ATP6V1B1 Antibody (OTI1H6)

Images

 
Western Blot: ATP6V1B1 Antibody (1H6) [NBP2-00580] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1B1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: ATP6V1B1 Antibody (1H6) [NBP2-00580] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP6V1B1.
Immunohistochemistry-Paraffin: ATP6V1B1 Antibody (1H6) [NBP2-00580] - Staining of paraffin-embedded Human Kidney tissue using anti-ATP6V1B1 mouse monoclonal antibody.
Flow Cytometry: ATP6V1B1 Antibody (1H6) [NBP2-00580] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATP6V1B1 antibody, and then ...read more
Immunohistochemistry-Paraffin: ATP6V1B1 Antibody (1H6) [NBP2-00580] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATP6V1B1 mouse monoclonal antibody.
Flow Cytometry: ATP6V1B1 Antibody (1H6) [NBP2-00580] - Analysis of Hela cells, using anti-ATP6V1B1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: ATP6V1B1 Antibody (1H6) [NBP2-00580] - Analysis of Jurkat cells, using anti-ATP6V1B1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI1H6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ATP6V1B1 Antibody (OTI1H6) Summary

Immunogen
Full length human recombinant protein of human ATP6V1B1 (NP_001683) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ATP6V1B1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
56.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-00580.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATP6V1B1 Antibody (OTI1H6)

  • ATP6B158kD subunit
  • ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
  • Endomembrane proton pump 58 kDa subunit
  • H+-ATPase beta 1 subunit
  • vacuolar proton pump 3
  • Vacuolar proton pump subunit B 1
  • vacuolar proton pump, subunit 3
  • VATBMGC32642
  • V-ATPase B1 subunit
  • V-ATPase subunit B 1
  • Vma2
  • V-type proton ATPase subunit B, kidney isoform

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATP6V1B1 Antibody (NBP2-00580)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATP6V1B1 Antibody (NBP2-00580) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70237AF350
Alexa Fluor 405 NBP2-70237AF405
Alexa Fluor 488 NBP2-70237AF488
Alexa Fluor 532 NBP2-70237AF532
Alexa Fluor 594 NBP2-70237AF594
Alexa Fluor 647 NBP2-70237AF647
Alexa Fluor 700 NBP2-70237AF700
Alexa Fluor 750 NBP2-70237AF750
Allophycocyanin NBP2-70237APC
Allophycocyanin/Cy7 NBP2-70237APCCY7
Biotin NBP2-70237B
DyLight 350 NBP2-70237UV
DyLight 405 NBP2-70237V
DyLight 488 NBP2-70237G
DyLight 550 NBP2-70237R
DyLight 594 NBP2-70237DL594
DyLight 650 NBP2-70237C
DyLight 680 NBP2-70237FR
DyLight 755 NBP2-70237IR
FITC NBP2-70237F
HRP NBP2-70237H
Janelia Fluor 549 NBP2-70237JF549
Janelia Fluor 646 NBP2-70237JF646
PE NBP2-70237PE
PE/Atto594 NBP2-70237PEATT594
PE/Cy5.5 NBP2-70237PECY55
PE/Cy7 NBP2-70237PECY7
PerCP NBP2-70237PCP

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Bioinformatics

Gene Symbol ATP6V1B1
Entrez