ASC/TMS1 Antibody

Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP1-78978] - Tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78978] - Analysis of ASC/TMS1 in mouse intestine using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu
• Applications: ICC/IF, IHC, IHC-P, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.12 mg/ml

ASC/TMS1 Antibody Summary

• Immunogen: A synthetic peptide made to a C-terminal portion of the human ASC/TMS1 protein (between residues 145-195) [Uniprot: Q9ULZ3]
• Localization: Cytoplasm. Note: Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PYCARD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:40-1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
• Application Notes: Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not recommended for Western Blot.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.12 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ASC/TMS1 Antibody

• ASC
• ASCMGC10332
• CARD5
• CARD5TMS
• caspase recruitment domain protein 5
• Caspase recruitment domain-containing protein 5
• hASC
• PYCARD
• PYD and CARD domain containing
• PYD and CARD domain-containing protein
• Target of methylation-induced silencing 1
• TMS1
• TMS-1
• TMS1apoptosis-associated speck-like protein containing a CARD

Background

Target of methylation-induced silencing 1 protein, TMS1 (ASC, Pycard) is a 21 kDa cytoplasmic adaptor protein containing 2 protein-protein interaction domains: an N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). Both domains are members of the six-helix bundle death domain superfamily. These domains, via caspase activation, mediate the assembly of large signaling complexes that are involved in the apoptotic and inflammatory signaling pathways. During apoptosis TMS1 aggregates near the nuclear periphery as ball-like structures. Recent research suggests that it can modulate MAPK expression by suppressing the DUSP10/MKP5 phosphatase thus allowing the phosphorylation of MAPK by pathogens and toll-like receptor agonists. PMID: 2148701.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASC/TMS1 Antibody (NBP1-78978)(1)

Publication using NBP1-78978

• Yang Q, Liu Q, Lv H, et al. Effect of pulegone on the NLPR3 inflammasome during inflammatory activation of THP 1 cells Exp Ther Med Dec 13 2019 [PMID: 32010303]

Review for ASC/TMS1 Antibody (NBP1-78978) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous WB Mouse 10/06/2015

Summary

• Application: Western Blot
• Sample Tested: mouse bone marrow-derived macrophage cells
• Species: Mouse

Blocking

• Blocking Details: 5% skim milk in PBS-T buffer

Primary Anitbody

• Dilution Ratio: 1 ug/ml

Secondary Antibody

• Secondary Description: Goat anti-mouse HRP, 1:4,000
• Secondary Concentration: 0.4 ug/ml

Details

• Detection Notes: western blot

Bioinformatics

• Gene Symbol: PYCARD
• Entrez:
  • 29108(Human)
• Uniprot:
  • Q9ULZ3()