ATF4 [p Ser245] Antibody

Western Blot: ATF4 [p Ser245] Antibody [NB100-81802] - Analysis of extracts from Hela cells untreated or treated with UV using ATF4 (Phospho-Ser245) Antibody

Immunohistochemistry: ATF4 [p Ser245] Antibody [NB100-81802] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ATF4 (phospho-Ser245) antibody NB100-81802.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

ATF4 [p Ser245] Antibody Summary

• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human ATF4 around the phosphorylation site of serine 245 (N-R-SP-L-P).
• Modification: p Ser245
• Specificity: Detects endogenous levels of ATF4 only when phosphorylated at serine 245.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATF4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ATF4 [p Ser245] Antibody

• activating transcription factor 4 (tax-responsive enhancer element B67)
• Activating transcription factor 4
• ATF4
• cAMP-dependent transcription factor ATF-4
• cAMP-responsive element-binding protein 2
• CREB-2DNA-binding protein TAXREB67
• cyclic AMP-dependent transcription factor ATF-4
• Cyclic AMP-responsive element-binding protein 2
• TAXREB67
• TAXREB67CREB2cAMP response element-binding protein 2
• Tax-responsive enhancer element-binding protein 67
• TXREB

Background

ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain (referenced from Entrez gene).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATF4
• Entrez:
  • 468(Human)
• Uniprot:
  • P18848(Human)