Aurora A Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

Aurora A Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 75 and 125 of human Aurora A Kinase using the numbering given in entry NP_003591.2 (GeneID 6790).
• Marker: Mitosis Marker
• Specificity: This is specific for human STK15, Serine/Threonine Kinase 15, Aurora 2, BRAK, ARK1, Aurora-Related Kinase 1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AURKA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Aurora A Antibody [CoraFluor™ 1]

• AIK
• AIKAurA
• ARK1
• ARK-1
• ARK1EC 2.7.11.1
• AURA
• AURKA
• Aurora A
• aurora kinase AhARK1
• aurora/IPL1-like kinase
• Aurora/IPL1-related kinase 1
• Aurora-related kinase 1
• Breast tumor-amplified kinase
• breast-tumor-amplified kinase
• BTAK
• BTAKAURORA2
• EC 2.7.11
• IPL1-related kinase
• serine/threonine kinase 6
• serine/threonine protein kinase 15
• Serine/threonine-protein kinase 15
• serine/threonine-protein kinase 6
• Serine/threonine-protein kinase aurora-A
• STK15
• STK15serine/threonine kinase 15
• STK6
• STK6MGC34538
• STK7

Background

The protein encoded by the Aurora A gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AURKA