Genetic Strategies: Western Blot: ATP6V1A Antibody [NBP1-33021] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ...read more
Immunocytochemistry/ Immunofluorescence: ATP6V1A Antibody [NBP1-33021] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Western Blot: ATP6V1A Antibody [NBP1-33021] - Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE; antibody diluted at 1:5000.
Western Blot: ATP6V1A Antibody [NBP1-33021] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:10000.
Western Blot: ATP6V1A Antibody [NBP1-33021] - Various whole cell extracts (30ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody diluted by 1:10000.
Immunoprecipitation: ATP6V1A Antibody [NBP1-33021] - ATP6V1A Antibody [NBP1-33021] - Immunoprecipitation of ATP6V1A protein from HeLa whole cell extracts using 5 ug of ATP6V1A antibody. Western blot analysis was ...read more
68 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP1-33021 in the following applications:
ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
H(+)-transporting two-sector ATPase, subunit A
H+-transporting ATPase chain A, vacuolar (VA68 type)
vacuolar ATP synthase catalytic subunit A, ubiquitous isoform
Vacuolar ATPase isoform VA68
vacuolar proton pump alpha subunit 1
Vacuolar proton pump subunit alpha
V-ATPase 69 kDa subunit 1
V-ATPase 69 kDa subunit
V-ATPase A subunit 1
V-ATPase subunit A
V-type proton ATPase catalytic subunit A
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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