filled histogram) or normal goat IgG control antibody (AB-108C, open histogram) followed by APC-conjugated anti-Goat IgG Secondary Antibody (F0108). No staining was observed in the Axl knockout A431 cell line. View our protocol for Staining Membrane-associated Proteins.

Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for Axl at approximately 154 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

TGF beta promotes HLA-ABC downregulation at baseline and in response to IFN gamma .a IFN gamma -mediated induction (IFN gamma -treated/vehicle-treated control) of cell surface HLA-ABC in MITFhigh/AXLlow or /MITFlow/AXLhigh short-term PD-1 PROG melanoma cell lines. Each dot represents one cell line and HLA-ABC induction was measured by flow cytometry 24 h after treating cultures with vehicle control or 1000 U/ml IFN gamma . Box plots show the median and interquartile ranges, and data were compared using Mann-Whitney test. b Cell surface expression (median fluorescence intensity; MFI) of HLA-ABC in WMD-084, SCC14-0257 and SMU17-0132 melanoma cells treated with vehicle (Control), 1000 U/ml IFN gamma and/or 10 ng/ml TGF beta for 72 h. Data (mean ± s.d.) were compared using one-way ANOVA with the Geisser-Greenhouse correction. c Expression of de-differentiation markers AXL, N-cadherin and SNAIL in WMD-084, SCC14-0257 and SMU17-0132 melanoma cells treated with vehicle (Control), 1000 U/ml IFN gamma - and/or 10 ng/ml TGF beta for 72 h. d IFN gamma production after co-culture of TGF beta pre-treated (10 ng/ml for 72 h) melanoma cells with the patient-matched tumor-infiltrating lymphocytes expanded from the same tumor biopsy. IFN gamma was measured by flow cytometry. Data (mean ± s.d.) show relative IFN gamma expression in T cells (TGF beta pre-treated/BSA pre-treated) after background subtraction (spontaneous IFN gamma production on immune cell-only cultures). Paired BSA-treated vs TGF beta -treated data were compared using paired t-test, **p 25, n = 23 for CD133; n = 21 for Axl) or low (BMI ≤ 25, n = 13 for CD133; n = 11 for Axl) BMI PM/ER−/PR− patients’ tumor samples, stroma is marked in green, CD133− or Axl−/low cancer cells are marked in blue and positive staining cancer cells are marked in red (for c, P = 0.0245; d, P = 0.023). e Time-dependent proliferation assay of ex vivo E0771 cells isolated from chow or HFD-fed mice. For each time point, data are represented as mean ± SEM of four tumor samples (eight replicates per tumor sample were measured) from each group. f Tumorsphere formation assay of E0771 ex vivo cells isolated from chow diet or HFD-fed mice. Representative images of day-5 tumorspheres formation of E0771 ex vivo cells and red arrowheads mark the identified tumorspheres. Quantification of day-5 tumorspheres is represented as mean ± SEM of four tumor samples from each group and three replicates were measured for each sample, P = 0.0002. g Fatty acid and glucose oxidation on E0771 ex vivo cells isolated from two chow diet-fed mice and three HFD-fed mice. The oxidation data are normalized to cell protein content. For each sample, 8 replicates are measured and data are represented as mean ± SEM of all replicates from each group. Fatty acid oxidation P = 0.0002, Glucose oxidation P = 0.0365. For c, d, unpaired, two-tailed Welch’s t-test was used for statistical testing. For f, g, statistical significance determined with unpaired, two-tailed Student’s t-test (*P value <0.05; ***P value <0.001). Source data are provided as a Source data file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35013251), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of Axl and Gas6 in clinical samples. (A,B) Immunohistochemical analysis of Axl (A) or Gas6 (B) in non-small cell lung cancer tissues from patients who underwent surgery following preoperative chemotherapy or chemoradiotherapy. Insets show higher magnification of the areas indicated in the boxes. Scale bar, 50 μm. (A) Representative images showing tumor Axl-negative tumor tissues (left), and tumor Axl-positive tumor tissues (right). Tissues were stained with an anti-Axl antibody (brown) and counterstained with hematoxylin. (B) Representative images showing stromal Gas6-negative tumor tissues (left) and stromal Gas6-positive tumor tissues (right). Tissues were stained with an anti-Gas6 antibody (brown) and counterstained with hematoxylin. (C) Correlation between tumor Axl expression and stromal Gas6 expression in tumor tissues observed in (A and B). (D) Correlation between tumor Axl, stromal Gas6 expression and survival. Kaplan–Meier plot of disease-free survival in patients with non-small cell lung cancer who underwent surgery following preoperative chemotherapy or chemoradiotherapy, stratified according to tumor Axl and stromal Gas6 expression. Five-year disease-free survival in the patients with tumors expressing both tumor Axl and stromal Gas6 (n = 37) was significantly worse than in the both-negative group (n = 12) (21.9% vs 51.3%, p = 0.04). The five-year disease-free survival of tumor Axl-negative and stromal Gas6-positive group was 50.7%, and the difference in survival between this group and both-positive or both-negative group was not significant (p = 0.20 and 0.49, respectively); *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28878389), licensed under a CC-BY license. Not internally tested by R&D Systems.

rVSV∆G/EBOV entry kinetics: (A) VeroS cells were infected with rVSV∆G/EBOV at multiple MOIs, inoculum was removed after 1 h, and monitored for luciferase production over time. (B) VeroS, HEK293T, and HAP1 cells were infected with rVSV∆G/EBOV (MOI 1), inoculum was not removed, and monitored for luciferase production over time. Right panel zooms in to display the signals observed in HAP1 and HEK293T cells. (C) Immunoblots demonstrating production of the transfected receptors in HEK293T cells. (D) HEK293T cells transfected with the indicated receptors were infected with rVSV∆G/EBOV and monitored for luciferase production over time. Each experiment was repeated in duplicate, three independent times. Data shown are the averages of averages and corresponding SEM of three independent trials. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33348746), licensed under a CC-BY license. Not internally tested by R&D Systems.

