ASC/TMS1 Antibody

Images

 
Western Blot: ASC/TMS1 Antibody [NBP1-77297] - Western blot analysis of ASC in HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide with ASC antibody at 1 ug /ml.
Immunocytochemistry/ Immunofluorescence: ASC/TMS1 Antibody [NBP1-77297] - HL60 cells with ASC/TMS1 antibody at 5 ug/ml.
Immunohistochemistry: ASC/TMS1 Antibody [NBP1-77297] - Staining of human spleen tissue with antibody at 2.5 ug/ml.
Immunofluorescence: ASC/TMS1 Antibody [NBP1-77297] - ASC in human spleen tissue with ASC antibody at 20 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ASC/TMS1 Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid peptide near the carboxy terminus of human ASC. The immunogen is located within the last 50 amino acids of ASC.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PYCARD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
ASC antibody can be used for detection of ASC/TMS1 by Western blot at 1 mg/ml. An approximately 25 kDa band can be detected.
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ASC/TMS1 Peptide (NBP1-77297PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASC/TMS1 Antibody

  • ASC
  • ASCMGC10332
  • CARD5
  • CARD5TMS
  • caspase recruitment domain protein 5
  • Caspase recruitment domain-containing protein 5
  • hASC
  • PYCARD
  • PYD and CARD domain containing
  • PYD and CARD domain-containing protein
  • Target of methylation-induced silencing 1
  • TMS1
  • TMS-1
  • TMS1apoptosis-associated speck-like protein containing a CARD

Background

Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1. Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3,4). Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on ASC/TMS1.

CARD & NFKB Antibodies for Apoptosis Research
Apoptosis is one of the main types of programmed cell death which involves a cascade of biochemical events leading to specific cell morphology characteristics and ultimately death of cells.Caspases play crucial roles in modulating cellular signaling...  Read full blog post.

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Bioinformatics

Gene Symbol PYCARD
Entrez
Uniprot