TRIB3 Antibody

Western Blot: TRIB3 Antibody [NB100-56398] - Analysis of TRIB3 in human liver lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRIB3 Antibody Summary

• Description: Novus Biologicals Rabbit TRIB3 Antibody (NB100-56398) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-TRIB3 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to amino acid residues 315-332 of human TRIB3. Genbank accession number: NM_021158.
• Specificity: This detects a protein of approximate mol. wt. of 40 kDa in human liver lysate.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIB3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1-3 ug/ml
• Application Notes: )
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)
  TRIB3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for TRIB3 Antibody

• C20orf97
• dJ1103G7.3
• neuronal cell death inducible putative kinase
• Neuronal cell death-inducible putative kinase
• NIPK
• p65-interacting inhibitor of NF-kappaB
• p65-interacting inhibitor of NF-kappa-B
• SINK
• SKIP3
• TRB-3
• TRB3chromosome 20 open reading frame 97
• tribbles homolog 3 (Drosophila)
• tribbles homolog 3

Background

A novel inhibitor of cell division, Tribbles, blocks mitosis at a critical point in Drosophila morphogenesis. Recently, mammalian homologues of Tribbles (Trbs) have been identified that control mitogen-activated protein kinase (MAPK) activity. TRIB3 also known as TRB-3, SINK, and SKIP3 is a member of Trbs family. Overexpression of this protein inhibits NF-kB-dependent transcription induced by tumor necrosis factor (TNF) stimulation or its downstream signaling proteins but does not inhibit NF-kB translocation to the nucleus and binding to DNA. TRIB3/SKIP3 is also overexpressed in multiple human tumors and is regulated by hypoxia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIB3 Antibody (NB100-56398)(4)

Publications using NB100-56398

• Lin Rong-Jaan, Wu I-Jung, Hong Jo-Yu et al. Capsaicin-induced TRIB3 upregulation promotes apoptosis in cancer cells. Scientific Reports 2018-10-04 [PMID: 30323679] (WB, Human)
• Guo L, Guo ZX, Gong HP et al. Tribbles homolog 3 is induced by high glucose and associated with apoptosis in human endothelial cells Mol Med Rep 2015-03-31 [PMID: 25845379] (WB, ICC/IF, Human)
• Wang ZH, Shang YY, Zhang S et al. Silence of TRIB3 suppresses atherosclerosis and stabilizes plaques in diabetic ApoE-/-/LDL receptor-/- mice. Diabetes. 2012-02-01 [PMID: 22275087] (WB)
  
  Details:
  TRIB3(IMG-5118A). WB: Mouse (On a high fat diet) peritoneal macrophage tissues, Fig5.
• Loewen N, Chen J, Dudley VJ et al. Genomic response of hypoxic Muller cells involves the very low density lipoprotein receptor as part of an angiogenic network. Exp Eye Res. 2009-05-01 [PMID: 19233325] (ICC/IF)
  
  Details:
  TRIB3 (IMG-5118A). IF/ICC: Muller (rMC-1) cell line exposed to hypoxic conditions, Fig 6.

Bioinformatics

• Gene Symbol: TRIB3
• Entrez:
  • 57761(Human)
• Uniprot:
  • Q96RU7(Human)