ATP5A Antibody - BSA Free

Western Blot: ATP5A Antibody [NBP2-38525] - Analysis using Anti-ATP5A1 antibody NBP2-38525 (A) shows similar pattern to independent antibody NBP2-38470 (B).

Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human kidney.

Western Blot: ATP5A Antibody [NBP2-38525] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Immunohistochemistry: ATP5A Antibody [NBP2-38525] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human colon, heart muscle, kidney and liver using Anti-ATP5A1 antibody NBP2-38525 (A) shows similar protein distribution across tissues to independent antibody NBP2-38470 (B).

Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human liver.

Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human heart muscle.

Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-38525] - Staining of human colon.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ATP5A Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: SILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVD
• Predicted Species: Mouse (99%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP5F1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  ATP5A Protein (NBP2-38525PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ATP5A Antibody - BSA Free

• ATP synthase alpha chain, mitochondrial
• ATP synthase subunit alpha, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F1 co
• ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1
• ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform1, cardiac muscle
• ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform2, non-cardiac muscle-like 2
• ATP sythase (F1-ATPase) alpha subunit
• ATP5A
• ATP5A1
• ATP5AL2
• ATP5AMOM2
• ATPM
• ATPMATP synthase subunit alpha, mitochondrial
• cardiac muscle
• COXPD22
• EC 3.6.3
• EC 3.6.3.14
• epididymis secretory sperm binding protein Li 123m
• hATP1
• HEL-S-123m
• MC5DN4
• MC5DN4A
• MC5DN4B
• mitochondrial ATP synthetase, oligomycin-resistant
• MOM2
• OMR
• ORM
• ORMATP synthase alpha chain, mitochondrial

Background

ATP5A encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the same protein have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP5F1A
• Uniprot:
  • P25705(Human)