ASAP1 Antibody

Western Blot: ASAP1 Antibody [NBP1-19159] - ASAP1 by Western Blot and Immunoprecipitation: [NBP1-19159]- Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ASAP1 was also immunoprecipitated by rabbit anti-ASAP1 antibody NBP1-19158, which recognizes an upstream epitope.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ASAP1 Antibody Summary

• Description: Novus Biologicals Rabbit ASAP1 Antibody (NBP1-19159) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 750 and 800 of human ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 using the numbering given in entry NP_060952.2 (GeneID 50807).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ASAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ASAP1 Antibody

• 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activatingprotein
• AMAP1
• ARF GTPase-activating protein 1
• arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
• ArfGAP with SH3 domain, ankyrin repeat and PH domain 1
• centaurin, beta 4
• CENTB4
• DDEF1ADP-ribosylation factor-directed GTPase-activating protein 1
• development and differentiation enhancing factor 1
• Development and differentiation-enhancing factor 1
• Differentiation-enhancing factor 1
• KIAA1249DEF-1
• PAG2
• PAP
• PIP2-dependent ARF1 GAP
• ZG14P

Background

ASAP1 ( Arf-Gap with SH3 domain, ANK repeat and PH domain-containing protein 1) is an Arf (ADP-ribosylation factor) GAP (GTPase-activating protein) that catalyzes the hydrolysis of GTP bound to Arf, a small GTP-binding protein. As a regulator of Arf activity ASAP1 has been shown to regulate cell adhesion and migration, and to be involved in carcinogenesis. Structurally, ASAP1 bears two ankyrin repeats, one Arf-GAP domain, one PH domain, one SH3 domain, and an N-terminal Bin, Amphiphysin and Rvs167 (BAR) domain. Alternative names for ASAP1 include PIP2-dependent ARF1 GAP, ARF GTPase-activating protein 1, development and differentiation-enhancing factor 1, differentiation-enhancing factor 1, PAP, PAG2, AMAP1, ZG14P, CENTB4, DEF-1, DDEF1, and KIAA1249.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASAP1
• Entrez:
  • 50807(Human)
• Uniprot:
  • NP_060952.2(Human)