ARNT/HIF-1 beta Antibody (2B10)

Images

 
Western Blot: ARNT/HIF-1 beta Antibody (2B10) [NB300-525] - Analysis of COS cell extract.
Immunocytochemistry/ Immunofluorescence: ARNT/HIF-1 beta Antibody (2B10) [NB300-525] - Analysis of HIF-1 beta (green) in NRK cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% ...read more
Immunocytochemistry/ Immunofluorescence: ARNT/HIF-1 beta Antibody (2B10) [NB300-525] - HIF-1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides ...read more
Immunocytochemistry/ Immunofluorescence: ARNT/HIF-1 beta Antibody (2B10) [NB300-525] - HIF-1 beta Antibody (2B10) [NB300-525] - HIF-1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is ...read more
Immunocytochemistry/ Immunofluorescence: ARNT/HIF-1 beta Antibody (2B10) [NB300-525] - HIF-1 beta Antibody (2B10) [NB300-525] - HIF-1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Am, Pm, ZeSpecies Glossary
Applications WB, GS, ICC/IF, IHC, IHC-P, IP
Clone
2B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ARNT/HIF-1 beta Antibody (2B10) Summary

Immunogen
Synthetic peptide corresponding to residues C N(771) S Y N N E E F P D L T M F P P F S E(789) of human ARNT.
Localization
Nuclear
Specificity
Detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ARNT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:3000
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10 - 1:500
Application Notes
May be useful in ELISA, IF, IP, Gel Super Shift Assays, and WB. ChIP usage was reported in scientific literature. WB: Detects an approx. 87 kDa protein representing ARNT in COS cell extract. IF: Staining of ARNT in VT{2} cells (Hepa 1-C4T mutant deficient in ARNT function but stably transfected with the full length human ARNT cDNA) using NB 300-525 yields non-nucleolar, nuclear staining only.
Positive Control
ARNT/HIF-1 beta Lysate (NBL1-07720)
Reviewed Applications
Read 1 Review rated 4
using
NB300-525 in the following applications:

Publications
Read Publications using
NB300-525 in the following applications:

  • 1 publication
  • WB
    3 publications

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 8000031). Clawed frog reactivity reported in scientific literature (PMID: 14984034). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1.0 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARNT/HIF-1 beta Antibody (2B10)

  • ARNT protein
  • ARNT
  • aryl hydrocarbon receptor nuclear translocator
  • BHLHE2
  • HIF1 beta
  • HIF-1 beta
  • HIF1B
  • HIF1BETA
  • HIF-1-beta
  • HIF1-beta
  • hypoxia-inducible factor 1, beta subunit
  • Hypoxia-inducible factor 1-beta
  • nuclear translocator
  • TANGO

Background

sHIF1 beta (hypoxia-inducible factor 1 beta, or aryl hydrocarbon receptor nuclear transporter, ARNT) is a transcription factor that contains a basic Helix-Loop-Helix (bHLH) motif as well as two PAS domains. HIF1 beta is required for the activity of the aryl hydrocarbon (Ah) receptor, which is involved in the induction of several enzymes that are involved in xenobiotic metabolism. Binding of ligands such as dioxin to the Ah receptor result in the translocation of the Ah receptor from the cytosol to the nucleus through its association with HIF1 beta. This complex then initiates transcription of genes involved in the activation of PAH procarcinogens. Efficient DNA binding of HIF1 beta requires heterodimerisation with another bHLH protein, such as the Ah receptor. HIF1 beta also plays a critical role in the transcriptional regulation of the adaptive response to hypoxia by forming heterodimers with HIF1 alpha and EPAS1/HIF2 alpha. Hypoxia-induced gene transcription contributes substantially to the malignant phenotype of solid tumours by promoting characteristic tumour behaviour such as angiogenesis, invasion, metastasis, de-differentiation and enhanced glycolytic metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Am, Pm, Ze
Applications: WB, GS, ICC/IF, IHC, IHC-P, IP

Publications for ARNT/HIF-1 beta Antibody (NB300-525)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ChIP, WB.


Filter By Application
ChIP
(1)
WB
(3)
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Human
(4)
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Showing Publications 1 - 7 of 7.
Publications using NB300-525 Applications Species
Mandl M, Depping R. ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells. Cancer Cell Int. 2017 Aug 31 [PMID: 28855849] (WB, Human) WB Human
Mandl M, Lieberum MK, Depping R. A HIF-1alpha-driven feed-forward loop augments HIF signalling in Hep3B cells by upregulation of ARNT. Cell Death Dis 2016 Jun 30 [PMID: 27362802]
Mandl M, Lieberum M, Dunst J, Depping R. The expression level of the transcription factor Aryl hydrocarbon receptor nuclear translocator (ARNT) determines cellular survival after radiation treatment. Radiat Oncol. 2015 Nov 17 [PMID: 26572229] (WB, Human) WB Human
Thomas S, Harding MA, Smith SC et al. CD24 Is an Effector of HIF-1-Driven Primary Tumor Growth and Metastasis Cancer Res 2012 Oct 22 [PMID: 22926560] (ChIP, Human) ChIP Human
Singh KP, Wyman A, Casado FL et al. Treatment of mice with the Ah receptor agonist human carcinogen dioxin results in altered numbers function of hematopoietic stem cells. Carcinogenesis;30(1):11-19. 2009 [PMID: 18820284]
Amir S, Wang R, Simons JW et al. Sept9_v1 upregulates HIF-1 by preventing its rack1-mediated degradation. J Biol Chem;284(17):11142-51. 2009 Apr 24. [PMID: 7526149]
Wolff MH. et al. In vitro characterization of the interaction of hypoxia - inducible factors and estrogen receptors in breast cancer / In vitro Charakterisierung des Zusammenspiels der Hypoxie-Induzierbaren-Faktoren und der Ostrogenrezeptoren beim Mammakarzinom. PhD Thesis, Aus der Sektion Medizin, University of Lubeck, Germany [PI: Univ. Prof. Dr. med. Wolfgang Jelkmann] Link: http://www.students.informatik.uni-luebeck.de/zhb/ediss1485.pdf Thesis. 2015 (WB, Human) WB Human

Review for ARNT/HIF-1 beta Antibody (NB300-525) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: ELISA.

Reviews using NB300-525:
Filter by Applications
ELISA
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ELISA Other 06/04/2009
View

Summary

ApplicationELISA
Sample TestedGuinea Pig Liver Extract
SpeciesOther
Lot187-112
CommentsThis was used for Direct ELISA assay.

Primary Anitbody

Dilution RatioPrimary Ab Incubation Dilution Buffer: Tris/NaCl, Primary Ab Dilution Ratio: 1:1000, Incubation Time: 1 hr, Incubation Temp: 70F

Comments

CommentsThis was used for Direct ELISA assay.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ARNT/HIF-1 beta Antibody (NB300-525) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on ARNT/HIF-1 beta.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.

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Recent Reviews

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4
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2
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1
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Anonymous
06/04/2009
Application: ELISA
Species: Other

Bioinformatics

Gene Symbol ARNT
Entrez
Uniprot