Tyro3 increases Gas6-induced Axl phosphorylation.Rat2 (Rat lanes 1–3) and Rat2/T3V5 cells (Rat/T3, lanes 4–6) were treated with 0, 50, 350 ng/ml Gas6 for 20 min. Detergent cell lysates were prepared and normalized for protein concentration. The samples were divided in two for Tyro3 (panel A) and Axl (panel B) immunoprecipitations (IP). This was followed by SDS-PAGE using 8% gels followed and Western blot analysis. The membranes were probed with anti-phosphotyrosine ( alpha -pTyr) antibodies (PY20 and P99 mixture 1∶3,500) (top, panels A and B). The membranes were stripped and reprobed with alpha -Tyro3 serum 5424 (1∶3,500) (A, bottom panel) or affinity purified rabbit alpha -Axl (1∶3,500) (B, bottom panel). These blots are representative of 4 experiments. To determine whether Tyro3 expression affects Axl levels in Rat2 cells (panel C), detergent cell extracts were prepared from Rat2 cells (lane 1), and independently derived stably transfected Rat2/T3V5 cell lines (clone (cl) 25, lane 2; clone 11, lane 3; clone 30, lane 4). SDS-PAGE using 4–20% gels followed by Western blot analysis was performed on these extracts. The membranes were cut at the level of the 66 kDa marker and the top portion was probed with rabbit alpha -Tyro3 (5424 serum 1∶3,500) or rabbit alpha -Axl (1∶3,500). The bottom portion of the membranes were blotted with alpha -GAPDH (1∶500). These blots are representative of 5 experiments. To determine if the levels of Axl phosphorylation depended on the levels of Tyro3 expressed (panel D) Rat2 cells (lanes 1 and 2) and Rat2/T3V5 cell lines cl25 (lanes 3 and 4) and cl30 (lanes 5 and 6) were activated with media only (0) or 350 ng/ml of Gas6 for 10 min. Detergent cell lysates were prepared and normalized for protein concentration. The samples were divided in two for Tyro3 and Axl immunoprecipitations (IP). SDS-PAGE using 8% gels followed by Western blot analysis was performed. The membranes were probed with anti-phosphotyrosine ( alpha -pTyr) antibodies (PY20 and P99 mixture 1∶3,500) (top and third panels). An aliquot of the remaining Axl IPs were reloaded and probed with affinity purified rabbit alpha -Axl (1∶3,500) (second panel from the top). The membrane corresponding to Tyro3 IP’s was stripped and reprobed with alpha -Tyro3 serum 5424 (1∶3,500) (bottom panel). These blots are representative of 6 experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0036800), licensed under a CC-BY license. Not internally tested by R&D Systems.

Migration of H1299 NSCLC cells enhanced by ligand-dependent Axl activation. (A) Western blotting to assess Gas6 expression in H1299 cells. Expression of Gas6 in LCAFhTERT cells was used as a positive control. (B) Phosphorylation of Axl was analyzed by Western blotting of whole cell lysates using different antibodies. GAPDH was used as an internal control. H1299 cells were stimulated for 15 min with 400 nM recombinant human Gas6 (rGas6). H1299 cells were treated with or without TP-0903 (0.2 µmol/L) for 24 h. (C) Migration of H1299 cells was analyzed using rGas6 (400 nM) added to the lower chamber. H1299 cells were treated with or without TP-0903 (0.2 µmol/L) for 24 h. (D) Western blotting of conditioned medium from LCAFhTERT transfected with siGas6 or siScr (control) to assess whether they contains Gas6 secreted by CAFs. (E) Phosphorylation of Axl in H1299 cells analyzed by Western blotting after stimulation with conditioned medium from siRNA-transfected LCAFhTERT. H1299 cells were treated with or without TP-0903 (0.2 µmol/L) for 24 h. The medium (DMEM containing 10% FBS) was used as control. (F) Migration of H1299 cells analyzed using conditioned medium of siRNA-transfected LCAFhTERT. H1299 cells were treated with or without TP-0903 (0.2 µmol/L) for 24 h. The medium (DMEM containing 10% FBS) was used as control. The relative number of migrated H1299 cells is indicated on the y-axis. Data show the mean ± SEM (n = 3); **p < 0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28878389), licensed under a CC-BY license. Not internally tested by R&D Systems.

Migration of H1299 NSCLC cells enhanced by ligand-dependent Axl activation. (A) Western blotting to assess Gas6 expression in H1299 cells. Expression of Gas6 in LCAFhTERT cells was used as a positive control. (B) Phosphorylation of Axl was analyzed by Western blotting of whole cell lysates using different antibodies. GAPDH was used as an internal control. H1299 cells were stimulated for 15 min with 400 nM recombinant human Gas6 (rGas6). H1299 cells were treated with or without TP-0903 (0.2 µmol/L) for 24 h. (C) Migration of H1299 cells was analyzed using rGas6 (400 nM) added to the lower chamber. H1299 cells were treated with or without TP-0903 (0.2 µmol/L) for 24 h. (D) Western blotting of conditioned medium from LCAFhTERT transfected with siGas6 or siScr (control) to assess whether they contains Gas6 secreted by CAFs. (E) Phosphorylation of Axl in H1299 cells analyzed by Western blotting after stimulation with conditioned medium from siRNA-transfected LCAFhTERT. H1299 cells were treated with or without TP-0903 (0.2 µmol/L) for 24 h. The medium (DMEM containing 10% FBS) was used as control. (F) Migration of H1299 cells analyzed using conditioned medium of siRNA-transfected LCAFhTERT. H1299 cells were treated with or without TP-0903 (0.2 µmol/L) for 24 h. The medium (DMEM containing 10% FBS) was used as control. The relative number of migrated H1299 cells is indicated on the y-axis. Data show the mean ± SEM (n = 3); **p < 0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28878389), licensed under a CC-BY license. Not internally tested by R&D Systems.

HER2/HER3 and AXL expression and phosphorylation analysis.A) Representative western blots of total cell lysates of HCC827 and HCC4006 parental cell lines (P) and their derived ERL-resistant cell lines. Arrows indicate the expected molecular weight size. Total cell lysates loaded were 40 μg for AXL and pAXL analyses and 25 μg for the others. B) qPCR analysis of AXL mRNA normalized to rp-L31 mRNA and expressed relative to the levels in parental cell lines set as 1 (mean ± SD of triplicate determinations). Western blots and qPCR data are representative of those obtained respectively from 3 and 2 independent analysis. C) Dose-effect curves were calculated using CompuSyn software and plotting the entered Fa values against the entered dose values. For combination treatments, the combined drugs dose was entered. Each data point represents the mean of 3 replicates. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0143333), licensed under a CC-BY license. Not internally tested by R&D Systems.

