ATF4 [p Ser245] Antibody

Images

 
Western Blot: ATF4 [p Ser245] Antibody [NB100-81802] - Analysis of extracts from Hela cells untreated or treated with UV using NB100-81802
Immunohistochemistry: ATF4 [p Ser245] Antibody [NB100-81802] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ATF4 (phospho-Ser245) antibody NB100-81802.
Western Blot: ATF4 [p Ser245] Antibody [NB100-81802] - Western Blot analysis of various cell lysis using NB100-81802.
Immunohistochemistry-Paraffin: ATF4 [p Ser245] Antibody [NB100-81802] - Immunohistochemical staining in human breast cancer formalin fixed paraffin-embedded tissue section.
Immunocytochemistry/ Immunofluorescence: ATF4 [p Ser245] Antibody [NB100-81802] - Immunofluorescent staining in A549 cells.
Western Blot: ATF4 [p Ser245] Antibody [NB100-81802] - Western Blot analysis of various cell lysis using NB100-81802.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

ATF4 [p Ser245] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human ATF4 around the phosphorylation site of serine 245 (N-R-SP-L-P).
Modification
p Ser245
Specificity
Detects endogenous levels of ATF4 only when phosphorylated at serine 245.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATF4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB100-81802.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATF4 [p Ser245] Antibody

  • activating transcription factor 4 (tax-responsive enhancer element B67)
  • Activating transcription factor 4
  • ATF4
  • cAMP-dependent transcription factor ATF-4
  • cAMP-responsive element-binding protein 2
  • CREB-2DNA-binding protein TAXREB67
  • cyclic AMP-dependent transcription factor ATF-4
  • Cyclic AMP-responsive element-binding protein 2
  • TAXREB67
  • TAXREB67CREB2cAMP response element-binding protein 2
  • Tax-responsive enhancer element-binding protein 67
  • TXREB

Background

ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain (referenced from Entrez gene).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATF4 Antibody (NB100-81802)(1)

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATF4 Antibody (NB100-81802) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for ATF4 Antibody (NB100-81802)

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Blogs on ATF4.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

CENPF Antibodies as Potential Cancer Markers
Centromere protein F (CENPF), also named mitosin, is a large human protein of 3113 amino acid residues. Its expression and localization are cell cycle-dependent. The protein levels are low in G1 phase but elevated from S to early M phase. CENPF is a n...  Read full blog post.

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Bioinformatics

Gene Symbol ATF4
Entrez
Uniprot