ATP6V1A Antibody - BSA Free

Western Blot: ATP6V1A Antibody [NBP1-33021] - Immunoblot for Voa and V1A subunits indicate presence of subunits during the CCV isolation procedure. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/32569#content) licensed under a CC-BY license.

Immunohistochemistry: ATP6V1A Antibody [NBP1-33021] - Mouse prostate. ATP6V1A antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: ATP6V1A Antibody [NBP1-33021] - Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE; antibody diluted at 1:5000.

Western Blot: ATP6V1A Antibody [NBP1-33021] - Various whole cell extracts (30ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody diluted by 1:10000.

Immunoprecipitation: ATP6V1A Antibody [NBP1-33021] - ATP6V1A Antibody [NBP1-33021] - Immunoprecipitation of ATP6V1A protein from HeLa whole cell extracts using 5 ug of ATP6V1A antibody. Western blot analysis was performed using ATP6V1A antibody EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Western Blot: ATP6V1A Antibody [NBP1-33021] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: ATP6V1A Antibody [NBP1-33021] - Functional analysis of the vATPase on CCVs.(A) Immunoblots of isolated SVs & CCVs for the clathrin light (LC) & heavy chains (HC), & SV marker proteins synaptophysin (Syph) & VAMP2 (left panel), as well as various Vo & V1 subunits of vATPase (right panel). (B) The ratio of the Vo & V1 as well as synaptophysin & VAMP2 (as SV markers) detected by immunoblotting in equal protein amount of CCVs & SVs (C) Normalized levels of Vo & V1 in CCV & SV samples, indicating that Vo:V1 ratio is 1:1 in both preparations. (D) ATPase activity measured in 1.3 µg of isolated SVs & CCVs. (E) Blocking percentage of ATPase activity by NEM (vATPase inhibitor) in 1.3 µg of SVs & CCVs. Error bars in (B–E) represent SD of 3–4 experimental replicates (p0.05 for C).Both Vo & V1 subunits are present on CCVs.Immunoblot for Voa & V1A subunits indicate presence of subunits during the CCV isolation procedure.ATPase activity measurements in isolated SVs.To make sure that the measured ATPase activity is within the standard curve range of the used kit, we tested different amounts of isolated SVs. The measurements of ATPase activity were then performed with 1.3 µg of SVs & CCVs which is in the liner range (inset). Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/32569), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, RM
• Applications: WB, ICC/IF, IHC, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ATP6V1A Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ATP6V1A. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP6V1A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reactivity reported in (PMID:21272169)
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockout Validated
  • Western Blot 1:1000-1:10000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for ATP6V1A Antibody - BSA Free

• 70kD, isoform 1
• ATP6A1
• ATP6V1A
• ATP6V1A1
• ATP6V1A1ATPase, H+ transporting, lysosomal, subunit A1
• ATPase, H+ transporting, lysosomal (vacuolar proton pump), alpha polypeptide
• ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
• EC 3.6.3
• EC 3.6.3.14
• EC 7.1.2.2
• H(+)-transporting two-sector ATPase, subunit A
• H+-transporting ATPase chain A, vacuolar (VA68 type)
• HO68
• VA68
• vacuolar ATP synthase catalytic subunit A, ubiquitous isoform
• Vacuolar ATPase isoform VA68
• vacuolar proton pump alpha subunit 1
• Vacuolar proton pump subunit alpha
• V-ATPase 69 kDa subunit 1
• V-ATPase 69 kDa subunit
• V-ATPase A subunit 1
• V-ATPase subunit A
• Vma1
• VPP2
• V-type proton ATPase catalytic subunit A

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATP6V1A Antibody (NBP1-33021)(2)

Publications using NBP1-33021

• Farsi Z, GowriSankaran S, Krunic M et al. Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity. Elife 2018-04-13 [PMID: 29652249] (Mouse)
• Bacac M, Fusco C, Planche A et al. Securin and separase modulate membrane traffic by affecting endosomal acidification Traffic 2011-05-01 [PMID: 21272169] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: ATP6V1A