The expression of p-AXL&AXL positively correlates with the expression of KLF5 in human TNBC specimens. Moreover, DCC-2036 increases the sensitivity of TNBC chemotherapy by decreasing BCSCs.A Representative immunohistochemical staining images of p-AXL, AXL,&KLF5 protein in TNBC specimens, in which the expression of p-AXL, AXL,&KLF5 proteins was indicated by mild positive (+), moderate positive (++),&strong positive (+++), respectively. Left: Scale bars, 200 μm (magnification 40×); Right: Scale bars, 50 μm (magnification 100×). Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36042208), licensed under a CC-BY license. Not internally tested by R&D Systems.

DCC-2036 inhibits CSCs in vivo according to the limited dilution assay by pretreating 4T1 cells with DCC-2036 or DMSO for 48 h.A In vivo tumorigenicity assay with limited dilution using DMSO or DCC-2036 treated 4T1 cells respectively: 200,000 (n = 7 and 8), 20,000 (n = 9 and 8), or 2000 (n = 10 and 10) cells per injection site. The frequency of BCSCs was calculated by ELDA. B Weight variation of mice following transplant of 4T1 cells into BALB/C mice after 7 days. C Images of tumor formation in BALB/C mice. D, E Transplanted tumors were harvested, and the tumor size and weight were measured at the end of the experiment. The statistical significance was determined by Student’s t-test. F Tumor-free survival curve of BALB/C mice is shown. G Hematoxylin and eosin (H&E) staining indicates the histology of tumor tissues. Scale bars, 20 μm. H Immunohistochemical analysis using p-AXL, AXL, and KLF5 antibodies in xenograft tissues from BALB/C mice. Scale bars, 20 μm. I Immunoblot of transplanted tumors from BALB/C mice posterior to the experiments. C means Control, T means DCC-2036. We chose the tumor tissues randomly for H&E staining, immunohistochemical analysis, and immunoblotting. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36042208), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western, Flow, IHC, B/N, CyTOF-ready, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Axl Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Axl
  Glu33-Pro440
  Accession # AAA61243
• Specificity: Detects human Axl in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse Axl is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AXL
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Axl Antibody [Unconjugated]

• AI323647
• ARK
• AXL oncogene
• AXL receptor tyrosine kinase
• AXL transforming sequence/gene
• Axl
• EC 2.7.10
• EC 2.7.10.1
• JTK11
• Tyro7
• tyrosine-protein kinase receptor UFO
• UFO
• UFOoncogene AXL

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
3. Holland, S. et al. (2005) Canc. Res. 65:9294.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Axl Antibody (AF154)(121)

