ATG4A Antibody - BSA Free

Western Blot: ATG4A Antibody [NBP2-81788] - Analysis of ATG4A in EL4 cell lysate with ATG4A antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry: ATG4A Antibody - BSA Free [NBP2-81788] - Immunohistochemistry of ATG4A in human spleen tissue with ATG4A antibody at 5 ug/ml.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ATG4A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ATG4A Antibody - BSA Free (NBP2-81788) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: ATG4A antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ATG4A. The immunogen is located within the last 50 amino acids of ATG4A. Amino Acid Squence: VQKHPSHWPPFVPPAKPE
• Specificity: ATG4A antibody is human, mouse and rat reactive. At least two isoforms of ATG4A are known to exist; this antibody will detect both isoforms. ATG4A is predicted to not cross-react with other ATG4 proteins.
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATG4A
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ATG4A Antibody - BSA Free

• APG4 autophagy 4 homolog A (S. cerevisiae)
• Apg4a
• ATG4 autophagy related 4 homolog A (S. cerevisiae)
• ATG4A
• Atg4al
• Autl2
• AUT-like 2 cysteine endopeptidase
• AUT-like 2, cysteine endopeptidase (S. cerevisiae)
• Autophagin 2
• autophagin-2
• cysteine endopeptidase
• cysteine protease ATG4A
• EC 3.4.22
• EC 3.4.22.-
• hAPG4A

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified.; FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.; ENZYME REGULATION: Inhibited by N-ethylmaleimide; SUBCELLULAR LOCATION: Cytoplasm;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATG4A