ATP6V1A Antibody

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Western Blot: ATP6V1A Antibody [NBP1-33021] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody. HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: ATP6V1A Antibody [NBP1-33021] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry: ATP6V1A Antibody [NBP1-33021] - Mouse prostate ATP6V1A antibody diluted at 1:500.
Western Blot: ATP6V1A Antibody [NBP1-33021] - Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE; antibody diluted at 1:5000.
Western Blot: ATP6V1A Antibody [NBP1-33021] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:10000.
Western Blot: ATP6V1A Antibody [NBP1-33021] - Various whole cell extracts (30ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody diluted by 1:10000.
Immunoprecipitation: ATP6V1A Antibody [NBP1-33021] - ATP6V1A Antibody [NBP1-33021] - Immunoprecipitation of ATP6V1A protein from HeLa whole cell extracts using 5 ug of ATP6V1A antibody. Western blot analysis was ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.57 mg/ml
     

Genetic Strategies

   

Order Details

ATP6V1A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ATP6V1A. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP6V1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-33021 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%), Xenopus laevis (90%), Chicken (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.57 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATP6V1A Antibody

  • 70kD, isoform 1
  • ATP6A1
  • ATP6V1A1ATPase, H+ transporting, lysosomal, subunit A1
  • ATPase, H+ transporting, lysosomal (vacuolar proton pump), alpha polypeptide
  • ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
  • EC 3.6.3
  • EC 3.6.3.14
  • H(+)-transporting two-sector ATPase, subunit A
  • H+-transporting ATPase chain A, vacuolar (VA68 type)
  • HO68
  • VA68
  • vacuolar ATP synthase catalytic subunit A, ubiquitous isoform
  • Vacuolar ATPase isoform VA68
  • vacuolar proton pump alpha subunit 1
  • Vacuolar proton pump subunit alpha
  • V-ATPase 69 kDa subunit 1
  • V-ATPase 69 kDa subunit
  • V-ATPase A subunit 1
  • V-ATPase subunit A
  • Vma1
  • VPP2
  • V-type proton ATPase catalytic subunit A

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATP6V1A Antibody (NBP1-33021)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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Bioinformatics

Gene Symbol ATP6V1A