Publications using AF154

• Messingham, KN;Richards, PT;Fleck, A;Patel, RA;Djurkovic, M;Elliff, J;Connell, S;Crowe, TP;Munoz Gonzalez, J;Gourronc, F;Dillard, JA;Davey, RA;Klingelhutz, A;Shtanko, O;Maury, W; Multiple cell types support productive infection and dynamic translocation of infectious Ebola virus to the surface of human skin Science advances 2025-01-03 [PMID: 39742475] (Flow Cytometry, Human)
• Kendsersky, NM;Odrobina, M;Mabe, NW;Farrel, A;Grossmann, L;Tsang, M;Groff, D;Wolpaw, AJ;Zammarchi, F;van Berkel, PH;Dang, CV;Mossé, YP;Stegmaier, K;Maris, JM; Lineage-dependence of the neuroblastoma surfaceome defines tumor cell state-dependent and independent immunotherapeutic targets bioRxiv : the preprint server for biology 2024-07-02 [PMID: 39005383] (Western Blot, Human)
• Raymond, D;Fukui, M;Zwernik, S;Kassam, A;Rovin, R;Akhtar, P; Evaluating soluble Axl as a biomarker for glioblastoma: A pilot study PloS one 2024-07-05 [PMID: 38968207] (Western Blot, Human)
• Warner van Dijk, FA;Tong, O;O'Neil, TR;Bertram, KM;Hu, K;Baharlou, H;Vine, EE;Jenns, K;Gosselink, MP;Toh, JW;Papadopoulos, T;Barnouti, L;Jenkins, GJ;Sandercoe, G;Haniffa, M;Sandgren, KJ;Harman, AN;Cunningham, AL;Nasr, N; Characterising plasmacytoid and myeloid AXL+ SIGLEC-6+ dendritic cell functions and their interactions with HIV PLoS pathogens 2024-06-26 [PMID: 38924030] (Immunohistochemistry, Human)
• Justynski, O;Bridges, K;Krause, W;Forni, MF;Phan, QM;Sandoval-Schaefer, T;Carter, K;King, DE;Hsia, HC;Gazes, MI;Vyce, SD;Driskell, RR;Miller-Jensen, K;Horsley, V; Apoptosis recognition receptors regulate skin tissue repair in mice eLife 2023-12-21 [PMID: 38127424] (In Vivo, Mouse)
• Creixell, M;Taylor, SD;Gerritsen, J;Bae, SY;Jiang, M;Augustin, T;Loui, M;Boixo, C;Creixell, P;White, FM;Meyer, AS; Dissecting signaling regulators driving AXL-mediated bypass resistance and associated phenotypes by phosphosite perturbations bioRxiv : the preprint server for biology 2023-10-23 [PMID: 37961516] (Bioassay, Human)
• Kristina Breitenecker, Viola Hedrich, Franziska Pupp, Doris Chen, Eva Řezníčková, Gregor Ortmayr et al. Synergism of the receptor tyrosine kinase Axl with ErbB receptors mediates resistance to regorafenib in hepatocellular carcinoma Front Oncol 2023-09-08 [PMID: 37746265]
• Sakemura, RL;Hefazi, M;Cox, MJ;Siegler, EL;Sinha, S;Hansen, MJ;Stewart, CM;Feigin, JM;Manriquez Roman, C;Schick, KJ;Can, I;Tapper, EE;Horvei, P;Adada, MM;Bezerra, ED;Kankeu Fonkoua, LA;Ruff, MW;Forsman, CL;Nevala, WK;Boysen, JC;Tschumper, RC;Grand, CL;Kuchimanchi, KR;Mouritsen, L;Foulks, JM;Warner, SL;Call, TG;Parikh, SA;Ding, W;Kay, NE;Kenderian, SS; AXL inhibition improves the anti-tumor activity of chimeric antigen receptor T cells Cancer immunology research 2023-06-28 [PMID: 37378662] (Flow Cytometry, Human)
• Serwe, G;Kachaner, D;Gagnon, J;Plutoni, C;Lajoie, D;Duramé, E;Sahmi, M;Garrido, D;Lefrançois, M;Arseneault, G;Saba-El-Leil, MK;Meloche, S;Emery, G;Therrien, M; CNK2 promotes cancer cell motility by mediating ARF6 activation downstream of AXL signalling Nature communications 2023-06-15 [PMID: 37322019] (Neutralization, Human)
• Hedrich, V;Breitenecker, K;Ortmayr, G;Pupp, F;Huber, H;Chen, D;Sahoo, S;Jolly, MK;Mikulits, W; PRAME Is a Novel Target of Tumor-Intrinsic Gas6/Axl Activation and Promotes Cancer Cell Invasion in Hepatocellular Carcinoma Cancers 2023-04-22 [PMID: 37173882] (Bioassay, Human)
• Oltin-Tiberiu Pop, Anne Geng, Emilio Flint, Arjuna Singanayagam, Caner Ercan, Lucia Possamai et al. AXL Expression on Homeostatic Resident Liver Macrophages Is Reduced in Cirrhosis Following GAS6 Production by Hepatic Stellate Cells Cellular and Molecular Gastroenterology and Hepatology 2023-03-31 [PMID: 37004869]
• SY Lim, E Shklovskay, JH Lee, B Pedersen, A Stewart, Z Ming, M Irvine, B Shivalinga, RPM Saw, AM Menzies, MS Carlino, RA Scolyer, GV Long, H Rizos The molecular and functional landscape of resistance to immune checkpoint blockade in melanoma Nature Communications, 2023-03-18;14(1):1516. 3/18/2023 [PMID: 36934113]
• K Ning, W Zou, P Xu, F Cheng, EY Zhang, A Zhang-Chen, S Kleiboeker, J Qiu Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors Science Advances, 2023-01-11;9(2):eade0869. 2023-01-11 [PMID: 36630517] (ICC, Neutralization, Human)
• F Zito Marin, CM Della Cort, V Ciaramella, S Erra, A Ronchi, A Fiorelli, G Vicidomini, M Santini, G Scognamigl, F Morgillo, F Ciardiello, R Franco, M Accardo AXL and MET Tyrosine Kinase Receptors Co-Expression as a Potential Therapeutic Target in Malignant Pleural Mesothelioma Journal of personalized medicine, 2022-12-02;12(12):. 2022-12-02 [PMID: 36556214] (ICC, IHC, Human)
• Jeff Hsu, Colin Chong, Jeffrey Serrill, Levina Goon, Joan Balayan, Eric N. Johnson et al. Preclinical Characterization of XL092, a Novel Receptor Tyrosine Kinase Inhibitor of MET, VEGFR2, AXL, and MER Molecular Cancer Therapeutics 2023-02-01 [PMID: 36399631]
• A Alli-Shaik, B Qiu, SL Lai, N Cheung, G Tan, SP Neo, A Tan, CMG Cheung, W Hong, TY Wong, X Wang, J Gunaratne System-wide vitreous proteome dissection reveals impaired sheddase activity in diabetic retinopathy Theranostics, 2022-09-11;12(15):6682-6704. 2022-09-11 [PMID: 36185601] (Western Blot, Human)
• M New-Aaron, RS Dagur, SS Koganti, M Ganesan, W Wang, E Makarov, M Ogunnaike, KK Kharbanda, LY Poluektova, NA Osna Alcohol and HIV-Derived Hepatocyte Apoptotic Bodies Induce Hepatic Stellate Cell Activation Biology, 2022-07-14;11(7):. 7/14/2022 [PMID: 36101437]
• Y Shen, Q Zhu, M Xiao, L Yin, W Feng, J Feng, J He, P Li, X Chen, W Ding, J Zhong, Z Zeng, Z Xie, J Liu, X Zu Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop Cell Death & Disease, 2022-08-30;13(8):749. 2022-08-30 [PMID: 36042208] (IHC, Human)
• H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein Nature Medicine, 2022-08-04;0(0):. 2022-08-04 [PMID: 35927581] (Western Blot, Human)
• N Comandante, DG Baumann, M Fallahi-Si AP-1 transcription factor network explains diverse patterns of cellular plasticity in melanoma cells Cell Reports, 2022-08-02;40(5):111147. 2022-08-02 [PMID: 35926467] (ICC/IF, Human)
• Agata Poświata, Kamila Kozik, Marta Miączyńska, Daria Zdżalik-Bielecka Endocytic trafficking of GAS6–AXL complexes is associated with sustained AKT activation Cellular and Molecular Life Sciences 2022-06-01 [PMID: 35622156] (Immunocytochemistry, Human)
• N Al Kafri, J Ahnström, A Teraz-Oros, L Chaput, N Singh, BO Villoutrei, S Hafizi The first laminin G-like domain of protein S is essential for binding and activation of Tyro3 receptor and intracellular signalling Biochemistry and Biophysics Reports, 2022-04-28;30(0):101263. 2022-04-28 [PMID: 35518197] (Western Blot, Human)
