GADD34 Antibody

Images

 
Western Blot: GADD34 Antibody [NB100-778] - (0.1ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: GADD34 Antibody [NB100-778] - (2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GADD34 Antibody Summary

Immunogen
Peptide with sequence C-AAALDLSGRRG corresponding to C-Terminus according to NP_055145.3.
Marker
ER Stress Marker
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PPP1R15A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:128000
Application Notes
ICC/IF: Not suitable due to some low non-specific signals seen in WB. WB: Approx. 75 kDa band observed in HepG2 lysates (calculated MW of 73.4 kDa band according to NP_055145.2). IHC-P: Human liver shows cytoplasmic staining consistent with ER in the hepatocytes.
Positive Control
GADD34 Lysate (NBL1-14676)
Reviewed Applications
Read 1 Review rated 4
using
NB100-778 in the following applications:

Publications
Read Publications using NB100-778.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GADD34 Antibody

  • GADD34Growth arrest and DNA damage-inducible protein GADD34
  • growth arrest and DNA-damage-inducible 34
  • Myeloid differentiation primary response protein MyD116 homolog
  • protein phosphatase 1 regulatory subunit 15A
  • protein phosphatase 1, regulatory (inhibitor) subunit 15A

Background

GADD34 is a phosphatase whose expression is increased by exposure of cells to DNA damage or stress induced growth arrest. Induction is correlated with apoptosis following exposure to ionizing radiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, IHC-P, PEP-ELISA

Publications for GADD34 Antibody (NB100-778)(2)

Review for GADD34 Antibody (NB100-778) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: WB.

Reviews using NB100-778:
Filter by Applications
WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Other 12/09/2009
View

Summary

ApplicationWestern Blot
Sample Testedwhole cell lysate, Sample Amount: 40 ug
SpeciesOther

Blocking

Blocking DetailsBlocking Buffer: 5mg/ml BSA in TBST, Blocking Time: 2 hours, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1/1000, Incubation Dilution Buffer: 1% BSA in TBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: Donkey polyclonal anti-goat IgG (H+L) conj to HRP, Secondary Ab Dilution Ratio: 1/2000
Secondary Manufacturer Cat#NB7379

Details

Detection NotesDetection Method: ECL, Exposure Time: 1 minute, Specific Bands: 75 kDa, Non-Specific Bands: 45 55 kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GADD34 Antibody (NB100-778). (Showing 1 - 1 of 1 FAQs).

  1. We are interested in your items, NB100-778. Would you please let us know the % of predicted cross reactivity to drosophila based on sequence homology? http://www.uniprot.org/uniprot/Q9W1E4
    • Unfortunately, the immunogen of NB100-778 is only about 36% homologous to the corresponding region of the Drosophila sequence. Based on this information, I would not recommend this product for use in Drosophila.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Anonymous
12/09/2009
Application: WB
Species: Other

Bioinformatics

Gene Symbol PPP1R15A
Entrez
Uniprot