Aurora A Antibody

Images

 
Western Blot: Aurora A Antibody [NB100-267] - Samples: Whole cell lysate (50 ug) from HeLa and HEPG2cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aurora A antibody used for WB at 0.2 ...read more
Immunocytochemistry/ Immunofluorescence: Aurora A Antibody [NB100-267] - Localization of Aurora A to Centrosomes asynchronous HeLa cells. NB 100-267 is diluted 1:1000. Aurora A(green) was visualized with FITC. DAPI ...read more
Western Blot: Aurora A Antibody [NB100-267] - Detection of Human and Mouse TSC1.
Western Blot: Aurora A Antibody [NB100-267] - Western blot and Immunoprecipitation detection of human 5TK15, using NB100-267. Samples: Whole cell lysate from 5KBr3 (A) or AsPC-1 (B) cells.
Western Blot: Aurora A Antibody [NB100-267] - Detection of Human Aurora A (STK15) on HeLa whole cell lysate using NB100-267. Immunoprecipitated Aurora A was detected using a monoclonal antibody to Aurora A from another ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Aurora A Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 75 and 125 of human Aurora A Kinase using the numbering given in entry NP_003591.2 (GeneID 6790).
Marker
Mitosis Marker
Specificity
This is specific for human STK15, Serine/Threonine Kinase 15, Aurora 2, BRAK, ARK1, Aurora-Related Kinase 1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AURKA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:250-1:1000
  • Immunoprecipitation 1-5 ug/mg lysate
Application Notes
By Western blot, this antibody recognizes bands around 48 kDa representing STK15.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Pancreas Lysate (NB820-59443)
MCF-7 Lysate (NB820-59465)
Aurora A Lysate (NBL1-07855)
Publications
Read Publications using NB100-267.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aurora A Antibody

  • AIK
  • AIKAurA
  • ARK1
  • ARK-1
  • ARK1EC 2.7.11.1
  • AURA
  • AURKA
  • Aurora A
  • aurora kinase AhARK1
  • aurora/IPL1-like kinase
  • Aurora/IPL1-related kinase 1
  • Aurora-related kinase 1
  • Breast tumor-amplified kinase
  • breast-tumor-amplified kinase
  • BTAK
  • BTAKAURORA2
  • EC 2.7.11
  • IPL1-related kinase
  • serine/threonine kinase 6
  • serine/threonine protein kinase 15
  • Serine/threonine-protein kinase 15
  • serine/threonine-protein kinase 6
  • Serine/threonine-protein kinase aurora-A
  • STK15
  • STK15serine/threonine kinase 15
  • STK6
  • STK6MGC34538
  • STK7

Background

Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aurora A Antibody (NB100-267)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AURKA
Entrez
Uniprot