Axl Antibody [Biotin]

A431 (filled histogram) and Jurkat (open histogram) cells were stained with Goat Anti-Human Axl Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF154, filled histogram) followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Axl Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Axl
  Met1-Pro440
  Accession # AAA61243
• Specificity: Detects human Axl in ELISAs and Western blots. In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant mouse Axl, recombinant human (rh) Dtk, and rhMer is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AXL
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human Axl Antibody (Catalog # MAB154) ELISA Detection: Human Axl Biotinylated Antibody (Catalog # BAF154) Standard: Recombinant Human Axl Fc Chimera (Catalog # 154-AL)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Axl Antibody [Biotin]

• AI323647
• ARK
• AXL oncogene
• AXL receptor tyrosine kinase
• AXL transforming sequence/gene
• Axl
• EC 2.7.10
• EC 2.7.10.1
• JTK11
• Tyro7
• tyrosine-protein kinase receptor UFO
• UFO
• UFOoncogene AXL

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1,2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
3. Holland, S. et al. (2005) Canc. Res. 65:9294.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Axl Antibody (BAF154)(5)

Publications using BAF154

• Creixell, M;Taylor, SD;Gerritsen, J;Bae, SY;Jiang, M;Augustin, T;Loui, M;Boixo, C;Creixell, P;White, FM;Meyer, AS; Dissecting signaling regulators driving AXL-mediated bypass resistance and associated phenotypes by phosphosite perturbations bioRxiv : the preprint server for biology 2023-10-23 [PMID: 37961516] (ELISA Detection, Human)
• A Carestia, HA Mena, CM Olexen, JM Ortiz Wilc, S Negrotto, AE Errasti, RM Gómez, CN Jenne, EA Carrera Si, M Schattner Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice Cell Rep, 2019-07-23;28(4):896-908.e5. 2019-07-23 [PMID: 31340152] (Flow Cytometry, Human)
• Allison M. Claas, Lyla Atta, Simon Gordonov, Aaron S. Meyer, Douglas A. Lauffenburger Systems Modeling Identifies Divergent Receptor Tyrosine Kinase Reprogramming to MAPK Pathway Inhibition Cellular and Molecular Bioengineering 12/1/2018 [PMID: 30524510]
• Mihalis S. Kariolis, Yu Rebecca Miao, Douglas S. Jones, Shiven Kapur, Irimpan I. Mathews, Amato J. Giaccia et al. An engineered Axl 'decoy receptor' effectively silences the Gas6-Axl signaling axis Nature Chemical Biology 11/1/2014 [PMID: 25242553]
• Zizzo G, Guerrieri J, Dittman L, Merrill J, Cohen P Circulating levels of soluble MER in lupus reflect M2c activation of monocytes/macrophages, autoantibody specificities and disease activity. Arthritis Res Ther, 2013-01-01;15(6):R212. 2013-01-01 [PMID: 24325951] (Human)

Bioinformatics

• Gene Symbol: AXL
• Uniprot:
  • AAA61243()