• M Nyakas, KG Fleten, MH Haugen, N Engedal, C Sveen, IN Farstad, VA Flørenes, L Prasmickai, GM Mælandsmo, K Seip AXL inhibition improves BRAF-targeted treatment in melanoma Scientific Reports, 2022-03-24;12(1):5076. 2022-03-24 [PMID: 35332208] (IHC, Human)
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• Samuel D. Zwernik, Beau H. Adams, Daniel A. Raymond, Catherine M. Warner, Amin B. Kassam, Richard A. Rovin et al. AXL receptor is required for Zika virus strain MR-766 infection in human glioblastoma cell lines Molecular Therapy - Oncolytics 2021-12-17 [PMID: 34901388] (Western Blot, Neutralization, Human)
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• MJ Gattas, IG Estecho, MA Lago Huvel, AE Errasti, EA Carrera Si, M Simian A Heterotypic Tridimensional Model to Study the Interaction of Macrophages and Glioblastoma In Vitro International Journal of Molecular Sciences, 2021-05-12;22(10):. 2021-05-12 [PMID: 34065977] (Flow Cytometry, Human)
• F Wanke, S Gutbier, A Rümmelin, M Steinberg, LD Hughes, M Koenen, J Komuczki, D Regan-Komi, S Wagage, J Hesselmann, R Thoma, D Brugger, T Christopei, H Wang, F Point, R Hallet, S Ghosh, CV Rothlin, C Patsch, B Geering Ligand-dependent kinase activity of MERTK drives efferocytosis in human iPSC-derived macrophages Cell Death & Disease, 2021-05-25;12(6):538. 2021-05-25 [PMID: 34035216] (Flow Cytometry, Human)
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• Benjamin L. Emert, Christopher J. Cote, Eduardo A. Torre, Ian P. Dardani, Connie L. Jiang, Naveen Jain et al. Variability within rare cell states enables multiple paths towards drug resistance Nature Biotechnology 2021-07-01 [PMID: 33619394]
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• K Kongmanas, N Punyadee, K Wasuworawo, A Songjaeng, T Prommool, Y Pewkliang, S Manocheewa, S Thiemmeca, K Sa-Ngiamsu, C Puttikhunt, KF Faull, S Hongeng, P Avirutnan Immortalized stem cell-derived hepatocyte-like cells: An alternative model for studying dengue pathogenesis and therapy PLoS Negl Trop Dis, 2020-11-20;14(11):e0008835. 2020-11-20 [PMID: 33216752] (Flow Cytometry, Human)
• F Rossi, B Josey, EC Sayitoglu, R Potens, T Sultu, AD Duru, V Beljanski Characterization of zika virus infection of human fetal cardiac mesenchymal stromal cells PLoS ONE, 2020-09-17;15(9):e0239238. 2020-09-17 [PMID: 32941515] (Neutralization, Human)
• R Wang, T Yamada, K Kita, H Taniguchi, S Arai, K Fukuda, M Terashima, A Ishimura, A Nishiyama, A Tanimoto, S Takeuchi, K Ohtsubo, K Yamashita, T Yamano, A Yoshimura, K Takayama, K Kaira, Y Taniguchi, S Atagi, H Uehara, R Hanayama, I Matsumoto, X Han, K Matsumoto, W Wang, T Suzuki, S Yano Transient IGF-1R inhibition combined with osimertinib eradicates AXL-low expressing EGFR mutated lung cancer Nat Commun, 2020-09-14;11(1):4607. 2020-09-14 [PMID: 32929081] (IHC, Human)
• J Hong, F Abid, S Phillips, SN Salaria, FL Revetta, D Peng, MK Washington, W El-Rifai, A Belkhiri Co-overexpression of AXL and c-ABL predicts a poor prognosis in esophageal adenocarcinoma and promotes cancer cell survival Journal of Cancer, 2020-08-08;11(20):5867-5879. 8/8/2020 [PMID: 32922529]
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• K Flem-Karls, M Nyakas, IN Farstad, E McFadden, P Wernhoff, KD Jacobsen, VA Flørenes, GM Mælandsmo Soluble AXL as a marker of disease progression and survival in melanoma PLoS ONE, 2020-01-09;15(1):e0227187. 1/9/2020 [PMID: 31917795]
• Greiffo FR, Viteri-Alvarez V, Frankenberger M et al. CX3CR1-fractalkine axis drives kinetic changes of monocytes in fibrotic interstitial lung diseases Eur. Respir. J. 11/19/2019 [PMID: 31744836]
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• LA Koopman, MG Terp, GG Zom, ML Janmaat, K Jacobsen, E Gresnigt-V, M Brandhorst, U Forssmann, FM de Bree, N Pencheva, A Lingnau, MA Zipeto, PW Parren, EC Breij, HJ Ditzel Enapotamab vedotin, an AXL-specific antibody-drug conjugate, shows preclinical antitumor activity in non-small cell lung cancer JCI Insight, 2019-11-01;0(0):. 2019-11-01 [PMID: 31600169] (Western Blot, Human)
• Y Xiao, H Zhao, L Tian, R Nolley, AN Diep, A Ernst, KC Fuh, YR Miao, R von Eyben, JT Leppert, JD Brooks, DM Peehl, AJ Giaccia, EB Rankin S100A10 is a critical mediator of GAS6/AXL-induced angiogenesis in renal cell carcinoma Cancer Res., 2019-10-04;0(0):. 2019-10-04 [PMID: 31585940] (IHC, Human)
• A Carestia, HA Mena, CM Olexen, JM Ortiz Wilc, S Negrotto, AE Errasti, RM Gómez, CN Jenne, EA Carrera Si, M Schattner Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice Cell Rep, 2019-07-23;28(4):896-908.e5. 2019-07-23 [PMID: 31340152] (Neutralization, Human)
• DP Strange, B Jiyarom, N Pourhabibi, X Xie, C Baker, H Sadri-Arde, PY Shi, S Verma Axl Promotes Zika Virus Entry and Modulates the Antiviral State of Human Sertoli Cells MBio, 2019-07-16;10(4):. 2019-07-16 [PMID: 31311882] (Neutralization, Human)
• Y Duan, B Hu, C Qiao, L Luo, X Li, J Wang, H Liu, T Zhou, B Shen, M Lv, J Feng Engineered AXL(-ECD)-Fc variants that abolish the AXL/Gas6 interaction suppress tumor cell migration. Oncol Lett, 2019-04-15;17(6):5784-5792. 2019-04-15 [PMID: 31186805] (Flow Cytometry, Human)
• KA Mills, JM Quinn, ST Roach, M Palisoul, M Nguyen, H Noia, L Guo, J Fazal, DG Mutch, SA Wickline, H Pan, KC Fuh p5RHH nanoparticle-mediated delivery of AXL siRNA inhibits metastasis of ovarian and uterine cancer cells in mouse xenografts Sci Rep, 2019-03-18;9(1):4762. 2019-03-18 [PMID: 30886159] (IHC-P, Western Blot, Human, Xenograft)
• L Sinik, KA Minson, JJ Tentler, J Carrico, SM Bagby, WA Robinson, R Kami, T Burstyn-Co, SG Eckhardt, X Wang, SV Frye, HS Earp, D DeRyckere, DK Graham Inhibition of MERTK Promotes Suppression of Tumor Growth in BRAF Mutant and BRAF Wild-Type Melanoma Mol. Cancer Ther., 2018-11-27;18(2):278-288. 2018-11-27 [PMID: 30482852] (Western Blot, Human)
• Nellie K. McDaniel, Christopher T. Cummings, Mari Iida, Justus Hülse, Hannah E. Pearson, Eleana Vasileiadi et al. MERTK Mediates Intrinsic and Adaptive Resistance to AXL-targeting Agents Molecular Cancer Therapeutics 2018-11-01 [PMID: 30093568] (Immunoprecipitation, Human)
• Carl A Ascoli, Birte Aggeler Overlooked benefits of using polyclonal antibodies BioTechniques 2018-09-01 [PMID: 30089399] (Western Blot, Human)
• Lin J. R, Izar B, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7/11/2018 [PMID: 29993362]
  
