ASC/TMS1 Antibody

Images

 
Western Blot: ASC/TMS1 Antibody [NBP2-48925] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: ASC/TMS1 Antibody [NBP2-48925] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleus, nucleoli & cytosol.
Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Staining in human spleen and skeletal muscle tissues using anti-PYCARD antibody. Corresponding PYCARD RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: ASC/TMS1 Antibody [NBP2-48925] - Staining of human spleen shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ASC/TMS1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS
Specificity
Specificity of human ASC/TMS1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PYCARD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ASC/TMS1 Recombinant Protein Antigen (NBP2-48925PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ASC/TMS1 Antibody

  • ASC
  • ASCMGC10332
  • CARD5
  • CARD5TMS
  • caspase recruitment domain protein 5
  • Caspase recruitment domain-containing protein 5
  • hASC
  • PYCARD
  • PYD and CARD domain containing
  • PYD and CARD domain-containing protein
  • Target of methylation-induced silencing 1
  • TMS1
  • TMS-1
  • TMS1apoptosis-associated speck-like protein containing a CARD

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for ASC/TMS1 Antibody (NBP2-48925)

Discover related pathways, diseases and genes to ASC/TMS1 Antibody (NBP2-48925). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for ASC/TMS1 Antibody (NBP2-48925)

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PTMs for ASC/TMS1 Antibody (NBP2-48925)

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Research Areas for ASC/TMS1 Antibody (NBP2-48925)

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Blogs on ASC/TMS1.

Immunity’s flipside: Microglia promote Alzheimer’s pathology during inflammation
By Jamshed Arslan Pharm.D. Microglia are brain's macrophages. In Alzheimer's disease (AD), microglia clear up protein aggregates called amyloid beta plaques. The connection between immune activation and AD is unclea...  Read full blog post.

CARD & NFKB Antibodies for Apoptosis Research
Apoptosis is one of the main types of programmed cell death which involves a cascade of biochemical events leading to specific cell morphology characteristics and ultimately death of cells.Caspases play crucial roles in modulating cellular signaling...  Read full blog post.

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Bioinformatics

Gene Symbol PYCARD
Entrez