  Details:
  Citation using the Alexa Fluor 488 format of this antibody.
• Heidi Jaksch-Bogensperger, Anna Hammerschmid, Ludwig Aigner, Eugen Trinka, Renate Gehwolf, Yvonne Ebner et al. Proseek single-plex protein assay kit system to detect sAxl and Gas6 in serological material of brain tumor patients Biotechnology Reports 2018-06-01 [PMID: 29876303]
• Rachel B. Brouillette, Elisabeth K. Phillips, Radhika Patel, Wadie Mahauad-Fernandez, Sven Moller-Tank, Kai J. Rogers et al. TIM-1 Mediates Dystroglycan-Independent Entry of Lassa Virus Journal of Virology 2018-08-15 [PMID: 29875238]
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• Q Guo, T Grimmig, G Gonzalez, A Giobbie-Hu, G Berg, N Carr, BJ Wilson, P Banerjee, J Ma, JS Gold, B Nandi, Q Huang, AM Waaga-Gass, CG Lian, GF Murphy, MH Frank, M Gasser, NY Frank ATP-binding cassette member B5 (ABCB5) promotes tumor cell invasiveness in human colorectal cancer J. Biol. Chem., 2018-05-22;0(0):. 2018-05-22 [PMID: 29789423] (Flow Cytometry, Western Blot, Human, Xenograft)
• A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman Human Sertoli cells support high levels of Zika virus replication and persistence Sci Rep, 2018-04-03;8(1):5477. 2018-04-03 [PMID: 29615760] (Neutralization, Human)
• M Persaud, A Martinez-L, C Buffone, SA Porcelli, F Diaz-Griff Infection by Zika viruses requires the transmembrane protein AXL, endocytosis and low pH Virology, 2018-03-22;518(0):301-312. 2018-03-22 [PMID: 29574335] (Flow Cytometry, Human)
• Irene Scharf, Lisa Bierbaumer, Heidemarie Huber, Philipp Wittmann, Christine Haider, Christine Pirker et al. Dynamics of CRISPR/Cas9-mediated genomic editing of the AXL locus in hepatocellular carcinoma cells Oncology Letters 2017-12-13 [PMID: 29434956]
• Melissa J. Mulla, Ingrid C. Weel, Julie A. Potter, Stefan M. Gysler, Jane E. Salmon, Maria T. S. Peraçoli et al. Antiphospholipid Antibodies Inhibit Trophoblast Toll-Like Receptor and Inflammasome Negative Regulators Arthritis & Rheumatology 2018-06-01 [PMID: 29342502]
• BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono Protein S and Gas6 induce efferocytosis of HIV-1-infected cells Virology, 2018-01-02;515(0):176-190. 2018-01-02 [PMID: 29304470] (Neutralization, Human)
• NB Leighl, MS Tsao, G Liu, D Tu, C Ho, FA Shepherd, N Murray, JR Goffin, G Nicholas, S Sakashita, Z Chen, L Kim, J Powers, L Seymour, G Goss, PA Bradbury A phase I study of foretinib plus erlotinib in patients with previously treated advanced non-small cell lung cancer: Canadian cancer trials group IND.196 Oncotarget, 2017-06-28;8(41):69651-69662. 2017-06-28 [PMID: 29050231] (IHC, Human)
• C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma Br. J. Cancer, 2017-10-12;117(12):1787-1797. 2017-10-12 [PMID: 29024938] (Flow Cytometry, Human)
• Luís Eduardo Zucca, Mariana Andozia Morini Matushita, Renato José da Silva Oliveira, Cristovam Scapulatempo-Neto, Marcos Alves de Lima, Guilherme Gomes Ribeiro et al. Expression of tyrosine kinase receptor AXL is associated with worse outcome of metastatic renal cell carcinomas treated with sunitinib Urologic Oncology: Seminars and Original Investigations 2018-01-01 [PMID: 28986088]
• SN Cross, JA Potter, P Aldo, JY Kwon, M Pitruzzell, M Tong, S Guller, CV Rothlin, G Mor, VM Abrahams Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation J. Immunol., 2017-09-15;0(0):. 2017-09-15 [PMID: 28916522] (Neutralization, Western Blot, Human)
• R Kanzaki, H Naito, K Kise, K Takara, D Eino, M Minami, Y Shintani, S Funaki, T Kawamura, T Kimura, M Okumura, N Takakura Gas6 derived from cancer-associated fibroblasts promotes migration of Axl-expressing lung cancer cells during chemotherapy Sci Rep, 2017-09-06;7(1):10613. 2017-09-06 [PMID: 28878389] (Western Blot, Human)
• M Seike, CH Kim, F Zou, R Noro, M Chiba, A Ishikawa, S ?unugi, K Kubota, A Gemma AXL and GAS6 co-expression in lung adenocarcinoma as a prognostic classifier Oncol. Rep., 2017-04-21;0(0):. 4/21/2017 [PMID: 28440492]
• AK Hastings, LJ Yockey, BW Jagger, J Hwang, R Uraki, HF Gaitsch, LA Parnell, B Cao, IU Mysorekar, CV Rothlin, E Fikrig, MS Diamond, A Iwasaki TAM Receptors Are Not Required for Zika Virus Infection in Mice Cell Rep, 2017-04-18;19(3):558-568. 2017-04-18 [PMID: 28423319] (Western Blot, Human, Mouse)
• AS Richard, BS Shim, YC Kwon, R Zhang, Y Otsuka, K Schmitt, F Berri, MS Diamond, H Choe AXL-dependent infection of human fetal endothelial cells distinguishes Zika virus from other pathogenic flaviviruses Proc. Natl. Acad. Sci. U.S.A, 2017-02-06;114(8):2024-2029. 2017-02-06 [PMID: 28167751] (Neutralization, Human, Primate)
• KM Aagaard, A Lahon, MA Suter, RP Arya, MD Seferovic, MB Vogt, M Hu, F Stossi, MA Mancini, RA Harris, M Kahr, C Eppes, M Rac, MA Belfort, CS Park, D Lacorazza, R Rico-Hesse Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication Sci Rep, 2017-01-27;7(0):41389. 2017-01-27 [PMID: 28128342] (ICC, Human)
• Implication of the Receptor Tyrosine Kinase AXL in Head and Neck Cancer Progression Int J Mol Sci, 2016-12-22;18(1):. 2016-12-22 [PMID: 28025482] (IHC, Human)
• Zhi-Min Yuan AXL receptor signalling suppresses p53 in melanoma through stabilization of the MDMX-MDM2 complex J Mol Cell Biol, 2017-04-01;0(0):. 2017-04-01 [PMID: 27927748] (Western Blot, Human)
• Zika virus cell tropism in the developing human brain and inhibition by azithromycin Proc. Natl. Acad. Sci. U.S.A., 2016-11-29;113(50):14408-14413. 2016-11-29 [PMID: 27911847] (Neutralization, Human, Primate)
• Yi-Ran Cai, Yu-Jie Dong, Hong-Bo Wu, Da-Ping Yu, Li-Juan Zhou, Dan Su et al. Expression level of CRKL and AXL combined with exon 19 deletion in EGFR and ALK status confer differential prognosis of lung adenocarcinoma subtypes Oncology Letters 2016-11-01 [PMID: 27899998]
• Mihalis S Kariolis Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies J. Clin. Invest, 2016-11-28;0(0):. 2016-11-28 [PMID: 27893463] (Western Blot, Mouse)
• Joon-Suk Park, ChuHee Lee, Hyun-Kyoung Kim, Dayea Kim, Jung Beom Son, Eunhwa Ko et al. Suppression of the metastatic spread of breast cancer by DN10764 (AZD7762)-mediated inhibition of AXL signaling Oncotarget 2016-12-13 [PMID: 27829217]
• LM Divine, MR Nguyen, E Meller, RA Desai, B Arif, EB Rankin, KH Bligard, C Meyerson, IS Hagemann, M Massad, PH Thaker, AR Hagemann, CK McCourt, MA Powell, DG Mutch, KC Fuh AXL modulates extracellular matrix protein expression and is essential for invasion and metastasis in endometrial cancer Oncotarget, 2016-11-22;7(47):77291-77305. 2016-11-22 [PMID: 27764792] (IHC, Human)
• ZEB1-mediated melanoma cell plasticity enhances resistance to MAPK inhibitors EMBO Mol Med, 2016-10-04;0(0):. 2016-10-04 [PMID: 27596438] (Western Blot, Human)
• Cotargeting MNK and MEK kinases induces the regression of NF1-mutant cancers J Clin Invest, 2016-05-09;0(0):. 2016-05-09 [PMID: 27159396] (Western Blot, Human)
• K Sato, K Suda, S Shimizu, K Sakai, H Mizuuchi, K Tomizawa, T Takemoto, K Nishio, T Mitsudomi Clinical, Pathological, and Molecular Features of Lung Adenocarcinomas with AXL Expression PLoS ONE, 2016-04-21;11(4):e0154186. 2016-04-21 [PMID: 27100677] (IHC-P, Human)
• KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles J. Biol. Chem., 2016-03-22;291(20):10586-601. 2016-03-22 [PMID: 27006397] (Flow Cytometry, Western Blot, Human)
• Nalwoga H, Ahmed L, Arnes J, Wabinga H, Akslen L Strong Expression of Hypoxia-Inducible Factor-1alpha (HIF-1alpha) Is Associated with Axl Expression and Features of Aggressive Tumors in African Breast Cancer. PLoS ONE, 2016-01-13;11(1):e0146823. 1/13/2016 [PMID: 26760782]
• D N Debruyne, N Bhatnagar, B Sharma, W Luther, N F Moore, N-K Cheung et al. ALK inhibitor resistance in ALK(F1174L)-driven neuroblastoma is associated with AXL activation and induction of EMT Oncogene 2016-07-01 [PMID: 26616860]
• Presutti D, Santini S, Cardinali B et al. MET Gene Amplification and MET Receptor Activation Are Not Sufficient to Predict Efficacy of Combined MET and EGFR Inhibitors in EGFR TKI-Resistant NSCLC Cells. PLoS ONE. 11/20/2015 [PMID: 26580964]
• Lijun Zhou, Xian-De Liu, Mianen Sun, Xuesong Zhang, Peter German, Shanshan Bai et al. Targeting MET and AXL overcomes resistance to sunitinib therapy in renal cell carcinoma Oncogene 2016-05-01 [PMID: 26364599]
• Fritz H, Gustafsson A, Ljungberg B, Ceder Y, Axelson H, Dahlback B The Axl-Regulating Tumor Suppressor miR-34a Is Increased in ccRCC but Does Not Correlate with Axl mRNA or Axl Protein Levels. PLoS ONE, 2015-08-19;10(8):e0135991. 2015-08-19 [PMID: 26287733] (Flow Cytometry, Human)
• Christopher T. Cummings, Weihe Zhang, Kurtis D. Davies, Gregory D. Kirkpatrick, Dehui Zhang, Deborah DeRyckere et al. Small Molecule Inhibition of MERTK Is Efficacious in Non–Small Cell Lung Cancer Models Independent of Driver Oncogene Status Molecular Cancer Therapeutics 2015-09-01 [PMID: 26162689]
• Alisa B. Lee-Sherick, Weihe Zhang, Kelly K. Menachof, Amanda A. Hill, Sean Rinella, Gregory Kirkpatrick et al. Efficacy of a Mer and Flt3 tyrosine kinase small molecule inhibitor, UNC1666, in acute myeloid leukemia Oncotarget 2015-03-30 [PMID: 25762638]
• Christopher T. Cummings, Rachel M.A. Linger, Rebecca A. Cohen, Susan Sather, Gregory D. Kirkpatrick, Kurtis D. Davies et al. Mer590, a novel monoclonal antibody targeting MER receptor tyrosine kinase, decreases colony formation and increases chemosensitivity in non-small cell lung cancer Oncotarget 2014-06-26 [PMID: 25372020]
• Mihalis S. Kariolis, Yu Rebecca Miao, Douglas S. Jones, Shiven Kapur, Irimpan I. Mathews, Amato J. Giaccia et al. An engineered Axl 'decoy receptor' effectively silences the Gas6-Axl signaling axis Nature Chemical Biology 2014-11-01 [PMID: 25242553]
• DA-PING YU, YU-JIE DONG, HAI-QING ZHANG, JING-HUI WANG, YANG QU, LI-JUAN ZHOU et al. Differential expression of CRKL and AXL genes in lung adenocarcinoma subtypes according to the epidermal growth factor receptor and anaplastic lymphoma kinase gene status Biomedical Reports 2014-07-01 [PMID: 24944792]
• M. Batlle, P. Recarte-Pelz, E. Roig, M.A. Castel, M. Cardona, M. Farrero et al. AXL receptor tyrosine kinase is increased in patients with heart failure International Journal of Cardiology 2014-05-15 [PMID: 24681018]
• Kristina H. Knubel, Ben M. Pernu, Alexandra Sufit, Sarah Nelson, Angela M. Pierce, Amy K. Keating MerTK inhibition is a novel therapeutic approach for glioblastoma multiforme Oncotarget 2014-03-15 [PMID: 24658326]
• Ji , Wonjun, Choi , Chang-Mi, Rho , Jin Kyun, Jang , Se Jin, Park , Young So, Chun , Sung-Min, Kim , Woo Sung, Lee , Jung-Shi, Kim , Sang-We, Lee , Dae Ho, Lee , Jae Cheo Mechanisms of acquired resistance to EGFR-tyrosine kinase inhibitor in Korean patients with lung cancer. BMC Cancer, 2013-12-27;13(0):606. 2013-12-27 [PMID: 24369725] (IHC-P, Human)
• Rho , Jin Kyun, Choi , Yun Jung, Kim , Seon Ye, Kim , Tae Won, Choi , Eun Kyun, Yoon , Seon-Joo, Park , Bu Man, Park , Eunhye, Bae , Jong Hwa, Choi , Chang-Mi, Lee , Jae Cheo MET and AXL inhibitor NPS-1034 exerts efficacy against lung cancer cells resistant to EGFR kinase inhibitors because of MET or AXL activation. Cancer Res, 2013-10-28;74(1):253-62. 2013-10-28 [PMID: 24165158] (Western Blot, Human)
• Sandra Christoph, Deborah DeRyckere, Jennifer Schlegel, J. Kimble Frazer, Lance A. Batchelor, Alesia Y. Trakhimets et al. UNC569, a Novel Small-Molecule Mer Inhibitor with Efficacy against Acute Lymphoblastic Leukemia In Vitro and In Vivo Molecular Cancer Therapeutics 2013-11-01 [PMID: 23997116]
• Aaron S. Meyer, Miles A. Miller, Frank B. Gertler, Douglas A. Lauffenburger The Receptor AXL Diversifies EGFR Signaling and Limits the Response to EGFR-Targeted Inhibitors in Triple-Negative Breast Cancer Cells Science Signaling 2013-08-06 [PMID: 23921085]
• Jun Hong, DunFa Peng, Zheng Chen, Vikas Sehdev, Abbes Belkhiri ABL Regulation by AXL Promotes Cisplatin Resistance in Esophageal Cancer Cancer Research 2013-01-01 [PMID: 23117882] (Immunohistochemistry, Human)
• Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis. J. Exp. Med., 2012-10-15;209(11):2033-47. 2012-10-15 [PMID: 23071254] (IHC-Fr, Neutralization, Human)
• Rachel M.A. Linger, Rebecca A. Cohen, Christopher T. Cummings, Susan Sather, Justine Migdall-Wilson, Deryck H.G. Middleton et al. Mer or Axl Receptor Tyrosine Kinase Inhibition Promotes Apoptosis, Blocks Growth, and Enhances Chemosensitivity of Human Non-Small Cell Lung Cancer Oncogene 2013-07-18 [PMID: 22890323]
• Zhang Z, Lee JC Activation of the AXL kinase causes resistance to EGFR-targeted therapy in lung cancer. Nat. Genet., 2012-07-01;44(8):852-860. 2012-07-01 [PMID: 22751098] (IHC-P, Western Blot, Human)
• Brown JE, Krodel M, Pazos M Cross-phosphorylation, signaling and proliferative functions of the Tyro3 and Axl receptors in Rat2 cells. PLoS ONE, 2012-05-14;7(5):e36800. 2012-05-14 [PMID: 22606290] (Immunoprecipitation, Human)
• Anjali Mishra, Jingcheng Wang, Yusuke Shiozawa, Samantha McGee, Jinkoo Kim, Younghun Jung et al. Hypoxia Stabilizes GAS6/Axl Signaling in Metastatic Prostate Cancer Molecular Cancer Research 2012-06-01 [PMID: 22516347]
• Migdall-Wilson J, Bates C, Schlegel J, Brandao L, Linger RM, Deryckere D, Graham DK Prolonged exposure to a Mer ligand in leukemia: Gas6 favors expression of a partial Mer glycoform and reveals a novel role for Mer in the nucleus. PLoS ONE, 2012-02-20;7(2):e31635. 2012-02-20 [PMID: 22363695] (Western Blot, Human)
• Niki A. Ottenhof, Folkert H. M. Morsink, Fiebo ten Kate, Cornelis J. F. van Noorden, G. Johan A. Offerhaus Multivariate analysis of immunohistochemical evaluation of protein expression in pancreatic ductal adenocarcinoma reveals prognostic significance for persistent Smad4 expression only Cell Oncol (Dordr) 2012-04-01 [PMID: 22351431]
• Shimojima M, Stroher U, Ebihara H, Feldmann H, Kawaoka Y Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry. J. Virol., 2011-12-07;86(4):2067-78. 2011-12-07 [PMID: 22156524] (Flow Cytometry, Human)
• Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques. Thromb. Haemost., 2011-11-10;107(1):140-9. 2011-11-10 [PMID: 22072012] (ICC, Human)
• Melinda A. Brindley, Catherine L. Hunt, Andrew S. Kondratowicz, Jill Bowman, Patrick L. Sinn, Paul B. McCray et al. Tyrosine kinase receptor Axl enhances entry of Zaire ebolavirus without direct interactions with the viral glycoprotein Virology 2011-07-05 [PMID: 21529875]
• Butler GS, Dean RA, Tam EM, Overall CM Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding. Mol. Cell. Biol., 2008-05-27;28(15):4896-914. 2008-05-27 [PMID: 18505826] (Western Blot, Human)
• Uehara H, Shacter E Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells. J. Immunol., 2008-02-15;180(4):2522-30. 2008-02-15 [PMID: 18250462] (Western Blot, Human)
• Collett GD, Sage AP, Kirton JP, Alexander MY, Gilmore AP, Canfield AE Axl/phosphatidylinositol 3-kinase signaling inhibits mineral deposition by vascular smooth muscle cells. Circ. Res., 2007-01-25;100(4):502-9. 2007-01-25 [PMID: 17255529] (Immunoprecipitation, Bovine)
• Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses. J. Thromb. Haemost., 2005-02-23;3(4):733-41. 2005-02-23 [PMID: 15733062] (Flow Cytometry, Neutralization, Human)

Reviews for Axl Antibody (AF154) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer IHC-Fr Human 01/05/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 23071254
• Species: Human

reviewed by: Verified Customer WB Human 01/05/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 20103767
• Species: Human

Bioinformatics

• Gene Symbol: AXL
• Uniprot:
  • AAA61243()